33 results about "Interleukin Gene" patented technology

The invention relates to a grass carp interleukin 1 beta gene and protein and a recombinant expression method thereof. The invention provides the grass carp interleukin 1 beta gene and the grass carp interleukin 1 beta protein coded by the gene. The recombinant expression method of the invention adopts homology cloning technology to clone and obtain the cDNA complete sequence of the grass carp interleukin 1 beta gene, uses the mature peptide sequence of the grass carp interleukin 1 beta gene to construct a prokaryotic expression vector and screens out a recombinant expression system used for efficiently expressing the grass carp interleukin 1 beta gene. The recombinant expression method of the invention comprises the following steps: 1. performing gene cloning; 2. constructing and screening the recombinant expression vector; and 3. expressing the recombinant protein, and separating and purifying the expression products. The beneficial effect of the invention is that the technical scheme of the invention can be used to perform the industrialized production of the recombinant interleukin 1 beta, and the recombinant interleukin 1 beta can be used in the theoretical research of the immunologic mechanism of fish.

The invention discloses an agent box for detecting female osteoporosis impressionable heredity risks. The agent comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting synchronously number rs1800795 SNP site on encode interleukins 6 gene, number rs2073618 SNP site on bone protection element TNFRSF11B gene, number rs1800629 SNP site on tumor necrosis factor-Alpha, number rs1544410 and rs731236 SNP site on vitamin D receptor gene, and general component for detecting fluorescent definite quantity PCR etc.. The agent box of the invention assesses female osteoporosis impressionable heredity risks by detecting synchronously mononucleotide polymorphism site gene type correlative closely to osteoporosis impressionable heredity risks.

The present invention relates to a gene and a protein of grass carp interleukin 10 and a recombinant expression method thereof. The present invention provides a gene sequence of grass carp interleukin 10 and a grass carp interleukin 10 protein sequence coded by the gene sequence, and puts forward a prokaryotic recombinant expression method of the grass carp interleukin 10. A homologous cloning technology is employed to obtain a cDNA complete sequence of the grass carp interleukin 10 gene; a mature peptide sequence of the grass carp interleukin 10 gene is employed to construct prokaryotic expression vectors; and screening is carried out to obtain a prokaryotic expression system for efficient expression of the grass carp interleukin 10 gene. The method comprises specific steps of: 1. gene cloning; 2. construction of the recombinant expression vector; and 3. expression of the recombinant protein, and separation and purification of expression products. The invention has beneficial effect that the technical scheme of the invention can realize industrialized production of the recombinant grass carp interleukin 10, thereby applying the recombinant grass carp interleukin 10 to theoretical study on the mechanism of immunity of fish.

The invention relates to a chicken Eimeria acervulina immunoregulation type DNA vaccine, which belongs to the technical field of biological veterinary medicines. The molecular biology technology is used for connecting lactate dehydrogenase genes of schizont antigen of the first generation of chicken Eimeria acervulina and chIL-2 genes in series to construct a fusion expressing vaccine pVAX-LDH-IL-2. The vaccine comprises a plurality of T cell immune response elements, thus preventing and curing chicken coccidiosis effectively.

The invention relates to a recombinant fowlpox virus, in particular to a recombinant fowlpox virus which co-expresses a major protective antigen gene HA of an H5 subtype avian influenza virus and a chicken interleukin 2 gene as well as a construction method and applications of the recombinant fowlpox virus. The recombinant fowlpox virus rFPV-AIH5 / IL2 co-expresses the HA gene of the H5 subtype avian influenza virus and the chicken interleukin 2 gene and has CGMCC NO. of 2831. The fowlpox virus rFPV-AIH5 / IL2 can be applied into the preparation of H5 subtype avian influenza vaccine, and compared with recombinant fowlpox virus vaccines which expresses HA gene singly, the obtained vaccine can remarkably improve the immune protection level and lower the toxin expelling level of the H5 subtype avian influenza virus after infecting chickens.

The invention relates to an Eimeria maxima DNA vaccine of chicken, belonging to the field of biological veterinary drugs. A gene fragment Em8 of an antigen EmTFP250 at the spore stage of the Eimeria maxima of the chicken is cloned by utilizing the molecular biology technology, and the fragment has higher antigen index and the centralized T cell epitope. The gene fragment is connected with a chicken interleukin-2 (chIL-2) gene in series, thereby constructing a vaccine expression vector pVAX-Em8-IL-2. The vaccine contains a plurality of T cell immune response elements and has the advantages of high safety, long maintenance time in vivo, simple preparation, good thermal stability, convenient storage and transport, time-saving and effort-saving use, and the like. Experiments prove that the immune regulatory DNA vaccine pVAX-Em8-IL-2 has good immune protection effect on the anti-Eimeria maxima infection.

