Immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404) and application

A C14H20N4O4, proline-based technology, applied in cyclic peptides, applications, cyclic peptide components, etc., can solve problems such as serious side effects, pathogenic bacteria resistance, residues, etc., to improve quality and food safety, improve disease resistance, The effect of reducing the cost of farming

Inactive Publication Date: 2011-08-17
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While medicinal feed additives have significant effects, they also have adverse effects such as residue, toxicity, and "three causes" (teratogenic, carcinogenic, and mutagenic), and the side effects after use are also very serious. Long-term use can also cause pathogenic bacteria to produce drug resistance

Method used

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  • Immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404) and application
  • Immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404) and application
  • Immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404) and application

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Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: bacillus simplex ( Bacillus simplex ) DR-834 isolation and identification

[0038] The source of the strain was collected from the soil of Huashi Gorge in Yushu Prefecture, Qinghai Province. The strain number DR-834 was obtained by isolation and purification, which was identified as Bacillus simplex ( Bacillus simplex ). The strain is a microorganism announced by the Ministry of Agriculture of my country that can be used in farmed animals. Culture characteristics of strain DR-834.

[0039] Strain DR-834 was cultured in ordinary nutrient agar medium for 24 hours, and obvious colonies could be seen. The colonies were colorless, translucent, raised, round, with fuzzy edges, and the surface was smooth and shiny; after 48 hours in ordinary broth , turbid, without precipitation. The bacteria are Gram-positive, aerobic, motile, and spore-forming. Various physiological and biochemical characteristics of strain DR-834 are shown in Table 1.

[0040] The iso...

Embodiment 2

[0042] Embodiment 2: bacillus simplex ( Bacillus simplex ) Fermentation of DR-834, tracking and separation of active ingredients

[0043] (1) Materials and methods

[0044] 1. Instrument

[0045] Glass chromatography column: 8cm×100 cm; X-6 micro melting point apparatus: Beijing Tektronix Instrument Co., Ltd.; Perkin Elmer 240C elemental analyzer; time-of-flight mass spectrometer, HP company of the United States; Nicolet AVATAR 360 FT-IR infrared spectrometer: Nicolet Company of the United States; Varian Mercury 300 BB nuclear magnetic resonance instrument, VARIAN Company of the United States; Brucker AM 400 superconducting nuclear magnetic resonance instrument, Brucker Company of the United States; RE-52A rotary evaporator, Shanghai Yarong Biochemical Instrument Factory.

[0046] Reagent

[0047] Macroporous adsorption resin: LSA-10, produced by Xi'an Lanxiao Technology Co., Ltd.

[0048] Lymphocyte Separation Solution (Ficoll-Hypaque): Shanghai Huajing Bio-Technology Co...

Embodiment 3

[0125] Embodiment 3: proline-glycine cyclic tetrapeptide (C 14 h 20 N 4 o 4 ) Molecular biological detection of enhancing fish immune function

[0126] 1. Materials and methods

[0127] (1) Materials and reagents

[0128] carp( Cyprinus carpio ) with an average body weight of 65.3 ± 5.48 g and were obtained from the Fishery Farm of Shaanxi Fisheries Research Institute. Temporarily raise 15d, spare. The isolated compound I is the proline-glycine cyclic tetrapeptide (C 14 h 20 N 4 o 4 ), with a purity of 98.57%, provided by the Disease Laboratory of the Fisheries Department of the College of Animal Science and Technology, Northwest Agriculture and Forestry University; chloroform, isopropanol, and ethanol (produced by Tianjin Fuyu Fine Chemical Co., Ltd.; Produced by Shanghai Suolaibao Biotechnology Co., Ltd.; Reverse Transcription Kit (Fuzytes Company, USA); Real-time Fluorescence Quantitative PCR Reaction Kit (Dalian Bao Bioengineering Company).

[0129...

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Abstract

The invention relates to immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404). The compound is obtained by the fermentation and separation of microorganism Bacillussimplex DR-834. As shown by numerous tests, the compound can be used for preparing drugs for enhancing the immune function of animals such aquatic animal fish. A crude fermentation product of the Bacillussimplex DR-834 containing cyclic tetrapeptide can be used as feedstuff. By using the proline-glycocoll cyclic tetrapeptide, the mRNA (Messenger RNA) expression level of interleukin 1-beta gene and nitric oxide synthase in blood is obviously increased compared with contrast, and the immunity of aquatic animals can be obviously improved. The result of a virus attacking test indicates that the cyclic tetrapeptide is remarkable in immune protection. In test groups, the average death rate is 26.67%, and the rate of a contrast group is 73.33% and the relative immune protection rate of the contrast group is 63.63%.

Description

technical field [0001] The present invention relates to a new compound isolated from microbial metabolites——proline-glycine cyclic tetrapeptide (C 14 h 20 N 4 o 4 ), and the use of the compound. Background technique [0002] Since the middle of the 20th century, with the rapid development of the aquaculture industry, pharmaceutical feed additives such as antibiotics, olaquindox, and quinolones have been widely used worldwide. These additives have obvious effects in preventing diseases and promoting animal growth, and have played a role that cannot be ignored in a specific period. With the emergence of the theme of green, safety, health and environmental protection, which has attracted much attention from human beings, people have paid attention to food safety issues. While the drug feed additive has significant effects, it also has adverse effects such as residue, toxicity, and "three causes" (teratogenic, carcinogenic, and mutagenic), and the side effects after use are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A61K38/12C07K5/12A61P37/04A23K20/147A23K50/80
CPCY02A40/818
Inventor 石存斌吴淑勤王庆王高学任燕赖迎迢王芳
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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