Human marrow mesenchymal stem cell in vitro amplification method

A technology of bone marrow mesenchymal and in vitro expansion, which is applied in the field of regulating the proliferation of human bone marrow mesenchymal stem cells cultured in vitro, and can solve the problems of the influence of the proliferation of bone marrow mesenchymal stem cells and other issues.

Inactive Publication Date: 2005-10-05
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far there is no report on the effect of hypoxia on the proliferation of human bone marrow mesenchymal stem cells cultured in vitro

Method used

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  • Human marrow mesenchymal stem cell in vitro amplification method
  • Human marrow mesenchymal stem cell in vitro amplification method
  • Human marrow mesenchymal stem cell in vitro amplification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Isolation, cultivation and identification of human bone marrow mesenchymal stem cells

[0019] 1. Isolation and culture of human bone marrow mesenchymal stem cells

[0020] About 1 ml of femoral bone marrow was aseptically extracted from the fetus (6-month-old fetus induced by water bag, provided with the informed consent of the puerpera), transferred into a sterile centrifuge tube containing 3 ml of DMEM-LG medium (product of Hyclone Company), and centrifuged at 900 g for 5 min. Remove fat and supernatant, add 70% Percoll separation solution (Pharmacia company product) to the cell mass, and centrifuge at 900 g for 30 min. Take the cloudy cell layer at the interface and add DMEM-LG containing 20% ​​fetal bovine serum, pipette into a single cell suspension, count the nucleated cells, and divide the cells in 10 5 Inoculate in a 25ml culture flask at a density of 1 / ml, at 37°C, 5% CO 2 , saturated humidity CO 2 cultured in an incubator. After 24 hou...

Embodiment 2

[0023] Example 2 Proliferation-promoting Effect of Hypoxic Environment on Human Bone Marrow Mesenchymal Stem Cells

[0024] 1. Experimental grouping and determination of hypoxic conditions

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Abstract

This invention discloses a new method of myeloid stem cell external expansion. This invention adjusts the external myeloid stem cells through proper low oxide intensity, and maintains these cells with high hyperplasia state. The method of this invention adopts a controllable and non-trauma physical means and externally adjusts the hyperplasia of myeloid stem cells. It realizes the small amount of sampling, external expansion and large amount of obtaining in order to apply myeloid stem cells to cure many kinds of clinic trauma diseases, retrograde diseases, hereditary defects and etc, and it has good economic and social benefits.

Description

technical field [0001] The invention relates to a method for regulating cell proliferation, in particular to a new method for regulating the proliferation of human bone marrow mesenchymal stem cells cultured in vitro. Background technique [0002] Isolating and culturing target cells in vitro through tissue engineering technology for cell or tissue replacement therapy of related diseases in vivo has provided a new way for the treatment of various clinical diseases, and has attracted extensive attention and attention from scholars in recent years. However, the in vitro isolation and culture of various tissue cells is extremely difficult, which limits the development and promotion of this technology. Bone marrow mesenchymal stem cells have a wide range of sources and are more convenient to obtain. They can be differentiated into various tissue cells such as bone, cartilage, tendon, muscle, ligament, vascular endothelium, liver, nerve, skin and fat. Responses are weak and are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 李海生赵彤朱玲玲丁爱石赵惠卿范明
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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