Method of preparing catalase using extraction method

A technology of catalase and extraction method, which is applied in the field of preparing catalase to achieve the effects of convenient use, avoiding dye decomposition or dyeing defects, and saving water and energy

Inactive Publication Date: 2004-05-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Isolation and extraction of catalase from pig liver has not been reported in the literature of large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] After taking 200g of pig liver for beating, add 400g of water, process according to the above technical scheme, without concentration, to obtain the product catalase, the enzyme activity is ≥15000u / ml.

Embodiment 2

[0027] After taking 200g of pig liver for beating, add 400g of water, process according to the above-mentioned technical scheme, concentrate about 2 times, and obtain the product catalase, the enzyme activity of which is more than or equal to 30000u / ml.

Embodiment 3

[0029] After taking 200g of pig liver for beating, add 400g of water, process according to the above-mentioned technical scheme, and concentrate about 4 times to obtain the product catalase, the enzyme activity of which is more than or equal to 50000u / ml.

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PUM

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Abstract

A process for preparing the hydrogen peroxidase by extracting method includes immersing fresh pig liver or ox liver in water for dissolving the hydrogen peroxidase contained in them in the water, adding penicillin, centrifugal separation, adding chitin solution to flocculate other proteins, frame filtering with diatomite, ultrafiltering, adding NaCl for deposition, adding formaldehyde solution for sterilizing and clarifying. The product can be used to decompose the residual H2O2 in dyeing fabric. Its advantages are high extracting efficiency, less loss of enzyme activity and no environmental pollution.

Description

technical field [0001] The invention discloses a method for preparing catalase by an extraction method, which belongs to the technical field of enzyme products and relates to the preparation of catalase by an extraction method from animal liver. Background technique [0002] Catalase (CAT), also known as catalase, can be extracted from microbial fermentation or animal liver. Catalase is an enzyme preparation whose main function is to decompose hydrogen peroxide (commonly known as hydrogen peroxide). Catalase is widely used in textile industry, food industry and rubber and plastic industry. [0003] It is reported that one catalase molecule can decompose about 2,500,000 hydrogen peroxide molecules, and its decomposition ability is about 10 times that of iron ions. 6 times, so catalase is widely used in industry. Especially in the printing and dyeing industry, catalase is used to decompose excess H in the bleaching and dyeing process 2 o 2 , which can not only save operat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C12N9/08
Inventor 沈国强孙亮花慧姜文侯
Owner JIANGNAN UNIV
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