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Heavy PSME1 gene for pig to pass breast, and its prepn. method

A gene and genome technology, applied in the field of porcine weaning weight PSME1 gene and its preparation, can solve problems such as research blanks

Inactive Publication Date: 2004-05-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the research on porcine PSME1 gene at home and abroad is still blank

Method used

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  • Heavy PSME1 gene for pig to pass breast, and its prepn. method
  • Heavy PSME1 gene for pig to pass breast, and its prepn. method
  • Heavy PSME1 gene for pig to pass breast, and its prepn. method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] The distribution of PCR-RFLP-Sph I polymorphism in three pig breeds

[0117] (1) Primer sequence: 5'-TGAAAAGAAGAAGGGGGAAG-3' (forward, as shown in the sequence table SEQ ID: 3),

[0118] 5'-GTAAGCATTGCGGATCTCCA-3' (reverse, as shown in the sequence listing SEQ ID: 4)

[0119] (2) PCR amplification conditions: The total volume of PCR reaction is 20μl, including about 100ng of porcine genomic DNA, containing 1×buffer (Promega), 1.5mmol / L MgCl 2 , the final concentration of dNTP is 150 μmol / L, the final concentration of primer is 0.2 μmol / L, and 2U Taq DNA polymerase. Amplification program: 94°C for 4min, then 35 cycles of 94°C for 40s, 62°C for 45s, 72°C for 1min, and finally 72°C extension for 5min. PCR reaction products were detected by 2% agarose gel electrophoresis.

[0120] (3) RFLP detection conditions: Enzyme digestion reaction volume is 15μl, including 1.5μl of 1×buffer, 3~5μl of PCR product, 0.5μl (5U) of restriction endonuclease Sph I, make up 15...

Embodiment 2

[0130] We used the established PCR-RELP diagnostic method to detect the genotype of PSME1-RFLE-SphI on 190 DNA samples from a Large White herd.

[0131] The test results showed that among 190 individuals, there were 10 individuals with AA genotype, 62 individuals with AB genotype, and 118 individuals with BB genotype. In the association analysis with weaning weight, the results of the simple mean and standard deviation analysis of the traits among the genotypes are summarized in Table 4. The results of correlation analysis showed that the weaning weights of AA and AB genotype pigs were significantly different (P<0.01), and the weaning weights of AA and BB genotype pigs, and AB and BB genotype pigs were significantly different (P<0.05).

[0132] Table 3: Association analysis of different PSME1 gene SphI-RFLP genotypes and weaning weight

[0133] Genotype Genotypes Individual number N Weaning weight Weaning weight(kg)

[0134] Psme1-SphI AA 10 11.57 ± 1.51

[013...

Embodiment 3

[0141] The relationship between the genotypes of 108 Tongcheng pigs and the weaning weight of piglets was detected by the genetic diagnosis method as described above. The relationship between the genotype of Tongcheng pigs and the weaning weight of piglets is shown in Table 5.

[0142] Table 4 The relationship between the genotype of Tongcheng pigs and the weaning weight of piglets

[0143] Genotype Number of individuals Weaning weight (kg)

[0144] Psme1-SphI AA 2 10.69 ± 1.35

[0145] BB82 9.36±1.58

[0146]AB 24 10.21±1.40

[0147] P-value BB-AB 0.032

[0148] In this example, since only 2 pigs with AA type were detected, we ignored the AA type when doing correlation analysis, and re-tested the T-test only for the weaned pigs with BB-AB type. The results showed that the weaning weights of AB and BB pigs were significantly different, which was consistent with the previous results.

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Abstract

The present invention relates to the cloning and preparing process and application of sow ablactation gene PSME1. Its preparing steps include computer aided cloning, extracting RNA of sow spleen tissue, RACE, cloning RACE resultant, sequencing to obtain total length of cDNA sequence, designing primer, amplifying genomic DNA sequence, testing the RFLP polymorphism, analyzing the association between RFLP polymorphism and ablactation, and testing the SSCP polymorphism.

Description

technical field [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to a pig weaning weight PSME1 gene and a preparation method thereof Background technique [0002] In recent years, scholars at home and abroad have conducted a lot of research on the relationship between pig genetic markers and production performance. Appropriate genetic markers are of great benefit to carrying out marker-assisted selection, implementing early selection, improving the accuracy of selection and accelerating genetic progress. [0003] Weaning weight is an important indicator of sow fecundity, and it has a strong positive correlation with pig growth during finishing and slaughter weight. Generally speaking, piglets with significant weaning have better growth rate and higher feed remuneration, shorter pig raising period and lower feeding cost, and have high economic benefits. Weaning is serious, on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/04C12N15/11C12P19/34C12Q1/68
Inventor 李奎王彦芳余梅刘榜潘佩文熊统安樊斌赵书红
Owner HUAZHONG AGRI UNIV
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