33 results about "Interleukin Gene" patented technology

The invention relates to a grass carp interleukin 1 beta gene and protein and a recombinant expression method thereof. The invention provides the grass carp interleukin 1 beta gene and the grass carp interleukin 1 beta protein coded by the gene. The recombinant expression method of the invention adopts homology cloning technology to clone and obtain the cDNA complete sequence of the grass carp interleukin 1 beta gene, uses the mature peptide sequence of the grass carp interleukin 1 beta gene to construct a prokaryotic expression vector and screens out a recombinant expression system used for efficiently expressing the grass carp interleukin 1 beta gene. The recombinant expression method of the invention comprises the following steps: 1. performing gene cloning; 2. constructing and screening the recombinant expression vector; and 3. expressing the recombinant protein, and separating and purifying the expression products. The beneficial effect of the invention is that the technical scheme of the invention can be used to perform the industrialized production of the recombinant interleukin 1 beta, and the recombinant interleukin 1 beta can be used in the theoretical research of the immunologic mechanism of fish.

The invention discloses an agent box for detecting female osteoporosis impressionable heredity risks. The agent comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting synchronously number rs1800795 SNP site on encode interleukins 6 gene, number rs2073618 SNP site on bone protection element TNFRSF11B gene, number rs1800629 SNP site on tumor necrosis factor-Alpha, number rs1544410 and rs731236 SNP site on vitamin D receptor gene, and general component for detecting fluorescent definite quantity PCR etc.. The agent box of the invention assesses female osteoporosis impressionable heredity risks by detecting synchronously mononucleotide polymorphism site gene type correlative closely to osteoporosis impressionable heredity risks.

The invention relates to immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404). The compound is obtained by the fermentation and separation of microorganism Bacillussimplex DR-834. As shown by numerous tests, the compound can be used for preparing drugs for enhancing the immune function of animals such aquatic animal fish. A crude fermentation product of the Bacillussimplex DR-834 containing cyclic tetrapeptide can be used as feedstuff. By using the proline-glycocoll cyclic tetrapeptide, the mRNA (Messenger RNA) expression level of interleukin 1-beta gene and nitric oxide synthase in blood is obviously increased compared with contrast, and the immunity of aquatic animals can be obviously improved. The result of a virus attacking test indicates that the cyclic tetrapeptide is remarkable in immune protection. In test groups, the average death rate is 26.67%, and the rate of a contrast group is 73.33% and the relative immune protection rate of the contrast group is 63.63%.

An anti-infectious immunopotentiator for animals is prepared through cloning the interleukin-6 gene of Chenghua pig, configuring its eukaryon expression plasmid VPIL-6, preparing chitosan nanoparticles from deacetyl chitosan and using said chitosan nanoparticles for molecular packaging of plasmid VPIL-6. It can be used to immunize the experimental animal by intramuscular injection or oral taking.

The present invention provides methods and tests that allow for the establishment of personalized weight-management programs for an individual based upon the individual's genotype in the glutathione S-transferase pi gene and / or the interleukin-6 gene. Methods are disclosed for determining the individual's genotype, which may be used to select an appropriate therapeutic / dietary program or lifestyle recommendation. Such a personalized weight-management program will have obvious benefits (e.g., yield better results in terms of weight loss and weight maintenance) over traditional weight-management programs that do not take into account genetic information.

The invention relates to the field of gene expression and in particular relates to an efficiently-expressed porcine interleukin-2 gene. The nucleotide sequence of the porcine interleukin-2 gene is expressed as a sequence 2; the gene of the sequence 2 is cloned to a recombinant plasmid acquired by an eukaryotic expression vector; the expression protein of the porcine interleukin-2 gene plays a role in obviously enhancing the immunity of an inactivated vaccine of porcine reproductive and respiratory syndrome; a pIL-2 protein gene is expressed by an eukaryotic plasmid pMVAX<0>-pIL-2 containing the sequence 2 in a sequence table, so that the limitations of low expression quantity and poor activity in the process of expressing the pIL-2 via a pMVAX<0> vector are broken, and the expression quantity is improved by 5.7 folds; and pig experiments prove that the expression activity of the expression protein pIL-2 of the gene in the sequence 2 in the sequence table is high, and the response of humoral immunity and cellular immunity of a pathogenicity PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) inactivated vaccine can be obviously improved, so that the expression protein pIL-2 is specially used for preventing diseases caused by high pathogenicity PRRSV and reducing financial loss of pig farms.

