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Pseudoalteromonas fish killing and application thereof in aquaculture

A pseudoalteromonas and aquaculture technology, applied in the field of microorganisms, can solve problems such as difficulty in antagonizing pathogenic bacteria and immune function and promoting intestinal function, difficult and efficient application of bacterial disease technology, and few application technology reports, etc. Intestinal function, length increase, the effect of length increase

Active Publication Date: 2021-08-10
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fermenting fish and shrimp feed with Lactobacillus can increase the utilization rate of feed, improve the intestinal flora structure of farmed animals, and reduce the colonization of pathogenic bacteria; There are many, and the existing strains generally have a single function, and it is difficult to achieve the synergistic effect of antagonizing pathogenic bacteria, immune function, and promoting intestinal function, making it difficult to efficiently apply the existing microbial prevention and control of bacterial diseases in aquaculture

Method used

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  • Pseudoalteromonas fish killing and application thereof in aquaculture
  • Pseudoalteromonas fish killing and application thereof in aquaculture
  • Pseudoalteromonas fish killing and application thereof in aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Effect of Pseudoalteromonas piscicida 2013082515 on shrimp culture

[0050] 1. Material method

[0051] 1.1 Preparation of Pseudoalteromonas piscicida 2013082515 preparation

[0052] Take the 2013082515 strain of Pseudoalteromonas piscicida preserved at -80°C, streak it on 2216E solid medium, culture it at 28°C for 24 hours to obtain a single colony, and inoculate a single colony into the seed culture solution of Pseudoalteromonas piscicida In 28°C shake flask culture for 24 hours to obtain the seed liquid, then inoculate the seed liquid in the fermentation medium of Pseudoalteromonas ichthicida for 24 hours to carry out aerobic fermentation, the temperature is controlled at 28°C; when the OD600 value of the fermentation liquid reaches From 1.5 to 1.7 hours, the fermentation was terminated, and the fermentation broth was obtained, and the strain concentration of the fermentation broth was determined by plate counting.

[0053] The 16S rDNA base sequence of Pseudoalter...

Embodiment 2

[0090] Effect of Pseudoalteromonas piscicida 2013082515 on the culture of flounder

[0091] 1. Material method

[0092] 1.1 Preparation of Pseudoalteromonas piscicida 2013082515 preparation

[0093] Take the 2013082515 strain of Pseudoalteromonas piscicida preserved at -80°C, streak it on 2216E solid medium, culture it at 28°C for 24 hours to obtain a single colony, and inoculate a single colony into the seed culture solution of Pseudoalteromonas piscicida In 28°C shake flask culture for 24 hours to obtain the seed liquid, then inoculate the seed liquid in the fermentation medium of Pseudoalteromonas ichthicida for 24 hours to carry out aerobic fermentation, the temperature is controlled at 28°C; when the OD600 value of the fermentation liquid reaches From 1.5 to 1.7 hours, the fermentation was terminated, and the fermentation broth was obtained, and the strain concentration of the fermentation broth was determined by plate counting.

[0094] 1.2 Experimental flounder

[00...

Embodiment 3

[0131] Effect of Pseudoalteromonas piscicida 2013082515 on the breeding of bay scallops

[0132] 1. Material method

[0133] 1.1 Preparation of Pseudoalteromonas piscicida 2013082515 preparation

[0134] Take the 2013082515 strain of Pseudoalteromonas piscicida preserved at -80°C, streak it on 2216E solid medium, culture it at 28°C for 24 hours to obtain a single colony, and inoculate a single colony into the seed culture solution of Pseudoalteromonas piscicida In 28°C shake flask culture for 24 hours to obtain the seed liquid, then inoculate the seed liquid in the fermentation medium of Pseudoalteromonas ichthicida for 24 hours to carry out aerobic fermentation, the temperature is controlled at 28°C; when the OD600 value of the fermentation liquid reaches From 1.5 to 1.7 hours, the fermentation was terminated, and the fermentation broth was obtained, and the strain concentration of the fermentation broth was determined by plate counting.

[0135] 1.2 Bay scallop larvae used...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to an application of pseudoalteromonas fish killing in fish and shrimp culture. The strain is preserved in the China Center for Type Culture Collection in Wuhan on November 12, 2020, and the preservation number is CCTCC NO: M 2020731. The bacterial strain has broad-spectrum anti-vibrio activity, has strong antagonistic activity on Aeromonas salmonicida, can enhance expression of fish lysozyme, tumor necrosis factors and cell interleukin genes after being orally taken, can significantly improve the immune level of aquaculture animal body fluid, and in addition, the strain can also significantly increase the length of intestinal microvilli of aquaculture animals, has a trend of increasing muscle thickness and villi length, is beneficial to improving the intestinal function of the aquaculture animals, realizes the synergistic effect of antagonizing pathogenic bacteria, immunizing and promoting the intestinal function, and more effectively prevents and controls bacterial diseases in aquaculture.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of a strain of Pseudoalteromonas piscicida in fish and shrimp cultivation Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily to be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] There are many kinds of microorganisms in the natural environment, and some microorganisms can interact with each other, including symbiosis, symbiosis, parasitism, antagonism and predation. In order to control the proliferation of pathogenic microorganisms in the environment and in vivo, the antagonistic characteristics between microorganisms can be used to achieve the purpose of controlling diseases. At present...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/741A61P31/04A61P37/04A23K50/80A23K10/18A01K61/13C12R1/01
CPCA61K35/741A61P31/04A61P37/04A23K50/80A23K10/18A01K61/13Y02A40/81
Inventor 王秀华王枫林杨冰张庆利
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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