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Production technology of fluorescent phycocyanin and crystal and product thereof

A phycocyanin and fluorescence technology, which is applied to the preparation method of peptides, algae/moss peptides, peptide sources, etc., can solve the problems of loss of protein function, inability to store for a long time, loss of biological activity, etc., and achieve high fluorescence activity and preservation conditions. require small effects

Active Publication Date: 2006-06-14
福建省神六保健食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the phycocyanin has certain health benefits, and the health food composition of the phycocyanin has certain applications in the field of fluorescence detection, since the protein is in a state of freeze-drying or precipitation, the functional groups of the phycocyanin are very Easy to inactivate, when affected by some physical factors such as heat, ultraviolet radiation, high pressure and surface tension, etc. or chemical factors, there will often be a loss of biological activity, exposure of some side chain groups, and changes in some physical and chemical properties And the change of biochemical properties leads to the loss of protein function, so it needs to be prepared and used immediately, and cannot be stored for a long time

Method used

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  • Production technology of fluorescent phycocyanin and crystal and product thereof
  • Production technology of fluorescent phycocyanin and crystal and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] 1. Preparation of fluorescent phycocyanin

[0127] cell disruption

[0128] Weigh the Spirulina platensis into a beaker, wash it twice with distilled water, add a phosphate buffer solution with a pH value of 6.0 and a concentration of 0.005 mol / L, and stir evenly. Ultrasonic crush at 1800W in an ice bath (0°C) for 30 minutes, place the crushed algae liquid in a pre-cooled high-speed refrigerated centrifuge, centrifuge at 8000 revolutions per minute (rpm) at 0°C for 60 minutes, remove the precipitate, and collect supernatant;

[0129] salting out

[0130] Add solid ammonium sulfate to the supernatant to make it reach 25% saturation, let it stand at 0°C for 10 hours, freeze and centrifuge at 6000 revolutions per minute (rpm) at 0°C for 60 minutes, and discard the precipitate. Continue to add solid ammonium sulfate to the supernatant to 80% saturation, let stand at low temperature (0°C) for 10 hours, and centrifuge at 6000 revolutions per minute (rpm) at 0°C for 60 minu...

Embodiment 2

[0150] 1. Preparation of fluorescent phycocyanin

[0151] cell disruption

[0152] Weigh Spirulina platensis into a beaker, wash it twice with distilled water, add a phosphate buffer solution with a pH value of 6.8 and a concentration of 0.005 mol / L, and stir evenly. Ultrasonic crush at 1800W in an ice bath (0°C) for 40 minutes, place the crushed algae liquid in a pre-cooled high-speed refrigerated centrifuge, centrifuge at 7000 revolutions per minute (rpm) at 2°C for 90 minutes, remove the precipitate, and collect Serum;

[0153] salting out

[0154] Add solid ammonium sulfate to the supernatant to make it reach 25% saturation, let it stand at 2°C for 10 hours, centrifuge at 7000 revolutions per minute (rpm) at 2°C for 90 minutes, and discard the precipitate. Continue to add solid ammonium sulfate to the supernatant to 80% saturation, let stand at low temperature (2°C) for 10 hours, and centrifuge at 7000 revolutions per minute (rpm) at 2°C for 90 minutes, discard the supe...

Embodiment 3

[0174] 1. Preparation of fluorescent phycocyanin

[0175] cell disruption

[0176] Weigh Spirulina platensis into a beaker, wash it twice with distilled water, add a phosphate buffer solution with a pH value of 7.1 and a concentration of 0.005 mol / L, and stir evenly. Ultrasonic crush at 1800W for 35 minutes in an ice bath (0°C), place the crushed algae liquid in a pre-cooled high-speed refrigerated centrifuge, and centrifuge at 9000 revolutions per minute (rpm) at 4°C for 50 minutes to remove the precipitate and collect Serum;

[0177] salting out

[0178] Add solid ammonium sulfate to the supernatant to make it reach 20% saturation, let stand at 5°C for 10 hours, centrifuge at 9000 revolutions per minute (rpm) at 4°C for 35 minutes, and discard the precipitate. Continue to add solid ammonium sulfate to the supernatant to 80% saturation, let stand at low temperature (5°C) for 10 hours, and centrifuge at 9000 revolutions per minute (rpm) at 4°C for 35 minutes, discard the su...

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Abstract

The present invention relates to a protein, crystal preparation process and its product, in particular, it relates to a fluorophycocyanin which is extracted from algae under the general condition and can emit strong fluorescence, and can convert it into crystal form. It can be extensively used in the fields of scientific research, medicine, detection and telecommunication, etc.

Description

technical field [0001] The present invention relates to a protein and crystal manufacturing process and products, in particular to phycocyanin extracted from algae under normal conditions and capable of emitting strong fluorescence and transforming it into a crystal form, which can be used in scientific research, medical treatment, detection, etc. , Telecom and other fields of application. Background technique [0002] As early as the beginning of the last century, it was reported abroad that there were strongly fluorescent red, violet and blue proteins in cyanobacteria and red algae. In 1910, Kylin named these pigment proteins as "Phycochromo-proteins" for the first time. This light-harvesting pigment protein that appears in large quantities in red algae, blue-green algae and cryptophyta is the phycobiliproteins (Phycobiliproteins, PBP) that is currently in urgent need of development, which mainly includes phycoerythrin (Phycoerythrin, PE), fluorescent algae Protein (Phyc...

Claims

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Application Information

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IPC IPC(8): C07K14/405C07K1/36
Inventor 骆建华刘维国刘俊
Owner 福建省神六保健食品有限公司
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