Method for preparing extract product of paeoniflorin

A technology of extract and paeoniflorin, which is applied in the field of medicine, can solve the problems of unstable quality and low content of active ingredients, and achieve the effects of stable quality, good curative effect, and easy operation of the preparation process

Active Publication Date: 2006-02-01
NANJING HAILING TRADITIONAL CHINESE MEDICINE RES CO LTD
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The content of active ingredients in Radix Paeoniae Rubra Injection currently used clinically is low, and the quality is not stable enough

Method used

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  • Method for preparing extract product of paeoniflorin
  • Method for preparing extract product of paeoniflorin
  • Method for preparing extract product of paeoniflorin

Examples

Experimental program
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preparation example Construction

[0017] Preparation of reference solution:

[0018] Accurately weigh an appropriate amount of the reference substance of paeoniflorin that has been dried in a phosphorus pentoxide decompression dryer for 36 hours, and add methanol to make a solution containing 0.5 mg per 1 ml.

[0019] Preparation of extract test solution:

[0020] Precisely weigh 16.16mg and 12.48mg of the extract respectively, put them in a 25ml volumetric flask, add methanol to dissolve and dilute to the mark, shake well to obtain Test 1 and Test 2.

[0021] Determination method: Precisely draw 2 μl of the reference solution, the solution of the test product 1, and the solution of the test product 2, respectively, inject 2 μl into the liquid chromatograph, and measure. figure 1 , figure 2 , image 3 .

[0022] The following examples are intended to further illustrate the invention without limiting it in any way.

Embodiment 1

[0024] Take 10kg of red peony medicinal material (origin: Heilongjiang), crush it into coarse powder, and put it into the extraction tank; add 60L and 50L of 70% ethanol for reflux extraction twice, each time for 1.5h, after the extract is filtered by heat, reduce Pressure concentrated, centrifuged, applied to a D101 macroporous adsorption resin column, fully eluted with water until the effluent was clear and no reducing sugar reaction, and then eluted with 20% ethanol, collected the eluate, and recovered ethanol under reduced pressure. Polyamide column, washed with water, concentrated under reduced pressure, and dried to obtain white powder. Measured by HPLC method: the content of paeoniflorin in the red peony root extract obtained in the above experiment is 95%.

Embodiment 2

[0026] Take 100kg of red peony medicinal material (origin: Heilongjiang), crush it into coarse powder, and put it into the extraction tank; add 800L and 600L of 50% ethanol respectively for reflux extraction twice, 1.5h each time, after the extract is filtered by heat, put it in Cool, concentrate under reduced pressure, centrifuge, apply to D401 macroporous adsorption resin column, fully elute with water until the effluent is clear and apply to polyamide column, wash with water, concentrate under reduced pressure, and dry to obtain white powder. Measured by HPLC method: the content of paeoniflorin in the red peony root extract obtained in the above experiment is 94.5%.

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Abstract

A process for preparing the high-purity paeoniflorin used to prepare the peony injection for treating cardiovascular and cerebrovascular diseases includes such steps as pulverizing red peony root, hot reflux extracting in alcohol 4-5 time, centrifugal separating, adsorbing by macroreticular resin column, water washing, eluting with alcohol, collecting the eluting liquid, concentrating, adsorbing by polyamide column, water eluting, concentrating and drying.

Description

technical field [0001] The invention relates to a preparation method of paeoniflorin, an effective part of traditional Chinese medicine red peony for treating cerebral thrombosis and other cardiovascular and cerebrovascular diseases, and belongs to the field of medicine. Background technique [0002] Paeonia suffruticosa is the dry root of Paeonia Lactiflora Pall. or Paeonia veilchii Lyncn. It is widely distributed in Hebei, Liaoning, Jilin, Heilongjiang, Shaanxi, Gansu and other places; it is bitter in nature, slightly cold, and returns to the liver meridian; its main effects are to clear away heat and cool blood, and to relieve pain. It is used for spots caused by warm poison, hematemesis, swelling and pain in the eyes, liver depression and hypochondriac pain, etc. Pharmacological studies in recent years have shown that red peony has many pharmacological effects such as protecting the heart, increasing coronary blood flow, antithrombotic and atherosclerosis, sedation and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/65A61P9/10
Inventor 张现涛李新岗严军何箐旋钱淑芸贾树田
Owner NANJING HAILING TRADITIONAL CHINESE MEDICINE RES CO LTD
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