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Method for separating cell and special separating liquid for cell

A technology for separating cells and separating liquid, applied in the field of separating cells, can solve problems such as restricting large-scale cell separation, and achieve the effects of shortening the time for sample addition and cell separation, strong operability, and simple cell separation method.

Inactive Publication Date: 2006-06-28
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to ensure the integrity of the liquid surface, the person who adds the sample needs to be trained for a certain period of time and needs to operate patiently in order to obtain good results, which seriously restricts the large-scale cell separation.

Method used

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  • Method for separating cell and special separating liquid for cell
  • Method for separating cell and special separating liquid for cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the preparation of cell separation liquid

[0025] Add paraffin oil with a concentration of 50% by volume to Ficoll to obtain a cell separation liquid.

Embodiment 2

[0026] Embodiment 2, the preparation of cell separation liquid

[0027] First, the Percoll was made to reach the physiological osmotic pressure by a conventional method, and then triglyceride with a volume percentage concentration of 80% was added thereto to obtain a cell separation solution. If necessary, after centrifugation, other concentrations of Percoll working solution can be added layer by layer at normal speed.

Embodiment 3

[0028] Embodiment 3, the preparation of cell separation liquid

[0029] Phospholipids with a concentration of 500% by volume were added to Metrizamide to obtain a cell separation liquid.

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PUM

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Abstract

The invention discloses a cell separating method and special cell separating medium. The special cell separating medium gains is solvent gained by adding surface active agent into general cell separating medium. The cell separating method includes the following steps: mixing the surface active agent and current cell separating medium in given volumetric proportion; centrifuging to gain special cell separating medium; adding cell separating sample into the special cell separating medium; and centrifuging; colleting cloud layer cell and washing to gain purified cell. The invention greatly shortens sample adding and cell separating time, simplifies operation. And it is fit for large-scale separating. Its effect is stable and reliable. And high purity goal cell can be gained. The separating method is simple, and has strong operability. The invention has high practical application value and broad market prospect in cell biology, functional genome, and cell engineering fields.

Description

technical field [0001] The invention relates to a method for separating cells and a special cell separation liquid. Background technique [0002] The emergence and development of stem cells (totipotent stem cells, pluripotent stem cells, multipotent stem cells) has greatly promoted the progress of cell engineering technology. Modern biotechnologies such as cell therapy, cytological diagnosis of diseases, cell vaccines, cell chips, and RNAi have gradually been widely used at the cell, body, and group levels, and have become an important part of modern biotechnology. Therefore, stem cells play an important role and have great commercial value in both basic theoretical research and production promotion. [0003] Density gradient centrifugation separates cells of different sizes and qualities, enabling the separation and purification of different types of cells. After in vitro amplification and engineering operations, it can be used in the fields of cell transgenesis, disease d...

Claims

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Application Information

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IPC IPC(8): C12N5/07
Inventor 连正兴李海李宁
Owner CHINA AGRI UNIV
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