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Method of determining polysaccharide content in sea cucumbus

A technology of polysaccharide content and sea cucumber, applied in the field of determination of polysaccharide content in sea cucumber, achieves the effect of simple operation, high accuracy and good reproducibility

Inactive Publication Date: 2005-06-08
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the method for determining the content of polysaccharides in sea cucumbers has not been reported yet

Method used

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Embodiment Construction

[0006] Weigh 1 gram of pulverized plum flower ginseng (Thelenota ananas) sample, accurate to 0.0001 gram; put it into a 100ml beaker, add 40 grams of 3% (weight percentage concentration, the same below) KOH aqueous solution, and clean it with ultrasonic waves at 45°C React in the instrument for 2 hours; use glacial acetic acid to adjust the pH value within the range of 6.8-7.2; centrifuge at 10,000 rpm at 4°C for 25 minutes, take the supernatant, and put it into a 250ml centrifuge cup; slowly add 95% ethanol under stirring to make it The concentration of ethanol reached 60%, and stood overnight; centrifuged at 4500rpm for 30min, and discarded the supernatant; the precipitated polysaccharide was dissolved in 10g distilled water, and 85g of 95% ethanol was slowly added under stirring, and allowed to stand overnight; Suction filtration in a melting crucible, followed by washing and dehydration with absolute ethanol and acetone three times each; drying at 50°C to constant weight, c...

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Abstract

A method for measuring content of polysaccharide in sea cucumber, has the characteristics that the shattered sample of sea cucumber is added into water solution containing 3%-5% base which is 35-45 times of the mess of sample, then the solution is put into the super sonic wave washing instrument waiting for 1.5-2.5 hours under 40-50deg.C; use acid to adjust PH value to be neutral; the solution is centrifuged for 15-25 min by 8000-12000rpm and then get some clear liquid in which ethanol is added when being stirred to make the density of ethanol reach 55-65%; then after standing for a night; it is centrifuged for 20-30min by 4000-4500rpm and the clear liquid is abandoned and the sediment is dissolute by water in which ethanol is added into, making the density of ethanol reach 80-90%, after standing for a night; use bushing to filter and use orderly absolute ethyl alcohol and acetone to wash and anhydrate for three times; it is dried to have no change of mass and cooled to reach the room temperature, then be weighed. The invention uses secondary ethanol sediment to purify the sample and super sonic wave washing instrument as reactor, chooses bushing as mess analysis container.

Description

technical field [0001] The invention relates to a method for determining polysaccharide content in sea cucumbers. Background technique [0002] Sea cucumber (Holothutia) belongs to the class Holothurioidea of ​​the phylum Echinodermata. There are about 900 species in the world, and about 140 species in my country; about 40 species are edible in the world, and about 20 species in my country. In my country, sea cucumbers have always been regarded as a good adjunct to food. After the Ming Dynasty, sea cucumbers were included in herbal medicines and listed as tonics. Sea cucumber has a long history as a health food, and it is well known for its delicious taste and rich nutrition. In recent years, the efficacy of sea cucumber polysaccharides has attracted close attention from people at home and abroad. Pharmacological studies have also proved that it has a variety of medicinal effects, such as anticoagulant and blood fat reduction, inhibition of platelet aggregation, prevention...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N5/04
Inventor 王远红吕志华郭少媛
Owner OCEAN UNIV OF CHINA
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