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Process for preparing microbiological polysaccharide Gellan gum

A microbial polysaccharide and gellan gum technology, which is applied in fermentation and other directions, can solve the problems of low product yield, high production cost, and high price, and achieve the effects of reasonable process design, reduced production cost, and high product yield

Active Publication Date: 2005-07-06
HEBEI HENBO BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only the American CP keclo company in the world has achieved industrial production, and its production and sales are in a monopoly position, and its production technology is difficult, so the price is expensive
Some colleges and universities in China have also tried to produce gellan gum with relatively simple production technology, but the effect has not been ideal
The patent No. is that 00125858.3 patent discloses a kind of production technology of new microbial polysaccharide gellan gum, but above-mentioned prior art exists production cost height, and product yield is low (the concentration of gellan gum crude polysaccharide is 1.3g / 100ml )Shortcomings

Method used

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Experimental program
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Embodiment Construction

[0030] The present invention will be further described below in conjunction with embodiment:

[0031] In the present embodiment, the production bacterial classification selects the sphingomonas paucimobilis sphingomonas paucimobilis bacterial classification of the genus Elodea (pseudomonaselodea) for use, and the production process comprises the following steps:

[0032] A, the original bacterial classification is made the slant bacterial classification;

[0033] B, the slant bacterial classification prepared in the A step is made seed liquid through expanding culture;

[0034] C, the seed liquid prepared in the B step is pressed seed liquid: fermented medium is that the inoculum size of 7%-10% inserts fermented medium and inserts and ferments, and wherein fermented medium comprises the following components (parts by weight):

[0035] Starch 2.8 Soybean Meal 0.2 NH 4 NO 3 0.1 MgSO 4 0.01

[0036] K 2 HPO 4 0.15 CaCO 3 0.05 water 96.6

[0037] The temperature of the fe...

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Abstract

The invention relates to a preparation method of microbe polysaccharide Gellan gum belonging to the microbe fermentation production field. The method comprises steps of preparing the slope strains, preparing the seed liquid, inoculating the seed liquid into the fermentation culture medium to ferment. The invention solves the problems of high production cost, low product yield (the density of the Gellan gum coarse polysaccharide is 1.3g / 100ml) exiting in known technology. The invention employs starch as the carbon source for the second level expanding culture medium and fermentation culture medium, and has advantages of low cost, high yield. The density of the Gellan gum coarse polysaccharide is 1.5-1.6g / 100ml when the fermentation is finished.

Description

technical field [0001] The invention relates to the field of microbial fermentation production, in particular to a preparation method of microbial polysaccharide gellan gum. Background technique [0002] Gellan gum is a kind of transparent gel prepared by aerobic fermentation of bacteria in the genus Pseudomonas elodea using modern bioengineering technology and a special refining technology. It has good thermal stability. stability. Less dosage, good taste retention, acid resistance, alkali resistance, biological enzyme resistance, and gel strength can be adjusted according to needs, widely used in food, medicine, fine chemical industry and other industries. At present, only the American CP keclo company has achieved industrial production in the world, and its production and sales are in a monopoly position, and its own production technology is difficult, so the price is expensive. Some colleges and universities in China have also tried to produce gellan gum with relativel...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 张禹张国佩张茶张少华
Owner HEBEI HENBO BIO TECH
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