Method for preparing rigid macromolecule medium in two kinds of hole types through dual emulsification
A double-emulsion and polymer technology, applied in the field of chromatographic medium preparation for the separation and purification of biological macromolecules, can solve the problems of easy fragmentation, limited application, irregular shape of particulate media, etc., and achieve improved mechanical strength, high dynamic adsorption capacity, The effect of large adsorption capacity
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Embodiment 1
[0022]Weigh 0.08 g of benzoin ether and 1.5 g of Span 80 and dissolve them in 4.5 mL of glycidyl methacrylate (GMA), 3.0 mL of ethylene glycol dimethacrylate (EDMA); then add 3 mL of toluene and 2 mL of n-heptane well mixed. Add 8.5mL of 20% glycerin solution to the mixed solution, stir at 8000rpm for 5 minutes, the small droplets formed by its emulsification can be clearly seen under the microscope, then add the stirred emulsion to 60mL containing 1.8g In the aqueous solution of Tween 80, stir at 1600rpm for 1 minute, then transfer the entire mixed system to a reactor with electromagnetic stirring, and perform suspension polymerization under a 2kW ultraviolet lamp for 20 minutes. The reaction product was transferred to a PTFE bag, washed with water several times, air-dried under natural conditions, and then extracted with absolute ethanol for 24 hours to remove the organic porogen and emulsifier, and the above two types of pores were obtained after vacuum drying. Type micros...
Embodiment 2
[0024] Weigh 0.08 g of benzoin ether and 1.5 g of Span 80 and dissolve them in 4.5 mL of glycidyl methacrylate (GMA), 3.0 mL of ethylene glycol dimethacrylate (EDMA); then add 3 mL of toluene and 2 mL of n-heptane to mix uniform. Add 4.5mL of 20% glycerol solution to the mixed solution, stir for 5 minutes at 8000rpm, the emulsified droplets can be clearly seen under the microscope, then the stirred emulsion is added to the mixture containing 1.8 grams of Tween 80 60mL of water, stirred at 1600rpm for 1 minute, and then the entire mixed system was transferred to a reactor with electromagnetic stirring, and suspension polymerization was carried out under an ultraviolet lamp for 20 minutes. The reaction product was transferred to a Teflon gauze bag, washed with water, air-dried under natural conditions, and then extracted with absolute ethanol for 24 hours to remove organic porogens and emulsifiers, and vacuum-dried to obtain the above two types of micropores. 3.0 grams of spher...
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