Probiotic composite enzyme production method
A production method and technology of probiotics, applied in the field of microbial fermentation in the biological field, can solve the problems of unsatisfactory, low shelf life, and high cost, and achieve the effects of low cost, long shelf life, and extended shelf life
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Embodiment 1
[0022] Concentrated probiotic compound enzyme raw materials were purchased from Japan Enzyme World Corporation and Japan Shimamoto Microbe Industry Co., Ltd.
[0023] 1. Mixing of concentrated probiotic compound enzymes and auxiliary materials:
[0024] Put 320kg of starch (accounting for 34.19% of the total weight of the raw material mixture) and 250kg of glucose (accounting for 26.71% of the total weight of the raw material mixture) into a micro mixer, and stir for 48 minutes at a temperature of 20°C. 146kg (accounting for 15.60% of the total weight of the raw material mixture) of concentrated probiotic compound enzyme raw material was added at 24°C, stirred for 42 minutes, and the relative humidity was 35%. Add 19.8kg (accounting for 2.1% of raw material mixture gross weight) ferment fruit juice, temperature 25 ℃, stir 28 minutes. Add 47.2kg (accounting for 5.04% of the total weight of the raw material mixture) functional factor stachyose, then add 153kg (accounting for 16...
Embodiment 2
[0031] The source of raw materials is the same as in Example 1.
[0032] 1. Preparation of fermentation raw material mixture:
[0033] Put 680kg of starch (accounting for 32.91% of the total weight of the raw material mixture) and 540kg of glucose (accounting for 26.14% of the total weight of the raw material mixture) into a micro mixer, and stir for 45 minutes at a temperature of 18°C. 340kg (accounting for 16.46% of the total weight of the raw material mixture) of concentrated probiotic compound enzyme raw material was added at 22°C, stirred for 38 minutes, and the relative humidity was 36%. Add 82.4kg (accounting for 3.99% of raw material mixture gross weight) ferment fruit juice, temperature 26 ℃, stirring time 27 minutes. Add 24.72kg (accounting for 1.20% of the total weight of the raw material mixture) of functional factor stachyose and 98.88kg (accounting for 4.79% of the total weight of the raw material mixture) of functional factor fructooligosaccharide. Then add 30...
Embodiment 3
[0040] The source of raw materials is the same as in Example 1.
[0041] 1. Preparation of fermentation raw materials:
[0042]Put 430kg of starch (accounting for 32.38% of the total weight of the raw material mixture) and 315kg of glucose (accounting for 23.72% of the total weight of the raw material mixture) into an electric mixer, and stir for 40 minutes at a temperature of 20°C. 195kg (accounting for 14.68% of the total weight of the raw material mixture) of concentrated probiotic compound enzyme raw material was added at 23°C, stirred for 45 minutes, and the relative humidity was 36%. Add 66.5kg (accounting for 5% of raw material mixture gross weight) ferment fruit juice, temperature 27 ℃, stir 28 minutes. Add 90.5kg (accounting for 6.81% of the total weight of the raw material mixture) of functional factor stachyose. Then add 231kg (accounting for 17.39% of the total weight of the raw material mixture) concentrated probiotic compound enzyme raw material, and continue...
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