Method for preparing rigid macromolecule medium in two kinds of hole types through dual emulsification

A double-emulsion and polymer technology, applied in the field of chromatographic medium preparation for the separation and purification of biological macromolecules, can solve the problems of easy fragmentation, limited application, irregular shape of particulate media, etc., and achieve improved mechanical strength, high dynamic adsorption capacity, The effect of large adsorption capacity

Inactive Publication Date: 2005-01-12
TIANJIN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

The experimental results show that the separation medium can greatly improve the mass transfer performance in the column, but due to the irregular shape of the particle medium, it is prone to breakage at high flow rates, which limits its application.

Method used

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  • Method for preparing rigid macromolecule medium in two kinds of hole types through dual emulsification
  • Method for preparing rigid macromolecule medium in two kinds of hole types through dual emulsification
  • Method for preparing rigid macromolecule medium in two kinds of hole types through dual emulsification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022]Weigh 0.08 g of benzoin ether and 1.5 g of Span 80 and dissolve them in 4.5 mL of glycidyl methacrylate (GMA), 3.0 mL of ethylene glycol dimethacrylate (EDMA); then add 3 mL of toluene and 2 mL of n-heptane well mixed. Add 8.5mL of 20% glycerin solution to the mixed solution, stir at 8000rpm for 5 minutes, the small droplets formed by its emulsification can be clearly seen under the microscope, then add the stirred emulsion to 60mL containing 1.8g In the aqueous solution of Tween 80, stir at 1600rpm for 1 minute, then transfer the entire mixed system to a reactor with electromagnetic stirring, and perform suspension polymerization under a 2kW ultraviolet lamp for 20 minutes. The reaction product was transferred to a PTFE bag, washed with water several times, air-dried under natural conditions, and then extracted with absolute ethanol for 24 hours to remove the organic porogen and emulsifier, and the above two types of pores were obtained after vacuum drying. Type micros...

Embodiment 2

[0024] Weigh 0.08 g of benzoin ether and 1.5 g of Span 80 and dissolve them in 4.5 mL of glycidyl methacrylate (GMA), 3.0 mL of ethylene glycol dimethacrylate (EDMA); then add 3 mL of toluene and 2 mL of n-heptane to mix uniform. Add 4.5mL of 20% glycerol solution to the mixed solution, stir for 5 minutes at 8000rpm, the emulsified droplets can be clearly seen under the microscope, then the stirred emulsion is added to the mixture containing 1.8 grams of Tween 80 60mL of water, stirred at 1600rpm for 1 minute, and then the entire mixed system was transferred to a reactor with electromagnetic stirring, and suspension polymerization was carried out under an ultraviolet lamp for 20 minutes. The reaction product was transferred to a Teflon gauze bag, washed with water, air-dried under natural conditions, and then extracted with absolute ethanol for 24 hours to remove organic porogens and emulsifiers, and vacuum-dried to obtain the above two types of micropores. 3.0 grams of spher...

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Abstract

The invention discloses a method for preparing rigid high-molecular medium with two kinds of pore forms by utilizing double emulsification, and said method includes the steps of selection of polymer monomer solution and pore-forming agent, formation of emulsion, polymerization reaction and washing, and it is characterized by that the monomer methyl glycidyl arylate, photoinitiator benzoin ethyl ether, emulsifier Span 80 and crosslinking agent ethylene dimethacrylate are formed into uniform mixed solution, the glycerine aqueous solution pore-forming agent is dispersed in mixed solution and formed into the emulsion liquor, said emulsion liquor can be formed into secondary emulsification in Tween 80 aqueou solution. The whole system can be undergone the process of UV photoinitiation suspension polymerization reaction under the condition of a certain rotating speed, after reaction the system is repeatedly washed by using water absolute ethyl alcohol so as to obtain the chromatographic separation medium with two pore form structures of super-large pore and diffusion pore.

Description

technical field [0001] The invention relates to a method for preparing two types of pore-type macromolecular rigid microsphere separation media by means of suspension polymerization induced by ultraviolet light using double-emulsion technology, which belongs to the preparation technology of chromatographic media for separation and purification of biological macromolecules. Background technique [0002] The development of modern biomacromolecule separation and purification technology has promoted the development of life science, medicine and pharmaceutical industry. Liquid chromatography is an important tool for separation and purification of biomolecules, and the separation medium is particularly important. Therefore, the two types of pore-type media with excellent performance are important research topics in the direction of biological separation. [0003] For faster and better separation, people have been trying to find good chromatographic media. When studying membranes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F2/00C08F2/48C08F220/16
Inventor 孙彦孙国勇史清洪董晓燕白姝
Owner TIANJIN UNIV
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