Human profibrinolysin kringle 5 deletion mutation recombinant polypeptide

A human plasminogen, recombinant polypeptide technology, applied in the direction of recombinant DNA technology, enzymes, enzymes, etc., can solve the problems of vascular leakage without satisfactory treatment methods, etc.

Inactive Publication Date: 2003-10-29
QIYUAN BIOLOGICAL SCI & TECH GUANGZHOU CITY
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  • Human profibrinolysin kringle 5 deletion mutation recombinant polypeptide
  • Human profibrinolysin kringle 5 deletion mutation recombinant polypeptide
  • Human profibrinolysin kringle 5 deletion mutation recombinant polypeptide

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Embodiment Construction

[0016] Materials: Retinal capillary endothelial cells (RCEC) and epithelial cells were isolated from bovine eyes as described previously (Grant, M.B. et al. Invest Ophthalmol. Vis. Sci., 32:53-64, 1991). It is shaped like a pebble, bound to fluorescent probe DiI-labeled acetylated low-density lipoprotein (Biomedical Technologies Inc., Stoughton, MA), confirmed as RCEC, cultured epithelial cell purity by α-smooth muscle actin-specific FITC binding Antibody identification. Brown-Norway rats were purchased from Harlan (Indianapolis, IN).

[0017] 1. Construction of K5mut1:

[0018]Human K5 cDNA was amplified from liver RNA by RT-PCR (Ma J.X. et al. FEBS Lett, 452:199-204, 1999). The deletion mutant was amplified by PCR using the human K5 template. The 5'PCR primer (5'-ATGAATTCGTGTATGTTTGGGAATGGG-3') and 3'PCR primer (5'-GCCAAGCTTACACTGAGGGACATCACAGTAG-3') each contained EcoR I and HindIII sites to facilitate cloning. PCR deleted the nucleotide sequence encoding 10 amino acid ...

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Abstract

A human plasminogen Kringle 5 deleted mutant recombinant polypeptide for treating the retinopath, such as vascular hyperplasia and angiorrhea, is prepared through removing 15 amino acid residue from K5 molecule while retaining 3 disulfide bond, expressing in colibacillus, and purifying by affinity chromatography.

Description

technical field [0001] The present invention relates to a mutant of human plasminogen Kringle5 with antiangiogenic activity, in particular to a human plasminogen Kringle5 deletion mutant recombinant polypeptide (K5mut1 for short). Background technique [0002] Angiogenesis is the process of sprouting new blood vessels from the existing capillary network. Angiogenesis has the positive effect of restoring blood supply and promoting wound healing in trauma and peripheral circulatory occlusion. In this case, it is a strictly controlled process; but in more cases, angiogenesis is a continuous process. , uncontrolled process, has become one of the main pathological features of some major diseases such as tumors, rheumatoid arthritis and diabetic retina. Angiogenesis is a common symptom and cause of malignant tumors and other angioproliferative diseases. Targeting angiogenesis is becoming a research hotspot in the treatment of malignant tumors and other angioproliferative diseases...

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Application Information

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IPC IPC(8): C12N1/20C12N9/68C12N15/12C12N15/70
Inventor 高国全马建兴刘祖国李民友
Owner QIYUAN BIOLOGICAL SCI & TECH GUANGZHOU CITY
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