Yak-blood deproteined extract and its extraction method and use thereof

A blood extract and protein-removing technology, which can be used in drug combinations, cardiovascular system diseases, and medical raw materials derived from mammals, can solve problems such as poor efficacy

Inactive Publication Date: 2004-01-28
魏战增
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, some scholars have noticed that the deproteinized extract of adult bovine blood also has the above bio...

Method used

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Experimental program
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Effect test

Embodiment 1

[0024] The yak blood deproteinized extract of the present invention can be obtained by the following preparation method:

[0025] (1) Collect yak blood under sterile conditions, centrifuge at 4000rpm for 20 minutes at a temperature of 4°C, and obtain a supernatant.

[0026] (2), take the supernatant liquid, use 0.3 micron membrane filtration to clarify, get clear liquid.

[0027] (3) Milipore ultrafilter is used in the clarified solution to cut off components with a molecular weight less than 6000 Daltons (the ultrafiltration rate is 200ml / min, and the peristaltic pump pressure is ≤0.2mpa).

[0028] (4), subpackage, freeze-drying.

Embodiment 2

[0030] The yak blood deproteinized extract of the present invention can also be obtained by the following preparation method:

[0031] (1) Collect yak blood under sterile conditions, centrifuge at 4000 rpm for 20 minutes at a temperature of 4° C. to obtain a supernatant.

[0032] (2) Take the supernatant, add 95% ethanol twice the serum volume, keep stirring for 60 minutes to denature and precipitate the protein, and then centrifuge at 20,000 rpm for 30 minutes at 4°C to leave the supernatant.

[0033] (3), using a vacuum rotary evaporator, 40 ° C, 1 atmosphere of pressure to remove ethanol.

[0034] (4) The supernatant uses a Milipore ultrafilter to cut off components with a molecular weight less than 6000 Daltons (the ultrafiltration rate is 200ml / min, and the peristaltic pump pressure is ≤0.2mpa).

[0035] (5), subpackage, freeze-drying.

Embodiment 3

[0037] The effect of the deproteinized yak blood extract of the present invention on the acute hypoxia tolerance of mice: the experimental animals Kunming mice, male, 10 weeks old, were randomly divided into two groups according to body weight, 30 mice in each group, 1 hour before hypoxia , The drug group and the control group were orally given 3mg / kg of yak blood deproteinized extract, and the same amount of starch, and the hypoxia tolerance of mice was observed by applying decompression and hypoxia method. The hypoxia altitude was 10,000 meters, the ascending speed was 15 meters per second, and the animal mortality was observed within 1 hour. Results: The survival rate of the mice in the medication group was 50%, and the survival rate of the control group was 18%. It can be seen that the deproteinized extract of yak blood can significantly improve the hypoxia tolerance of animals.

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Abstract

The invention refers to a deproteinized extraction of yak blood, the extracting method and the application. It is a physiological component, the molecular weight less than 5000 or 6000 Daltons; the purification process: collect yak blood on axenic condition, separate out blood serum, filter by Milipore ultrafilter, then sterilize and freeze out the ultrafiltrate. It can be used as drug, health food and food for curing diabetes and syndrome of diabetes, and for preventing and curing variou incomplete brain functions such as senile dementia, extracranial wound, brain coma, etc.

Description

technical field [0001] The present invention relates to a deproteinized extract extracted from the blood of yak (including young yak within six months of birth) and its extraction method and application. The invention belongs to the fields of biological pharmaceutical preparations, health food and food production methods and applications. Background technique [0002] In 1968, Parade et al. reported for the first time that the deproteinized extract from the blood of calves (specifically cattle) within six months of newborn not only has insulin-like effects, but also stimulates the uptake and utilization of oxygen by cells. At the same time, some scholars have noticed that the deproteinized extract of adult bovine blood also has the above biological activities, but its effect is far inferior to that of the deproteinized extract of calf blood within six months of the newborn. The significant difference in biological activity between the deproteinized extract of adult bovine b...

Claims

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Application Information

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IPC IPC(8): A61K35/14A61P3/10A61P9/10A61P25/28A61P43/00
Inventor 魏战增
Owner 魏战增
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