An interleukin-15 (IL-15) gene modified natural killing cell strain for immunotherapy of tumor is prepared through inserting the cDNA coding region of IL-15 in pcDNA3 eucaryotic expression carrier, configuring secretion-type recombinant eucaryotic expression carrier pc DNA3 / IL-15, using liposom to transfecte cell NK-92, adding Geneticin (G418) to screening culture medium, screening, limited dilution to cloned cells, detecting activity, choosing positive cell clones, and amplification.

The invention provides a modified recombinant human interleukin-12 (rhIL-12) gene and also provides a construction method of a rhIL-12 expression system in CHO (Chinese Hamster Ovary) cells. According to the method provided by the invention, a genetic engineering means is utilized to artificially synthesize recombinant genes of recombinant human interleukin-12; and the recombinant gene sequence is inserted into a vector and is transformed into a host cell, and a high-efficiency expression rhIL-12 eukaryotic cell line is obtained.

The invention provides a method for performing quantitative determination to immunity-relating cytokine in peripheral blood, the method can be applied for specifically detect the cytokine gene expression (IL-2) from 2ml of peripheral blood, and no any stimulation is needed before detection. The method can detect the cytokine gene expression under physiologic status, thus can be used for determining early stage fatigue, preventing decrease of body immunity function caused by exercises of excess sport load. The invention also provides a corresponding detection kit.

The present invention relates to the antificial synthesis of human interleukin-10 gene sequence and E. Coli containing the sequence. The present invention features that 35 sites of human interleukin-10 are codon substituted into E. Coli preference codon; pTRX-hIL-10 expression plasmid is constituted on the basis to obtain stable and efficient expression in E. Coli; and throgh separation and purification, hIL-10 recombinant protein with high purity and high activity is prepared. The present invention realizes in vitro expression of human interleukin-10 and this sets the foundation of producing the medicine with the matter.

The present invention discloses a cDNA of interleukin IL-12p40 gene, the nucleic acid sequence of which is shown in SEQ ID NO.1. The coded protein of the perch interleukin IL-12p40 gene is also disclosed, its amino acid sequence is shown in SEQ ID NO.2, and the present invention further discloses an expression vector comprising the cDNA. As well as the application of the coding protein or recombinant protein of the perch interleukin IL-12p40 gene in the preparation of medicines with antibacterial and fish immunity enhancing effects.

The invention relates to a kit for detecting the polymorphism of an interleukin 28B (IL28B) gene by utilizing a fluorescence PCR (Polymerase Chain Reaction) technology. The kit is characterized in that three primers are adopted in a single reaction tube to augment sequences of CC type and TT type of an rs12979860 gene, and the CC type and the TT type of the gene are detected by two MGB fluorescence probes with different wavelengths. The kit is simple in operation and accurate and reliable in detection result, can be applied to detection of the polymorphism of the IL28B rs12979860 gene of a patient with clinical hepatitis C and further predicts the treatment effect of antiviral drugs.

The invention belongs to the biology technical field, relating an antineoplastic agent taking IL-23 gene or a cell introduced to the IL-23 gene as effective ingredient. The invention comprises the antineoplastic agent taking the following substances as the effective ingredient: a virus representing the IL-23 gene or non-virus carrier, a tumor cell introduced the IL-23 gene losing fission ability and an immune cell introduced the IL-23 gene. The antineolastic agent of the invention can be used independently or be combined with the treatment methods of operation, actinotheraphy and medical treatment for improving the cell immune response of organism, thereby achieving the aim of decreasing tumor volume, preventing transfer and preventing the recurrence of the tumor.