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Method for producing recombinant interleukin for animal

A technology of interleukin and production method, which is applied in the field of production of recombinant interleukin for animals, can solve the problem of low activity, achieve the effect of enhancing activity, low production cost, and changing the way of existence

Active Publication Date: 2006-05-03
DALIAN SANYI ANIMAL MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the technical problem of low activity existing in the prior art, and provides a production method of recombinant interleukin for animals with high purity and strong activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0011] Prepare crude recombinant interleukin with existing technology, the specific method is as follows:

[0012] 1. According to various animal interleukin gene sequences published on Genbank, genomic RNA was extracted from ConA-stimulated chicken spleen lymphocytes, from mitogen-stimulated duck spleen lymphocytes, and genomes were extracted from pig liver and dog blood DNA;

[0013] 2. According to the interleukin sequence published on Genbank, use Oligo software to design a pair of oligonucleotide primers, and carry out molecular modification with restriction endonucleases at the 5′ ends of the upstream and downstream primers;

[0014] 3. If the extracted genome is RNA, use RT-PCR to amplify the interleukin gene; if the extracted genome is DNA, use PCR to amplify the interleukin gene;

[0015] 4. Sequence analysis of the target gene obtained by RT-PCR or PCR amplification;

[0016] 5. Clone the interleukin gene into the expression vector pBV220, and transform it into Esc...

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Abstract

The invention discloses a preparation method for animal recombined interleukin, which comprises: on prior base, condensing, separating, purifying, preparing and degerming with filtration membrane to coarse product. This product not only needs low cost and avoids carrying exogenous virus, but also has 25% protein yield during whole process, improves purity, and increases activity.

Description

Technical field: [0001] The invention relates to a production method of interleukin, in particular to a production method of recombinant interleukin for animals with high purity and strong activity. Background technique: [0002] Interleukin (interleukin, IL) refers to a class of cytokines produced by leukocytes or other somatic cells that can regulate and mediate between leukocytes. Interleukins can not only be used as immune adjuvants together with vaccines to significantly improve the immune protection effect of vaccines, but also can be used as therapeutic drugs in combination with some antiviral drugs to significantly improve the efficacy of drugs. In the past, the production method of interleukin was made from animal blood, which was not only costly, but also had the potential risk of carrying foreign viruses and other pathogenic microorganisms in the product. In order to overcome the disadvantages brought about by traditional production methods, people have used gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C12N15/24C12N15/70C12P21/02
Inventor 江国托
Owner DALIAN SANYI ANIMAL MEDICINE CO LTD