Method for cultivating cymbidium goeringii seed by tissue culture

A tissue culture and seed technology, applied in the field of plant tissue culture, can solve problems such as difficulty in finding wild Chunlan plants, destruction of natural resources, time-consuming and troublesome efficiency, etc., and achieve the effect of simple operation, reduced damage, and feasible methods

Inactive Publication Date: 2006-07-12
江苏春辉生态农林股份有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the failure to solve the problem of mass reproduction, farmers went to the mountains to dig a large number of wild Chunlan, and the natural resources were seriously damaged. It is difficult to find wild Chunlan plants in areas with relatively convenient transportation.
[0004] Since ancient times, the

Method used

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Experimental program
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Embodiment Construction

[0013] Below is the best mode that the present invention adopts Chunlan seed tissue culture seedling raising method:

[0014] a), explants: in October to November, uncracked Chunlan capsules from yellow to brown were taken as explants;

[0015] b), Seed treatment: Soak in 70-75% alcohol for 20-30 seconds or dip cotton pads in 70-75% alcohol to wipe the surface of the Chunlan capsule, especially pay attention to the vertical groove of the capsule, and bring it into the operating room for standby. In the clean workbench, cover it with sterile paper, cut the Chunlan capsule longitudinally with a dissecting scalpel, take out the Chunlan powdery seeds, put them into a sterilized fine cloth bag, soak and disinfect with 70-75% alcohol for 30-45 seconds , rinse 3-4 times with sterile water, drain the water, and then put it into a sterilized 0.08-0.12mol / L NaoH solution for 8-10 minutes, rinse 3-4 times with sterile water, drain the water, and finally put Put into 10.5±2% sodium hypoc...

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Abstract

The invention relates to a method of seedling with tissue culturing by using spring orchid seed, comprising the following steps: taking the capsule of not broke up from spring orchid as explant; immersing with alcohol or wiping it with absorbent cotton dipped with alcohol, cutting up the capsule to fetch out the pulverulent seed, loading it into sheeting bag, immersing it in the alcohol, putting it in the NaOH solution with concentration being 0.08-0.12mol/l for 8-10 minutes, putting it into sodium hypochlorite solution of 10.5í‚2% for 6-8 minutes; sowing the treated seeds on the culturing medium for seed germination, lighting 14-16 hours fir every day, with the luminous intensity being 1000-1500lux, temperature 23í‚2 Deg C, culturing for 160-180 days, and growing out original bulb; transferring the original bulb to the culturing medium for seed germination the same with mentioned above for successive transfer culture, for 50-60 days, and growing out new sprout or original bulb, cutting the original bulb continuously for successive transfer culture and reaching the geometric extension. The invention employs method of tissue culturing by using spring orchid seed, to breed species of high quality and to choose orchid plant from seed seedling.

Description

Technical field: [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for raising seedlings of Chunlan by adding different components to a basic medium. Background technique: [0002] Chunlan is a kind of ground-growing orchid. It is native to China and has a long history of cultivation. It not only has beautiful flowers and elegant fragrance, it is known as "a gentleman among flowers"; In addition, it also has high medicinal value and can treat various diseases. With the growth of the world economy, the demand for rare and precious varieties of orchids in China, especially Chunlan, is also increasing. [0003] The germination of Cymbidium plant seeds has accumulated a lot of materials, but the seeds of some ground orchids in the Cymbidium plants are not easy to germinate artificially, and such seeds are generally called difficult-to-germinate seeds. Although there are many varieties of Chunlan, they are all selected from th...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/00C12N5/04
Inventor 吴有光顾海燕曹亮
Owner 江苏春辉生态农林股份有限公司
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