Grass carp interleukin 1 beta gene and protein and recombinant expression method thereof

Abstract

The invention relates to a grass carp interleukin 1 beta gene and protein and a recombinant expression method thereof. The invention provides the grass carp interleukin 1 beta gene and the grass carp interleukin 1 beta protein coded by the gene. The recombinant expression method of the invention adopts homology cloning technology to clone and obtain the cDNA complete sequence of the grass carp interleukin 1 beta gene, uses the mature peptide sequence of the grass carp interleukin 1 beta gene to construct a prokaryotic expression vector and screens out a recombinant expression system used for efficiently expressing the grass carp interleukin 1 beta gene. The recombinant expression method of the invention comprises the following steps: 1. performing gene cloning; 2. constructing and screening the recombinant expression vector; and 3. expressing the recombinant protein, and separating and purifying the expression products. The beneficial effect of the invention is that the technical scheme of the invention can be used to perform the industrialized production of the recombinant interleukin 1 beta, and the recombinant interleukin 1 beta can be used in the theoretical research of the immunologic mechanism of fish.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a technology for recombinant expression and purification of grass carp interleukin-1β gene. Background technique [0002] Cytokines are a kind of non-antibody and non-complement produced by various cells, which can regulate cell growth and differentiation, regulate immune function, and participate in inflammation and wound healing. Hormone-like (trace and high-efficiency) soluble small molecule polypeptide substances. Interleukin-1β (IL-1β, Interleukin-1β) is one of the members of the interleukin-1 cytokine family. In addition to IL-1β, the family also includes interleukin-1α, interleukin-1 receptor antagonists (IL-1Ra) and interleukin-18 and some newly discovered members. Interleukin-1β is an important inflammatory mediator and immune factor, which plays a wide range of regulatory roles in the body's immune response, such as activating the activity of T cells, B cells a...

AI Technical Summary

Problems solved by technology

The blind and disorderly frequent use of various veterinary drugs and antibiotics not only has no obvious effect on the prevention and treatment of diseases, but also seriously damages the natural ecological environment due to the massive use of these drugs, and the drug residues directly threaten human health. Therefore, starting from the immune defense factors of grass carp itself to study its disease resistance mechanism and formulate new disease control measures has become an urgent problem to be solved.

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