Heparin compound, and its preparing method and use

Abstract

The invention refers to a heparin compound and its preparation method and application. The heparin compound is a heparin zinc-potassium double salt demonstrated by the formula of R(ZnOH)aKb. The R represents the heparin radical and a is between 3 and 5, b between 1 and 3. The heparin compound can be made through the following preparation method: dissolve the sodium heparin into the water and contact with the cinnamene cation exchange resin to get the heparin solution; add an amount of zinc hydroxide and potassium hydroxide to the heparin solution, whisk and dry it and get the mentioned solid heparin compound. The heparin compound can be used as the anticoagulant agent as well as the main material of the anti thrombus medicine. The heparin zinc-potassium as the anticoagulant agent meets not only the requirements of not affecting the components' balance and stability of the blood samples to be tested but also the requirement of the solubility in the blood. When it is chosen as the main resource of the anti thrombus medicine, the heparin zinc-potassium releases slowly, has long effect of medicine and no toxicity and is good for the zinc-adding of the human body.

Description

technical field [0001] The invention relates to a heparin compound and its preparation method and application. Background technique [0002] In routine blood tests, blood samples must be anticoagulated with anticoagulants. At present, vacuum blood collection tubes with anticoagulants pre-prepared in test tubes are used. Heparin is a mucopolysaccharide containing sulfuric acid groups. It is a polymer substance with a molecular weight between 6000 and 15000. On average, each heparin molecule contains six sulfuric acid groups. It is widely found in animal lungs, livers, spleens, large intestines, etc. In human body tissue, it is mainly extracted from pig intestinal lining at present. Heparin can strengthen antithrombin to inactivate serine protease, so it has various anticoagulant effects such as preventing thrombin formation, antithrombin and preventing platelet aggregation. However, the solubility of heparin in the blood is very small and it is not easy to preserve, so it i...

AI Technical Summary

Problems solved by technology

However, the solubility of heparin in the blood is very small and it is not easy to preserve, so it is not suitable for clinical application in medical testing. Therefore, heparin is generally made into heparin salts that are easily soluble in blood to be suitable for clinical testing in medical testing. Heparin sodium and heparin are commonly used. lithium

However, heparin sodium interferes with the sodium ion test in the dielectric test in serum biochemical tests. Generally, it needs to be performed twice in the dielectric test; currently, heparin lithium is used to replace heparin sodium. Since the dielectric test in serum biochemical tests does not include lithium ions, therefore The test of the electrolyte in the serum can be completed at one time, which simplifies the operation procedure, but the activity of lithium ions is small, and it is easy to react to the Cl in the electrolyte - 、Na + , Ca 2+ 、K + 、H + Interference, affecting test quality

In order to overcome the above-mentioned problem of heparin sodium and heparin lithium interfering with the dielectric test in serum biochemical detection, some people consider manufacturing and using heparin zinc, and the zinc in heparin zinc is ZnOH + The form is combined with the sulfuric acid group on the heparin group through a coordination bond, and does not exchange with the ions in the dielectric, so it can maintain the balance of the components to be tested in the blood sample, so that the blood sample to be tested remains stable and has a long retention time without affecting Tests for various ions in dielectrics, but zinc heparin has low solubility in blood

There are foreign patent documents related to heparin lithium zinc double salt, but there is a shortage of heparin lithium zinc double salt. Because the solubility of lithium salt is slightly small, if the solubility of heparin lithium zinc double salt is to meet certain requirements, it is necessary to make heparin lithium zinc double salt The content of lithium ions in is enough, but too much lithium ions will affect the Cl in the electrolyte - 、Na + , Ca 2+ 、K + 、H + Interference, affecting test quality