Method for separating lentinan

A technology of lentinan and lentinan, which is applied in the field of extracting active ingredients of traditional Chinese medicine, can solve the problems of complicated process, increased cost, and consumption of ethanol, and achieve the effects of shortening the process, reducing production costs, and improving product quality

Inactive Publication Date: 2006-05-03
杨勇杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The extraction process of lentinan and lentinan is relatively complicated at present, and the commonly used extraction solvent is ethanol. The ethanol used to precipitate lentinan and lentinan is usually 2 to 4 times that of the extraction solution, so that the polysaccharide can be precipitated
However, the purity of the polysaccharide extracted in this way is not high, and a series of follow-up treatments are still required to make the polysaccharide content reach 90%, which greatly increases the cost and makes the price of lentinan high for a long time
In recent years, many people have improved the extraction method of lentinan and lentinan. There are nearly 30 patent applications related to lentinan in China, but none of them can get rid of the step of ethanol precipitation in the end. This extraction step not only A large amount of ethanol is consumed, and the content of lentinan obtained is not high, which makes the follow-up process complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Extraction of Lentinan from Lentinus edodes Fruiting Body

[0013] Dried shiitake mushrooms are crushed and passed through a No. 2 sieve. Weigh 200 grams of this powder, add 10 times the amount of water to extract at 90 degrees for 2 hours, repeat 2 times. The two extracts were combined and concentrated under reduced pressure to 400 ml. Add 80 grams of polyethylene glycol (PEG6000), stir well to dissolve the polyethylene glycol completely. Leave overnight for complete precipitation. The precipitate was obtained by centrifugation, and the precipitate was extracted with 200 ml of 95% ethanol under reflux for 2 hours, and repeated twice. The extracted precipitate was dried at a low temperature of 50° C. to obtain 6.2 grams of lentinan, a white powdery solid, with a polysaccharide content of 94%.

Embodiment 2

[0015] Extracting Lentinan from Lentinus stalk

[0016] Dried shiitake mushroom stalks are crushed and passed through a No. 2 sieve. Weigh 200 grams of this powder, add 10 times the amount of water to extract at 90 degrees for 2 hours, repeat 2 times. The two extracts were combined and concentrated to 500 ml by ultrafiltration using a membrane with a molecular weight cut-off of 5000. Add 80 grams of polyethylene glycol (PEG10000), stir well to dissolve the polyethylene glycol completely. Leave overnight for complete precipitation. The precipitate was obtained by centrifugation, and the precipitate was extracted with 200 ml of 60% ethanol under reflux for 2 hours, and repeated twice. The extracted precipitate was dried at a low temperature of 55° C. to obtain 5.1 grams of lentinan, a white powdery solid with a polysaccharide content of 92%.

Embodiment 3

[0018] Extraction of Lentinus polysaccharide from Lentinus edodes Fermentation Broth

[0019] Take 5000 ml of Lentinus edodes fermented liquid, boil and centrifuge to obtain the supernatant. Concentrate the supernatant to 500 ml, add 60 grams of polyethylene glycol (PEG20000), stir well to dissolve the polyethylene glycol completely. Leave overnight for complete precipitation. The precipitate was obtained by centrifugation, and the precipitate was refluxed and extracted with 200 ml of absolute ethanol for 2 hours, and repeated twice. The extracted precipitate is dissolved in heated water to remove insoluble matter, and freeze-dried to obtain 0.5 g of lentinan as a white powdery solid with a polysaccharide content of 95%.

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Abstract

The invention discloses a new method to separate lentinan and cartinellin amylose, which comprises: drying and breaking one or more of fruiting body, mycelium, handle and fermentation liquid of mushroom, adding water, heating, extracting; filtering the insolubles, condensing the liquid, adding carbowax, stirring to dissolve the carbowax; placing for a while; cleaning the separated deposition with organic solvent. This method just uses carbowax of 1/4~1/10 to alcohol, cuts process greatly, reduces cost, and the extract content more than 90%.

Description

technical field [0001] The present invention is an improvement to the existing extraction process of Lentinus polysaccharides and Lentinus polysaccharides, and discloses a new method for separating Lentinus polysaccharides and Lentinus polysaccharides. Any one or any two or more of them are used as raw materials for extraction, which relates to the technical field of extracting active ingredients of traditional Chinese medicine. Background technique [0002] Shiitake mushroom is a traditional Chinese medicinal material in my country, which has the effect of nourishing the liver and replenishing qi. Lentinan is one of the main active components of Lentinus edodes. It has anti-tumor, anti-virus, and therapeutic effects on hepatitis B, and is widely used. In 1985, Japan first successfully developed lentinan injection to treat gastric cancer. Then someone extracted polysaccharides from the submerged fermentation broth of shiitake mushrooms, and named it "lentinan" to distingui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 杨勇杰张萍姜瑞芝
Owner 杨勇杰
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