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Use and preparation of collagen protein and collagen polypeptide

A collagen and collagen polypeptide technology, applied in the direction of fermentation, can solve the problems of inability to separate, long washing time, poor degreasing effect, etc., and achieve the effect of improving efficiency and reducing cost

Inactive Publication Date: 2004-12-08
MINGRANG BIOLOGICAL SCI & TECH SICHUAN PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, due to degreasing with degreasing enzyme or degreasing agent alone, the degreasing effect is not good, and the washing time is long. The produced product forms an emulsion with lipid in water and cannot be separated, which is not conducive to the use of collagen and collagen polypeptide products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Put 100g of the mechanically crushed alkali skin into a 500ml flask, add 400g of deionized water, stir and wash at room temperature twice until the pH value is about 9, and pour off the water. Add 400 g of deionized water and 0.6 g of alkaline lipase, stir in a water bath at 40° C. for 30 minutes, and pour off the water. Then add 1 gram of Pingpingjia, 1 gram of Tuwen-80, 0.5 gram of polyoxyethylene-21 stearyl ether and 400 g of deionized water, continue to react for 1 hour under the same conditions, and pour off the water. 100 g of deionized water was added, and the temperature was raised to 80° C., and treated at a constant temperature for 30 minutes for thermal degradation. Then lower the temperature to 40°C, add 0.3g trypsin, degrade for 3 hours, then use HCL to adjust the pH to about 6.5, add 0.6g microbial 1398 protease, degrade for 8 hours, then raise the temperature to 90°C, keep it for 20 minutes to inactivate the enzyme , and then filtered, the filtrate was d...

Embodiment 2

[0020] Put 100g of the mechanically crushed alkali skin into a 500ml flask, add 400g of deionized water, stir and wash at room temperature twice until the pH value is about 9, and pour off the water. Add 400 g of deionized water and 0.2 g of delipase, stir in a water bath at 40° C. for 30 minutes, and pour off the water. Then add 1 gram of Pingpingjia-OP, 2 grams of Tween-60, 0.5 gram of polyoxyethylene-21 stearyl ether and 400 g of deionized water. After continuing to react for 1 hour under the same conditions, pour off the water. 100 g of deionized water was added, and the temperature was raised to 80° C., and treated at a constant temperature for 30 minutes for thermal degradation. Then lower the temperature to 40°C, adjust the pH to about 2 with HCL, add 0.6g pepsin, degrade for 6 hours, then adjust the pH to about 6.5 with NaOH, add 0.2g neutral protease, degrade for 4 hours, and then heat up to 90°C, Incubate for 20 minutes to inactivate the enzyme, and then filter, and...

Embodiment 3

[0024] Determination of the properties of collagen and collagen polypeptide powder prepared by embodiment one and embodiment two

[0025] The average molecular weights of collagen and collagen peptides were about 20,000 and 2,000, respectively, and the average peptide chain lengths were 100-130 and 10-13, respectively, using a chromatograph (AKTA Explorer 100) produced by Pharmacia.

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PUM

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Abstract

This invention relates to preparations of collagen proteins and collagen peptides. It comprises: 1) mechanically pulverizing alkali leather or raw leather 100, washing at atmosphere and regulating pH 7- 10; 2) adding degreasing enzyme 0.1 - 1.2, stirring for 0.5 - 4 hours at 20 - 45 deg.C, washing; 3) adding surfactant 0.1 - 1.2, stirring for 0.5 - 4 hours at 20 -45 deg.C, washing; 4) adding active biological enzyme 0.1 - 0.8 at 30 - 65 deg.C, degrading for 0.5 - 12 hours; 5) heating to 75 - 90 deg.C for 15 - 30 minutes to inactivating enzyme; 6) filtering and distilling at 40 - 65 deg.C with pressure reduction; and 7) spraying to dry and obtain solid collagen proteins and collagen peptides powders. Owing to alkali degreasing enzyme and surfactant, leather degreasing rate will be up to 95% to produce easily soluble products with low cost and high productivity. The products can be used in skin nourishment and healthy food.

Description

technical field [0001] The present invention relates to a method for preparing collagen and collagen polypeptide and their application. Background technique [0002] Collagen is rich in 19 kinds of amino acids, including 7 kinds of amino acids necessary for the human body and 2 kinds of semi-essential amino acids for children, two more amino acids than human milk protein, and its protein content is 4 times higher than that of cow milk powder and goat milk powder , Collagen has been widely used in medicine, chemical industry, food and other fields. Compared with proteins and amino acids, polypeptides have more advantages. The absorption rate of polypeptides in the intestine is very high, and the absorption rate of some low-level peptides such as dipeptides and tripeptides is faster than that of amino acids of the same composition. Peptides have physiological functions such as low antigen, promoting lipid metabolism, lowering cholesterol, promoting mineral absorption, lowerin...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 史宗洁郭际王静
Owner MINGRANG BIOLOGICAL SCI & TECH SICHUAN PROV
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