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Chitosan glycyrrhizic acid nano particle and its preparing method

A technology of nanoparticles and chitosan, which is applied in pharmaceutical formulations, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., can solve problems such as no relevant literature reports, and achieve simple operation and stability. High, high encapsulation efficiency

Inactive Publication Date: 2005-03-02
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no relevant literature reports on the technology of chitosan and glycyrrhizic acid composite nanoparticles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Preparation of low solid content chitosan monoammonium glycyrrhizinate nanoparticles

[0033] Preparation of Chitosan Acidic Aqueous Solution

[0034] In a 500ml volumetric flask, add chitosan 0.3g, 36% acetic acid aqueous solution 0.84g, then add water to 300g. Stir at room temperature for 48 hours, and filter to obtain an aqueous chitosan-acetic acid solution with a concentration of 1.0 mg / ml.

[0035] Preparation of alkaline aqueous solution of monoammonium glycyrrhizinate

[0036] In a 50ml volumetric flask, add 30.0mg of monoammonium glycyrrhizinate, add 20.0g of water, heat and stir at 50°C for 24 hours to dissolve completely, add 0.12g of 25% ammonia water after cooling, then add water to 30.0g, stir and mix well Afterwards, an aqueous ammonium glycyrrhizinate salt solution with a concentration of 1.0 mg / ml was obtained.

[0037] Preparation of Chitosan Glycyrrhizic Acid Nanoparticles

[0038] Take 1.0 g of the ammonium glycyrrhizinate aqueous s...

Embodiment 2

[0038] Take 1.0 g of the ammonium glycyrrhizinate aqueous solution in a round-bottomed flask, add water to dilute to 10.0 g, stir for 10 minutes, then slowly add 10.0 g of chitosan aqueous solution dropwise, and continue stirring for 0.5 hours after the dropwise addition. A water dispersion system of blue-white opalescent chitosan glycyrrhizic acid nanoparticles was obtained, with an average particle diameter of 50nm, a drug loading capacity of 16.5%, and an embedding rate of 88.0%. Embodiment 2; Preparation of high solid content chitosan monoammonium glycyrrhizinate nanoparticles

[0039] Preparation of Chitosan Acidic Aqueous Solution

[0040] Same as example 1, except that the concentration of final chitosan is 5.0wt%, and the concentration of acetic acid is 1.0wt%.

[0041] Glycyrrhizinate monoammonium salt alkaline aqueous solution was prepared

[0042] Same as example 1, except that the concentration of prepared glycyrrhizic acid is 1.0wt%, and the concentration of amm...

Embodiment 3

[0045] Embodiment 3: Preparation of chitosan diammonium glycyrrhizinate nanoparticles

[0046] The preparation of chitosan acidic aqueous solution is the same as example 1.

[0047] Glycyrrhizinate diammonium salt alkaline aqueous solution was prepared

[0048] Same example 1, just replace monoammonium glycyrrhizinate with diammonium glycyrrhizinate.

[0049] Preparation of Chitosan Glycyrrhizic Acid Nanoparticles

[0050] Get in 10.0g chitosan aqueous solution round-bottomed flask, slowly add chitosan aqueous solution dropwise after the glycyrrhizic acid aqueous solution among the example 1 is diluted 10 times, dropwise finish and continue to stir for 0.5 hour, obtain blue opalescent chitosan glycyrrhizic acid The nanoparticle water dispersion system has an average particle diameter of 55nm, a drug loading capacity of 7.9%, and an embedding rate of 85.8%.

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PUM

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Abstract

The present invention relates to preparation process of nano chitosan-glycyrrhizic acid particle. Glycyrrhizic acid has the effects of resisting viral hepatitis and chronic hepatitis and destroying HIV cell in blood vessel, but available clinically applied glycyrrhizic acid preparations have the problem of hard absorption. The present invention prepares powdered nano chitosan-glycyrrhizic acid particle through mixing chitosan dissolved in acid aqua and glycyrrhizic acid dissolved in ammonia solution, synthesis under mild condition and freeze drying. The product of the present invention may be re-dispersed in water to form nano particle and has certain targeting and delayed releasing performance. Compared with available marketed oral preparation, the present invention has obviously raised intestinal absorption and bioavailability.

Description

technical field [0001] The invention is a method for preparing chitosan glycyrrhizinic acid nanoparticles by ion cross-linking reaction. Background technique [0002] Nanoparticles have many advantages in drug delivery, such as slow release of drugs, thereby prolonging the action time of drugs; achieving the purpose of targeted delivery of drugs; Or avoid adverse reactions; it can improve the stability of the drug, which is beneficial to storage; it is also possible to establish some new routes of administration, including local administration in vivo, mucosal absorption administration, and oral administration of polypeptides. At present, the carriers that can be used to prepare drug nanoparticles mainly include synthetic, biodegradable, high molecular polymers and natural macromolecular systems. Synthetic biodegradable polymers include: polyanhydride, polycaprolactone, polylactic acid, polyglycolic acid and their copolymers. Natural macromolecular systems include proteins...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/704A61K47/36A61P1/16A61P31/12
Inventor 郑永丽吴雁杨武利府寿宽汪长春胡建华沈锡中
Owner FUDAN UNIV
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