Dopamine detection method based on surface enhanced resonance Raman spectrum

Abstract

The invention relates to an analytically chemical detection method of dopamine and particularly relates to a dopamine detection method based on a surface enhanced resonance Raman spectrum. The dopamine detection method comprises the following steps: taking gold nano-particles obtained by taking sodium citrate as a reducing agent to reduce chloroauric acid as an SERRS substrate; taking ferric iron as a central body to be simultaneously coordinated with citrate ions remained on the surfaces of the gold nano-particles and dopamine molecules in a solution to be detected to form a citric acid-iron-DA complex, so as to selectively capture DA molecules in the solution to be detected; and forming resonance by the citric acid-iron-DA complex and Raman exciting light to generate a very strong SEERS signal so as to obtain an SEERS characteristic fingerprint signal of the DA molecules and realize high-sensitivity SERRS detection on the DA molecules. The detection method provided by the invention has the advantages that the needed materials are simple, the operation period is short and the cost is low; and the detection lower limit is very low.

Description

technical field [0001] The invention relates to an analytical chemical detection method for dopamine, in particular to a dopamine detection method based on surface-enhanced resonance Raman spectroscopy (SERRS). Background technique [0002] Since Martin Fleischmann and his collaborators discovered the surface-enhanced Raman scattering (SERS) phenomenon in 1974, and Jeanmaire and Van Duyne two scientists gave a theoretical explanation of this amazing discovery in 1977, SERS technology has been developed for nearly 40 years. SERS technology, because it provides rich molecular fingerprint information, can distinguish the structural differences of isomers, can distinguish the different orientations of the same molecule to be tested on the SERS substrate, and can capture the molecule to be tested to achieve selective detection, so It is widely studied and applied, and it is an advanced analysis and detection method. The study also found that: when the wavelength of the excitatio...

AI Technical Summary

Problems solved by technology

Traditional DA analysis and detection methods include: high performance liquid chromatography (HPLC), capillary electrophoresis (CE), etc. The detection sensitivity and selectivity of these methods are very high, but the sample preparation process is complicated, and some even require well-trained Professional technicians perform complex pretreatment on samples, such as separation, purification and other professional operations, so simple and rapid detection of DA cannot be achieved.

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