Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and device for transporting or binding-specific separation of electrically charged molecules

A molecular and metal ion technology, applied in measurement devices, biochemical equipment and methods, analytical materials, etc., can solve problems such as high cost and high cost of manufacturing microsensor chips

Inactive Publication Date: 2006-01-11
SIEMENS AG
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although this known method is feasible, the production of the microsensor chip is significantly more expensive and therefore more expensive due to the introduction of new polymer layers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and device for transporting or binding-specific separation of electrically charged molecules
  • Method and device for transporting or binding-specific separation of electrically charged molecules
  • Method and device for transporting or binding-specific separation of electrically charged molecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] figure 1 The schematic structure of a general device for carrying out biochemical measurements is shown. 1 denotes a plate-shaped base made of, for example, silicon, on which a substrate made of, for example, silicon oxide (SiO 2 ) made of thin insulating layer 2. On the device are two measuring electrodes 20 and 30, which are preferably made of a noble metal, in particular gold. The entire measuring device is in contact with an aqueous solvent 15 .

[0028] There are negatively charged macromolecules in the aqueous solvent 15, which in figure 1 Indicated by the thread ball structure, and in the back Figure 5 Use 200, 200' to mark respectively. The negatively charged molecules are supposed to migrate towards the measuring electrodes 20 , 30 and are also referred to below as target molecules. In the case of DNA analysis the target molecule is the DNA to be examined. By means of capture molecules, which can be immobilized, for example, in the hydrogel layer 35 , t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Transporting electrically charged molecules (A) in an aqueous solution, especially for operating a DNA sensor with a redox-cycling process between two measuring electrodes, is new. Transporting electrically charged molecules (A) in an aqueous solution, especially for operating a DNA sensor with a redox-cycling process between two measuring electrodes (ME) comprises (a) arranging, near ME, a metallic material (M) that is stable in aqueous solution and less resistant to oxidation than ME, and functions as a potential-stressable electrode; (b) applying a positive potential so that M is solubilized as positive ions; and (c) negatively charged molecules (A'), the target molecules, are transported in the opposite direction so that they concentrated at ME. Independent claims are also included for: (1) similar method for binding-specific separation of (A) in aqueous solution where the solution contains metal ions, a negative potential is applied to ME so that the metal ions are deposited (as metal) and (A') that are near ME and have sufficiently low binding energy are transported away from ME; and (2) device for performing the new methods.

Description

technical field [0001] The invention relates to a method for the migration and bond-specific separation of charged molecules in aqueous solvents, in particular when operating a DNA sensor with a redox cycle process between two measuring electrodes. Furthermore, the invention relates to a related device. Background technique [0002] In many molecular biology methods, the migration of charged particles in an electric field plays an important role. Here, the diffusion velocity v of charged particles in the fluid medium is proportional to the electric field strength E and ion charge Q, and inversely proportional to the particle radius r and the viscosity η of the suspension. The velocity v is given by: [0003] v=QE / 6πrη (1) [0004] For example, biomolecules that differ with respect to their size and / or charge, such as mainly proteins and DNA, are separated from each other in electrophoresis. In certain forms of electrophoretic separation (eg isoelectron focusing) the pres...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543C12Q1/68
CPCG01N27/3277G01N27/48G01N33/5438
Inventor 海克·巴拉格沃尔特·冈布雷赫特曼弗雷德·斯坦泽尔
Owner SIEMENS AG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com