Method and device for transporting or binding-specific separation of electrically charged molecules

A molecular and metal ion technology, applied in measurement devices, biochemical equipment and methods, analytical materials, etc., can solve problems such as high cost and high cost of manufacturing microsensor chips

Inactive Publication Date: 2006-01-11
SIEMENS AG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Although this known method is feasible, the production of the microsensor chip is significantly more expensive and therefore more expensive due to the introduction of new polymer layers

Method used

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  • Method and device for transporting or binding-specific separation of electrically charged molecules
  • Method and device for transporting or binding-specific separation of electrically charged molecules
  • Method and device for transporting or binding-specific separation of electrically charged molecules

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Embodiment Construction

[0027] figure 1 The schematic structure of a general device for carrying out biochemical measurements is shown. 1 denotes a plate-shaped base made of, for example, silicon, on which a substrate made of, for example, silicon oxide (SiO 2 ) made of thin insulating layer 2. On the device are two measuring electrodes 20 and 30, which are preferably made of a noble metal, in particular gold. The entire measuring device is in contact with an aqueous solvent 15 .

[0028] There are negatively charged macromolecules in the aqueous solvent 15, which in figure 1 Indicated by the thread ball structure, and in the back Figure 5 Use 200, 200' to mark respectively. The negatively charged molecules are supposed to migrate towards the measuring electrodes 20 , 30 and are also referred to below as target molecules. In the case of DNA analysis the target molecule is the DNA to be examined. By means of capture molecules, which can be immobilized, for example, in the hydrogel layer 35 , t...

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Abstract

Transporting electrically charged molecules (A) in an aqueous solution, especially for operating a DNA sensor with a redox-cycling process between two measuring electrodes, is new. Transporting electrically charged molecules (A) in an aqueous solution, especially for operating a DNA sensor with a redox-cycling process between two measuring electrodes (ME) comprises (a) arranging, near ME, a metallic material (M) that is stable in aqueous solution and less resistant to oxidation than ME, and functions as a potential-stressable electrode; (b) applying a positive potential so that M is solubilized as positive ions; and (c) negatively charged molecules (A'), the target molecules, are transported in the opposite direction so that they concentrated at ME. Independent claims are also included for: (1) similar method for binding-specific separation of (A) in aqueous solution where the solution contains metal ions, a negative potential is applied to ME so that the metal ions are deposited (as metal) and (A') that are near ME and have sufficiently low binding energy are transported away from ME; and (2) device for performing the new methods.

Description

technical field [0001] The invention relates to a method for the migration and bond-specific separation of charged molecules in aqueous solvents, in particular when operating a DNA sensor with a redox cycle process between two measuring electrodes. Furthermore, the invention relates to a related device. Background technique [0002] In many molecular biology methods, the migration of charged particles in an electric field plays an important role. Here, the diffusion velocity v of charged particles in the fluid medium is proportional to the electric field strength E and ion charge Q, and inversely proportional to the particle radius r and the viscosity η of the suspension. The velocity v is given by: [0003] v=QE / 6πrη (1) [0004] For example, biomolecules that differ with respect to their size and / or charge, such as mainly proteins and DNA, are separated from each other in electrophoresis. In certain forms of electrophoretic separation (eg isoelectron focusing) the pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543C12Q1/68
CPCG01N27/3277G01N27/48G01N33/5438
Inventor 海克·巴拉格沃尔特·冈布雷赫特曼弗雷德·斯坦泽尔
Owner SIEMENS AG
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