Bolengsu compound and its prepn, medicine composition and use

A technology of bolens and bolens, which is applied in the field of bolens compound and its preparation method, its pharmaceutical composition and application, and can solve the problems such as no medical reports

Inactive Publication Date: 2004-04-07
302 MILITARY HOSPITAL OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Cucurbitaceae plant Herpetospermum pedunculosum (Sex.) Baill. Boling

Method used

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  • Bolengsu compound and its prepn, medicine composition and use
  • Bolengsu compound and its prepn, medicine composition and use
  • Bolengsu compound and its prepn, medicine composition and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation of Cucurbitaceae plant Herpetospermum pedunculosum (Sex.) Baill.

[0049] Take 5 kg of the seeds of the medicinal material Boling melon seeds, crush them, soak them in 95% ethanol (5 times the amount), and extract the ethanol under reflux 3 times, each time for 1 hour, combine the extracts, concentrate until there is no alcohol smell, let stand to remove the oil layer, petroleum After removing residual oil with ether (one-time amount), ethyl acetate (one-time amount, one-time amount, one-time amount) extracted three times. Recover ethyl acetate to obtain dry powder. This part was subjected to repeated silica gel column chromatography, followed by dichloromethane: acetone 50:1, 30:1, 10:1, 5:1, 1:1 gradient elution, and collected 10:1, 5:1, 1:1 1 eluate, the crude product of wavelensin was obtained.

[0050] The crude boronsin was prepared by HPLC to obtain the boronsin (HPLC condition) with a purity of over 98%. The specific process is shown in ...

experiment example 1

[0054] In vivo anti-hepatitis virus effect of Experimental Example 1 Bolensin

[0055] 1.1 Reagents Lamivudine, British Glaxo Wellcome Co., Ltd. B022306.

[0056] a- 32 P-dCTP (Beijing Furui Biotechnology Engineering Company, batch number: 981206); gap translation kit (Promega company, batch number: 990426); duck hepatitis B virus is strongly positive for duck hepatitis B virus DNA (DHBV-DNA) Shanghai Shelduck serum (provided by the virus laboratory of the Institute of Pharmaceutical Biotechnology, Chinese Academy of Medical Sciences, stored at -70°C)

[0057] 1.2 Animals 1-day-old Beijing Wei duck was purchased from Beijing Nanyuan Duck Farm

[0058] 1.3 Methods 1-day-old Peking ducks were injected with DHBV-DNA-positive duck serum from Shanghai shelduck ducks through the tibial vein, 0.3ml each, and blood was collected 7 days after infection to separate the serum and detect the DHBVDNA content in the serum. After the duckling serum was tested positive for DHBV, the ducks ...

experiment example 2

[0070] Anti-hepatitis virus effect of experimental example 2 wave lentin

[0071] 2.1 Reagents

[0072] DMEM (product of Gibco, U.S.); fetal bovine serum (product of Gibco, U.S.); G-418 (product of Sigma, U.S.); HBeAg, HBsAg stationary phase radioimmunoassay kit (product of China Isotope Corporation, North Immunoreagent Research Institute);

[0073] 2.2.2.2.15 The cell is a human hepatoblastoma cell line transfected with the whole genome of HBV (Aayw subunit) and can secrete HBsAg, HBeAg, and HBV-DNA particles. It was constructed by the Mount Sinai Medical Center in the United States, and the Chinese Academy of Medical Sciences Medical Biotechnology Subculture in the virus room)

[0074] 2.3 Method Inoculate 24-well culture plate with 100,000 2.2.15 cells per milliliter, 1ml per well, 37℃, 5% CO 2 Incubate for 24 hours, add 2-fold dilution of the test solution below the non-toxic concentration, and the 5 dilutions are 4, 2, 1, 0.5, 0.25 mg / ml, 3 wells for each concentration,...

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Abstract

The present invention discloses the extractive of Herpetospermum pedunculo-sum (Sex.) Baill as one cucurbitaceous plant and Herpetospermum pedunculo-sum (Sex.) Baill essence, their preparation process, derivative, medicinal composite and medicinal use, especially in preparing medicines for resisting virus, resisting liver damage and resisting tumor. Cell expermiment and animal experiment proves the high activity and less toxicity of Herpetospermum pedunculo-sum (Sex.) Baill essence.

Description

technical field [0001] The present invention relates to Herpetospermumpedunculosum (Sex.) Baill. extract of cucurbit plant Herpetospermumpedunculosum (Sex.) Baill., a preparation method of Herpetospermumpedunculosum and its derivatives, and a pharmaceutical composition of the extract and its derivatives , the use of the extract of poleng, poleng and its derivatives and pharmaceutical compositions thereof as medicines, especially the use in the preparation of antiviral, anti-liver damage and anti-tumor medicaments. It has been confirmed by cell experiments and animal experiments that bolensin has the advantages of high activity and low toxicity. Background technique [0002] Chronic viral hepatitis is a common infectious disease that seriously endangers people's health. According to statistics, hepatitis B patients alone account for 5% of the world's population. The incidence rate of viral hepatitis in my country ranks first in the world, that is, about 80% of hepatitis pat...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61P1/16A61P31/12A61P35/00C07D307/80
Inventor 袁海龙李仙义
Owner 302 MILITARY HOSPITAL OF CHINA
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