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Method for inducing and cultivating hairy root system of Saussurea medusa

A cultivation method and hairy root technology, applied in the field of plant biology, can solve the problems of low plant biomass, low plant diversity index, and difficulty in restoring ecosystems, and achieve high frequency, reliable material source and material basis, and operation cycle. short effect

Inactive Publication Date: 2006-09-06
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plant community structure in the area where snow lotus is distributed is simple, and the diversity index of plants is very low. At the same time, due to the low biomass of plants, once the ecosystem is damaged, it will be difficult to restore

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for inducing and cultivating the hairy roots of Saussurea jellyfish, comprising the steps of:

[0036] (1) Sterilize the seeds of Saussurea jellyfish, inoculate them into hormone-free MS medium on an ultra-clean workbench, and cultivate them at 20°C to obtain sterile seedlings;

[0037] (2) Get the aseptic seedlings of Saussurea jellyfish, cut the aseptic seedlings, cotyledons, petioles and hypocotyls into small sections of about 1 cm, and transfer them to the pre-medium for 2 days in dark at 20°C; the pre-medium includes the following Content components: 2.0mg / L MS medium, 2.0mg / L 2,4-D auxin, 0.2mg / L cytokinin,

[0038] (3) Immerse the various explants of the pre-cultivated 2D Saussurea jellyfish liquid in the Agrobacterium rhizogenes solution, soak for 8min, transform the pre-cultivated explants through the mediation of the Agrobacterium rhizogenes A4 strain, and then in the absence of Absorb the excess bacterial liquid on the bacterial filter paper;

[00...

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Abstract

The invention relates to a method for inducing and cultivating the trichomes root system of jellyfish saussurea involucrate. The method comprises the following steps: first inoculating sterile seed into MS culture medium without hormone to get sterile seedling; then taking the cotyledon of seedling as explant, proceeding conversion through agricillin; finally proceeding bacterial inhibition and inductive culture for conversed explant and enlarged culture for trichomes root. The invention can provide the inducing frequency of jellyfish saussurea involucrate trichomes root, and the culture period is short by taking seedling cotyledon as explant.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for efficiently inducing and cultivating hairy roots of Saussurea jellyfish. Background technique [0002] Jellyfish Saussurea jellyfish is the authentic Tibetan medicine "Qiagao Suba". It is mainly distributed in the alpine periglacial zone at an altitude of 3900-5600 meters. It is a unique plant species on the Qinghai-Tibet Plateau. It is a rare medicinal plant. It is used in Tibetan medicine to treat rheumatoid arthritis, gynecological diseases and altitude sickness. Due to the extremely harsh natural conditions in the distribution area of ​​snow lotus: the temperature is low, the cold season is dry and long, the warm season is short, and the plant growth season is only 90-150 days. Jellyfish snowdrops usually take 3-5 years to mature and bloom, and the general height is about 30 cm, or even lower. Make the natural resources of jellyfish snowdrop extre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/00A01H4/00C12N5/04A01G1/00A01G7/00
Inventor 王莉李毅
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST