Exhibiting method of preparing antibody by antigen utilizing bacteriophage exhibiting technique

Abstract

The invention comprises, fusing exogenous antigen genes and filobactivirus III gene proteins and expressed onto the tail of filobactivirus, fusing antigen epitopes and filobactivirus III gene proteins and expressed onto the surface of filobactivirus, preparing antibodies taking filobactivirus with exogenous antigen and antigen epitopes as the immunogen. The invention claims:1)a method of preparing monoantibodies and polyantibodies based on filobactivirus showing technology;2) a method of preparing cTnI monoantibody and polyantibody based on filobactivirus showing technology showing antigen and antigen epitopes of human troponin; 3) a PIII filobactivirus showing carrier pFD5; 4) a filobactivirus showing fusion expressing carrier pFD5-cTnI;5) a PIII filobactivirus showing fusion expressing carrier PC89-cTnI[95??08];6)taking filobactivirus particles showing cTnI and cTnI[95í½108] as immunogens, immunizing new zealand white rabbit to get cTnI antibody.

Description

1. Technical field [0001] The invention belongs to the technical field of genetic engineering. This technology uses the principle of phage display technology to display the fusion of exogenous antigen gene and filamentous phage III gene protein on the tail of phage particle, and display the fusion of antigen epitope and filamentous phage VIII gene protein on the surface of filamentous phage particle. Antibodies are prepared by using phage particles displaying foreign antigens and antigenic epitopes as immunogens. 2. Background technology [0002] In the past 20 years, antibodies have been widely developed as in vitro diagnostic drugs in the fields of medicine and biology. In the past half century, detection technologies based on the principles of enzymatic reaction kinetics, chemiluminescence, ligand-receptor combination, etc., especially based on the three major protein labeling technologies (fluorescence, nuclides, enzymes) And the establishment of specific and sensitive...

AI Technical Summary

Problems solved by technology

However, for some rare antigens with small molecular weight and short half-life, such as certain hormones and their metabolites, certain tumor markers, etc., it brings difficulties to antigen purification and antibody preparation

Genetic engineering can effectively solve this problem. For example, the general expression system can solve the production and preparation of rare proteins, but it cannot solve the immunogenicity of small molecular peptides, because small molecular peptides for the purpose of preparing antibodies are expressed and purified. Finally, it still needs to be coupled with a macromolecular carrier, the coupling process is extremely cumbersome and complicated, and the coupling reaction is not easy to control

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