Acetolysis analyzing method without acetic anhydride for spore powder in quaternary sediment

Abstract

The invention relates to acetolysis analysis method without acetic anhydride for spore and pollen in the Quaternary Period sediment. It includes the following steps: taking sample preprocessing: removing carbonate rock and silica by dilute hydrochloric acid and hydrofluoric acid, heating by thick hydrochloric acid, filtering; centrifugal dewatering; making solution of acetolysis analysis method without acetic anhydride from 75-85% glacial acetic acid, 15-25% vitriol; adding the solution into sample, whisking, heating, and the ratio is 1:2-3; adding water into the sample after cooling, centrifuging, pouring out the acid solution, adding water and taking centrifugation repeating for 2-3 times to remove acid liquid, adding little water into sample, washing in the ultrasonic wave cleaning machine, sieving, and taking centrifugation and storing. The optimized method the weight ratio of glacial acetic acid and vitriol is 80%: 20%. It is convenience to operate in lab.

Description

Technical field: [0001] The invention relates to an experimental technique for paleontological fossil spores and pollen, in particular to an improved vinegar hydrolysis analysis method for spores and pollen in Quaternary sediments. technical background: [0002] Pollen analysis is an applied work for researchers to study the plant spores and pollen preserved in the stratum. It is of great significance to the study of vegetation evolution and climate change in the geological historical period. At the same time, it is used in geological exploration, search for coal, petroleum, energy and other mineral deposits. Plays an important role. As a prerequisite for pollen research, how to use chemical and physical methods to extract fossil pollen from rocks and make them into slices that can be preserved forever, which is commonly referred to as pollen analysis. This is a specialized technique and an important part of palynology. Similarly, fossil pollen is also an important content of Qua...

AI Technical Summary

Problems solved by technology

In the preparation, the requirements for safe operation are extremely high, and no open flames should appear, so the operation is very dangerous and difficult to master, so it is not easy to promote

Secondly, acetic anhydride is a flammable product designated by the state and is subject to national control. Usually, a letter of introduction from the unit is required and approval from the Public Security Bureau is required. Therefore, the purchase of laboratory procedures is extremely cumbersome, which seriously affects the time and efficiency of experimental analysis.