The invention belongs to the technical field of microorganisms, and particularly relates to an application of pseudoalteromonas fish killing in fish and shrimp culture. The strain is preserved in the China Center for Type Culture Collection in Wuhan on November 12, 2020, and the preservation number is CCTCC NO: M 2020731. The bacterial strain has broad-spectrum anti-vibrio activity, has strong antagonistic activity on Aeromonas salmonicida, can enhance expression of fish lysozyme, tumor necrosis factors and cell interleukin genes after being orally taken, can significantly improve the immune level of aquaculture animal body fluid, and in addition, the strain can also significantly increase the length of intestinal microvilli of aquaculture animals, has a trend of increasing muscle thickness and villi length, is beneficial to improving the intestinal function of the aquaculture animals, realizes the synergistic effect of antagonizing pathogenic bacteria, immunizing and promoting the intestinal function, and more effectively prevents and controls bacterial diseases in aquaculture.

The present invention relates to a transformed lymphokine activated killer cell (I-LAK cell) useful in the treatment of cancer or virus infection into which a recombinant vector carrying IL-2 gene to which it is operably linked is introduced. Also it relates to a pharmaceutical composition for treating cancer or virus infection disease comprising a sufficient amount of I-LAK cell to elicit an immune response.

An anti-disease preparation for animals is prepared through cloning the interleukin-4 and -6 gene of pig, fusing them, configuring the eukaryon expression plasmid VPIl 46 of fusion gene, preparing the chitosan nanoparticles by ion exchange method, dissolving the plasmid in the solution of tripolyphosphate and the deacetyl chitosan solution in acetic acid, and proportional mixing. It can be used to immunize the experimental animal by oral application.

A chicken's interleukin 18 as the immunopotentiator of fowls' vaccine is the aqueous solution of the eukaryon expression plasmid, which is a recombinant carrier composed of the p CI as the eukaryon expression carrier and interleukin 18 genes. It has high bioactivity and low cost.

The invention relates to immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404). The compound is obtained by the fermentation and separation of microorganism Bacillussimplex DR-834. As shown by numerous tests, the compound can be used for preparing drugs for enhancing the immune function of animals such aquatic animal fish. A crude fermentation product of the Bacillussimplex DR-834 containing cyclic tetrapeptide can be used as feedstuff. By using the proline-glycocoll cyclic tetrapeptide, the mRNA (Messenger RNA) expression level of interleukin 1-beta gene and nitric oxide synthase in blood is obviously increased compared with contrast, and the immunity of aquatic animals can be obviously improved. The result of a virus attacking test indicates that the cyclic tetrapeptide is remarkable in immune protection. In test groups, the average death rate is 26.67%, and the rate of a contrast group is 73.33% and the relative immune protection rate of the contrast group is 63.63%.

An anti-infectious nano-class molecular preparation for animals is prepared through fusing the interleukin-4 gene of pig with the interleukin-6 gene of pig, configuring its eukaryon expression plasmid VPIL 46, preparing chitosan nanoparticles by ion exchange method, dissolving said plasmid in the solution of tripolyphosphate and deacetyl chitosan solution in acetate acid, and proportional mixing. It can be used to immunize the experimental animal by intramuscular injection.

An anti-infectious immunopotentiator for animals is prepared through cloning the interleukin-6 gene of Chenghua pig, configuring its eukaryon expression plasmid VPIL-6, preparing chitosan nanoparticles from deacetyl chitosan and using said chitosan nanoparticles for molecular packaging of plasmid VPIL-6. It can be used to immunize the experimental animal by intramuscular injection or oral taking.

The present invention provides methods and tests that allow for the establishment of personalized weight-management programs for an individual based upon the individual's genotype in the glutathione S-transferase pi gene and / or the interleukin-6 gene. Methods are disclosed for determining the individual's genotype, which may be used to select an appropriate therapeutic / dietary program or lifestyle recommendation. Such a personalized weight-management program will have obvious benefits (e.g., yield better results in terms of weight loss and weight maintenance) over traditional weight-management programs that do not take into account genetic information.

