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Method of high post extraction of acyl Gellan

A technology of high acyl gellan gum and extraction method, which is applied in the field of post-extraction of high acyl gellan gum, can solve the problems of high price and unreported issues, and achieve the effects of less consumption of raw materials, simple overall process and low energy consumption

Active Publication Date: 2005-10-26
HEBEI HENBO BIO TECH
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AI Technical Summary

Problems solved by technology

[0004] High acyl gellan gum, currently only produced by CPkeclo in the United States, is expensive and has not been reported in domestic patent literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] The post-extraction method of high acyl gellan gum comprises fermentation broth pretreatment and solid-liquid separation; each process step and process condition in the extraction method are:

[0028] (1) Fermentation broth pretreatment:

[0029] Adjust the pH value of the fermentation broth to 4.2, raise the temperature to 92°C, kill the enzyme and kill the bacteria, keep it for 20 minutes and then cool it down to room temperature;

[0030] (2) Flocculation and sedimentation, solid-liquid separation:

[0031] Add an electrolyte solution accounting for 2.5% of the volume of the fermentation broth to the pretreated fermentation broth, and react for 36 minutes. The electrolyte solution is an aqueous solution of alkali metal chloride, and 28% of the volume of the fermentation broth is added, and the concentration is more than 90% isopropanol After stirring evenly, solid-liquid separation;

[0032] (3) Drying and crushing:

[0033] The product prepared in step (2) was va...

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PUM

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Abstract

This invention relates to microorganism fermentation domain. It provides a post extraction method of acyl gellan. It consists of these steps: pretreatment of the fermentation solution, flocculent deposition; solid and liquid separation, dry, crush and so on. The invention resolves the problem of high acyl gellan gum, because it has been product by CPkeclo company of America, and its price is very high, characterized in that its cost is very low because of process is very simple, energy cost is low, material cost is low, and the quality reaches the FccIv standard of America.

Description

technical field [0001] The invention relates to the field of microbial fermentation production, in particular to a post-extraction method of high acyl gellan gum. Background technique [0002] Gellan gum is a polysaccharide gum obtained by extracting and refining carbohydrates after pure fermentation of Pseudomonas elodea. It is a fully transparent gel. There are two forms of gellan gum: one is natural, with acyl groups attached to the main chain of gellan gum, that is, high-acyl gellan gum; the other is low-acyl, where the acyl groups on the main chain of gellan gum are removed or Partial removal. [0003] High acyl gellan gum is soft, elastic, strong adhesion, not easy to lose water, good water holding performance, low dosage, adjustable elasticity, good compatibility, excellent thermal stability, acid resistance, enzymolysis resistance, etc. Widely used in food, medicine, daily chemical and other industries, it is an ideal substitute for gels such as carrageenan and al...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 张禹张国沛
Owner HEBEI HENBO BIO TECH
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