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D-ribose fermentation process regulating method

A fermentation process and ribose technology, which is applied in the field of regulation of D-ribose fermentation process, can solve the problems of optimization and control that have not yet matured, and achieve the effect of enhanced repeatability and easy control

Inactive Publication Date: 2004-06-30
CHENGZHI LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no mature method for the optimal control of the D-ribose fermentation process of these strains.

Method used

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  • D-ribose fermentation process regulating method

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Experimental program
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Effect test

Embodiment 1

[0052] B. pumilus CGMCC0845 was used. In 350L seed medium (pH7.0) composed of sorbitol 2.0% (w / w), corn steep liquor 2.0%, yeast extract 0.1%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05% ), control the pressure of the seed tank to 1.0kg / cm 2 , rotating speed 200 rpm, culture temperature 36°C, after 16 hours of culture, insert glucose 16.0% (w / w), corn steep liquor 2.6%, yeast extract 0.1%, ammonium sulfate 0.7%, manganese sulfate 0.005%, calcium carbonate In 3200L fermentation medium (pH 7.0) with a composition of 2.2%, the stirring speed was controlled at 180 rpm, and the culture temperature was 36°C. In the early stage of fermentation for 0-18 hours, the pH is controlled at 6.5-7.0, and the dissolved oxygen is 20-25%. 18 hours before fermentation, glucose consumption is slow, mainly in the growth stage of bacteria. After 18 hours of fermentation, glucose consumption began to accelerate, D-ribose began to accumulate, an...

Embodiment 2

[0058] Bacillus subtilis (B. subtilis) CGMCC0846 was used. In a 350L seed medium composed of glucose 2.0% (w / w), yeast extract 0.3%, corn steep liquor 0.05%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05% (pH7.0) In the middle, control the pressure of the seed tank to 1.0kg / cm 2 , rotating speed 200 rev / min, culture temperature 36 ℃, after cultivating 18 hours, access is made up of glucose 16.0% (w / w), corn steep liquor 2.6%, ammonium sulfate 0.7%, manganese sulfate 0.005%, calcium carbonate 2.2%. 3200L fermentation medium (pH7.0). The stirring speed was controlled at 180 rpm, and the culture temperature was 36°C. In the early stage of fermentation for 0-18 hours, control at pH 6.5-7.0, dissolved oxygen 18-22%; in the middle and late stages of fermentation, control at pH 6.0-6.2, dissolved oxygen 20-25%, at 36°C, 0.60kg / cm 2 Culture under aeration and agitation. 18 hours before fermentation, glucose consumption is slow, ma...

Embodiment 3

[0064] Bacillus pumilus ATCC31093 was used. In 350L seed culture medium composed of sorbitol 2.0%, corn steep liquor 2.0%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, tyrosine and phenylalanine each 100 μg / ml (pH7.0) , control the pressure of the seed tank to 1.0kg / cm 2, rotating speed 200 rpm, culture temperature 36°C, after 16 hours of culture, insert glucose 16.0% (w / w), dry yeast 1.2%, ammonium sulfate 0.6%, manganese sulfate 0.005%, calcium carbonate 2.2%, tryptophan In the 3200L fermentation medium (pH7.0) that each 50 μ g / ml of acid, tyrosine and phenylalanine are formed, adopt the fermentation control condition identical with embodiment 1, in fermentation culture 33 hours, the glucose in the fermented liquid drops To 2.5~3.0%, control the sugar concentration in the fermented liquid in the range of 2.5~3.0%, start to feed sugar with the flow velocity of 120 liters / hour, the sugar concentration of feeding is 40%, and the volume is 550L. Af...

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Abstract

The invention relates to a D-ribose fermenting process optimized regulating and controlling method, a fermentation control method of making the control of dissolved oxygen and pH value as main line, and the control of key variables such as thallus growth quantity, substrate consuming rate and product generating rate as main content, and timely carrying out sugar supplement by fed-batch. The fermenting result in 50001 fermentation cylinders shows that: the method can raise D-ribose sugar-producing level by 20-34%, the fermenting period is shortened to 42-50 hours, the filling coefficient of the fermentation cylinder is raised from 79% to 80% above, and the fermenting process is easy to control and the repeatability is strengthened.

Description

(1) Technical field [0001] The invention relates to a method for regulating and controlling the D-ribose fermentation process. (2) Background technology [0002] The preparation method of D-ribose includes methods such as extraction and separation from natural substances, chemical synthesis and microbial fermentation. Extracting and separating D-ribose from natural products requires complicated extraction process and high manufacturing cost, which is not suitable for large-scale production. As far as the synthesis method is concerned, people have tried successively from D-arabinose (Gehrke and Aichner, 1927), D-gluconic acid (Steiger, 1936), D-glucose (Korczynski A et al.1979), L-glutamic acid Synthesize D-ribose with D-xylose (Lacourt Gadras, et al.1992). Before the 1980s, the industrial production of D-ribose was mainly carried out by chemical synthesis of glucose at home and abroad, that is, by oxidation and replacement of glucose , conversion, replacement, acidificatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02
Inventor 高润香李文濂梁是森王威
Owner CHENGZHI LIFE SCI CO LTD