The invention relates to a vaccine for preventing and treating scars and a preparation method thereof. The vaccine is formed by fusing an encoded human interlenkin 10 (hIL-10) gene, a polypeptide CBD gene specifically combined with collagen, and a eukaryotic expression vector pcDNA3.1(+) on the gene level. As proved by Balb / c mouse wound healing model testing, the vaccine can be used for continuously inducing synthetic secretion of hIL-10-CBD by a mouse in a certain period of time. A secreted hIL-10-CBD protein can be specifically combined with collagen generated by cells in a wound healing process, the vaccine quantity, toxic and side effects and treatment cost can be reduced, and the pharmacological action can be brought into play specifically and effectively at scar positions, so that fibrosis caused by excessive deposition of extracellular matrixes is prevented, and the curative effects of preventing and treating the formation of scars and improving scar appearances are achieved. The vaccine is formed by fusing human interlenkin 10 (hIL-10) with a CBD polypeptide which is specifically combined with collagen in a gene engineering measure on the gene level, and fusing with the eukaryotic expression vector pcDNA3.1(+) on the gene level.

The invention discloses a lateolabrax japonicus interleukin IL-12p40 gene and the application thereof. The lateolabrax japonicus interleukin IL-12p40 gene has the nucleic acid sequence shown as SEQ IDNO. The invention further discloses encoded protein of the lateolabrax japonicus interleukin IL-12p40 gene. The encoded protein has the amino acid sequence shown as SEQ ID NO.2. The invention furtherdiscloses an expression vector comprising cDNA and the application of the encoded protein or recombinant protein of the lateolabrax japonicus interleukin IL-12p40 gene to preparation of medicines with antibacterial and fish immunity enhancing efficacies.

The invention relates to the field of gene expression and in particular relates to an efficiently-expressed porcine interleukin-2 gene. The nucleotide sequence of the porcine interleukin-2 gene is expressed as a sequence 2; the gene of the sequence 2 is cloned to a recombinant plasmid acquired by an eukaryotic expression vector; the expression protein of the porcine interleukin-2 gene plays a role in obviously enhancing the immunity of an inactivated vaccine of porcine reproductive and respiratory syndrome; a pIL-2 protein gene is expressed by an eukaryotic plasmid pMVAX<0>-pIL-2 containing the sequence 2 in a sequence table, so that the limitations of low expression quantity and poor activity in the process of expressing the pIL-2 via a pMVAX<0> vector are broken, and the expression quantity is improved by 5.7 folds; and pig experiments prove that the expression activity of the expression protein pIL-2 of the gene in the sequence 2 in the sequence table is high, and the response of humoral immunity and cellular immunity of a pathogenicity PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) inactivated vaccine can be obviously improved, so that the expression protein pIL-2 is specially used for preventing diseases caused by high pathogenicity PRRSV and reducing financial loss of pig farms.

Oral cavity wound healing occurs in an environment that sustains ongoing physical trauma and is rich in bacteria. Patients undergoing cleft palate repair have a high degree of wound healing complications, such as oronasal fistula (ONF) formation. Following hard palate injury, ONF was created that demonstrated little change in pro-regenerative monocytes LY6Clo monocytes; however, there were increased M2 macrophages observed. Delivery of FTY720 nanofiber scaffolds following hard palate injury prevented ONF formation, allowed complete wound healing and was associated with increased LY6Clo monocytes and pro-regenerative M2 macrophages. Evaluation of interleukin gene expression revealed reduction in pro-inflammatory IL1 and IL6 and increased expression of pro-regenerative IL10 with FTY720 nanofiber delivery. The ability of FTY720 scaffolds to increase LY6Clo monocytes, increase M2 macrophages and alter the interleukin expression during hard palate mucosal healing demonstrates the ability of a FTY720-based autotherapy to improve oral cavity wound healing.

The invention provides a probe for detecting interleukin-3 gene polymorphism. The sequence of the probe is as shown in SEQ ID NO.: 1, or two ends of the sequence of SEQ ID NO.: 1 are modified by groups. The invention also provides a primer for detecting the polymorphism of the interleukin-3 gene. The sequence of the primer is shown as SEQ ID NO.: 2 (primer 1) and SEQ ID NO.: 3 (primer 2). When the probe and the primer for detecting the interleukin-3 gene polymorphism are used for detecting the interleukin-3 gene polymorphism, the probe and the primer have the advantages of high sensitivity, good specificity, rapidness, high-throughput detection and the like.

A method for evaluating an effect of an agent to be tested on alopecia, using a transgenic mouse to which an interleukin-18 gene under control of a keratinocyte promoter is introduced, or a method for evaluating an effect of an agent to be tested on skin, using the transgenic mouse, wherein the following (A) and / or (B) are used as an evaluation indicator. (A) spontaneous dermatitis (B) dermal inflammation at a time of a first administration of a phlogogenous material Using the transgenic mouse, the effect of an agent to be tested on dermatitis for which autoimmune dermatitis is a typical example and / or alopecia can be examined, and an agent for preventing / treating dermatitis or alopecia can be provided.

