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Blood pretreatment process for limulus reagent detection

A technology of blood treatment and Limulus reagent, which is applied in the field of blood pretreatment, can solve the problems of low endotoxin recovery rate, many supporting equipment, cumbersome operation, etc., and achieve the effects of low requirements, improved efficiency, and time saving

Inactive Publication Date: 2005-06-29
丁友玲
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Problems solved by technology

At present, the pretreatment methods used to remove interfering factors in blood mainly include dilution heating method, chloroform extraction method, perchloric acid method or new perchloric acid method, etc. The first two methods are due to the low recovery rate of endotoxin and the existence of false positives. , so it needs to be discussed in the determination; although the perchloric acid method or the new perchloric acid method has a better recovery rate of endotoxin, its operation is more cumbersome and there are many supporting equipment

Method used

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Experimental program
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Effect test

Embodiment Construction

[0006] The present invention is achieved like this: in the beaker that draws Triton X-100 stock solution 0.45ml to 250ml with precision pipette: take by weighing respectively potassium hydroxide 1.96g, two (2-hydroxyethyl) glycine Add 0.59g, Ethyleneimineploymer0.072g, calcium chloride 11.2g, and polybrene0.43g to the above 250ml beaker; add a small amount of water to dissolve and transfer to a 100ml volumetric flask, rinse the beaker with water for 2 to 3 times, and transfer the rinse solution to In the volumetric flask, add water to make it up to the mark, oscillate the volumetric flask to mix well, and then get the desired blood treatment solution; take 100 μL of plasma or serum, add 150 μL of blood treatment solution, mix well, and place in a constant temperature water bath at 37°C for 10 minutes; add Limulus reagent was used for experiments.

[0007] The invention provides a more precise, accurate and rapid new method for clinical diagnosis of Gram-negative bacterial infe...

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Abstract

It is a blood pre-process method used in horsefoot test, which comprises the following steps: to make the blood pre-process liquid processed by bent X-100 primary liquid, potassium hydroxide, bicine, Ethyleneimineploymer, calcii chloridum and polybrene; to mix it with the serum or blood plasma in the water of 37 degree for eight to fifteen minutes. This method can effectively removes the disturbance materials in the blood sample and reduces the disturbance in the action of the horsefoot and the inner toxin.

Description

technical field [0001] The invention relates to a blood pretreatment method for detecting a limulus reagent. Background technique [0002] Blood in the cardiovascular system is composed of tangible substances (red blood cells, white blood cells, platelets) and liquid plasma. Due to its complex composition, many of the substances, especially the plasma or serum of higher animals, have a certain reaction to Limulus reagent and endotoxin. Strong interference, so when detecting endotoxin in such samples, the interfering substances must be removed. At present, the pretreatment methods used to remove interfering factors in blood mainly include dilution heating method, chloroform extraction method, perchloric acid method or new perchloric acid method, etc. The first two methods are due to the low recovery rate of endotoxin and the existence of false positives. , so it needs to be discussed in the determination; although the perchloric acid method or the new perchloric acid method ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N33/50G01N33/579
Inventor 丁友玲
Owner 丁友玲
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