Method for preparing rigid macromolecule medium in two kinds of hole types through dual emulsification

A double-emulsion and polymer technology, applied in the field of chromatographic medium preparation for the separation and purification of biological macromolecules, can solve the problems of easy fragmentation, limited application, irregular shape of particulate media, etc., and achieve improved mechanical strength, high dynamic adsorption capacity, The effect of large adsorption capacity
CN1563122AInactive Publication Date: 2005-01-12TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2005-01-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing rigid high-molecular medium with two kinds of pore forms by utilizing double emulsification, and said method includes the steps of selection of polymer monomer solution and pore-forming agent, formation of emulsion, polymerization reaction and washing, and it is characterized by that the monomer methyl glycidyl arylate, photoinitiator benzoin ethyl ether, emulsifier Span 80 and crosslinking agent ethylene dimethacrylate are formed into uniform mixed solution, the glycerine aqueous solution pore-forming agent is dispersed in mixed solution and formed into the emulsion liquor, said emulsion liquor can be formed into secondary emulsification in Tween 80 aqueou solution. The whole system can be undergone the process of UV photoinitiation suspension polymerization reaction under the condition of a certain rotating speed, after reaction the system is repeatedly washed by using water absolute ethyl alcohol so as to obtain the chromatographic separation medium with two pore form structures of super-large pore and diffusion pore.
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Description

technical field

[0001] The invention relates to a method for preparing two types of pore-type macromolecular rigid microsphere separation media by means of suspension polymerization induced by ultraviolet light using double-emulsion technology, which belongs to the preparation technology of chromatographic media for separation and purification of biological macromolecules. Background technique

[0002] The development of modern biomacromolecule separation and purification technology has promoted the development of life science, medicine and pharmaceutical industry. Liquid chromatography is an important tool for separation and purification of biomolecules, and the separation medium is particularly important. Therefore, the two types of pore-type media with excellent performance are important research topics in the direction of biological separation.

[0003] For faster and better separation, people have been trying to find good chromatographic media. When studying membranes ...

Claims

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