The invention provides a method for performing quantitative determination to immunity-relating cytokine in peripheral blood, the method can be applied for specifically detect the cytokine gene expression (IL-2) from 2ml of peripheral blood, and no any stimulation is needed before detection. The method can detect the cytokine gene expression under physiologic status, thus can be used for determining early stage fatigue, preventing decrease of body immunity function caused by exercises of excess sport load. The invention also provides a corresponding detection kit.

The present invention relates to the antificial synthesis of human interleukin-10 gene sequence and E. Coli containing the sequence. The present invention features that 35 sites of human interleukin-10 are codon substituted into E. Coli preference codon; pTRX-hIL-10 expression plasmid is constituted on the basis to obtain stable and efficient expression in E. Coli; and throgh separation and purification, hIL-10 recombinant protein with high purity and high activity is prepared. The present invention realizes in vitro expression of human interleukin-10 and this sets the foundation of producing the medicine with the matter.

The present invention discloses a cDNA of interleukin IL-12p40 gene, the nucleic acid sequence of which is shown in SEQ ID NO.1. The coded protein of the perch interleukin IL-12p40 gene is also disclosed, its amino acid sequence is shown in SEQ ID NO.2, and the present invention further discloses an expression vector comprising the cDNA. As well as the application of the coding protein or recombinant protein of the perch interleukin IL-12p40 gene in the preparation of medicines with antibacterial and fish immunity enhancing effects.

This invention discloses and claims a class of indole and benzo(b)thiophene derivatives for use in treating allergy, asthma, rhinitis, dermatitis, B-cell lymphomas, tumors and diseases associated with bacterial, rhinovirus or respiratory syncytial virus (RSV) infections. The compounds of this invention are defined by the Formula (I):wherein X, Y, Z, R1, R2 and R3 are as defined in the specification; or a pharmaceutically acceptable salt thereof. In preferred embodiments of this invention it is also disclosed and claimed that the compounds of this invention are capable of modulating T helper (Th) cells, Th1 / Th2, and thereby capable of inhibiting the transcription of interleukin-4 (IL-4) message, IL-4 release or IL-4 production.

The invention discloses a primer composition for detecting the expression level of a cynoglossus semilaevis interleukin IL-6 gene. The composition consists of a primer group 1 and a primer group 2; the primer group 1 is used for cynoglossus semilaevis IL-6 gene detection, and the primer group 2 is used for beta-actin reference gene detection; the primer group 1 is composed of primers as shown in a sequence SEQ ID NO. 1 and a sequence SEQ ID NO. 2; and the primer group 2 is composed of primers as shown in a sequence SEQ ID NO.3 and a sequence SEQ ID NO.4. According to the primer composition designed by the invention, specific amplification of the cynoglossus semilaevis IL-6 gene can be realized, amplification and melting curves of fluorescent quantitative PCR only have independent peaks, and the specificity is strong. The IL-6 gene expression level in a sample is quantified through a real-time fluorescence quantification method established by the invention, and the detection sensitivity is very high.

The invention relates to a kit for detecting the polymorphism of an interleukin 28B (IL28B) gene by utilizing a fluorescence PCR (Polymerase Chain Reaction) technology. The kit is characterized in that three primers are adopted in a single reaction tube to augment sequences of CC type and TT type of an rs12979860 gene, and the CC type and the TT type of the gene are detected by two MGB fluorescence probes with different wavelengths. The kit is simple in operation and accurate and reliable in detection result, can be applied to detection of the polymorphism of the IL28B rs12979860 gene of a patient with clinical hepatitis C and further predicts the treatment effect of antiviral drugs.

The invention discloses a molecular marker related to a bacterial septicemia resistant character of clarias fuscus. The molecular marker is located in an exon region of a CDS region of an interleukin 4 gene of the clarias suddenbergii, is related to antibacterial septicemia traits of the clarias suddenbergii, and can be applied to disease-resistant molecular marker-assisted breeding of the clarias suddenbergii, so that clarias suddenbergii individuals and families with relatively high disease resistance are cultured, and the breeding efficiency of the clarias suddenbergii is improved. And sustainable and healthy development of the silurus meridionalis breeding industry is promoted.

The invention belongs to the biology technical field, relating an antineoplastic agent taking IL-23 gene or a cell introduced to the IL-23 gene as effective ingredient. The invention comprises the antineoplastic agent taking the following substances as the effective ingredient: a virus representing the IL-23 gene or non-virus carrier, a tumor cell introduced the IL-23 gene losing fission ability and an immune cell introduced the IL-23 gene. The antineolastic agent of the invention can be used independently or be combined with the treatment methods of operation, actinotheraphy and medical treatment for improving the cell immune response of organism, thereby achieving the aim of decreasing tumor volume, preventing transfer and preventing the recurrence of the tumor.