Cyanogen-less hemolysin for blood cell analyzer and prepn. method
A blood cell analyzer and hemolysin technology are applied in the field of cyanogen-free hemolysin and its preparation, which can solve the problems of complex formulation and high cost of hemolysin, and achieve the effects of low use cost, easy measurement and simple formulation.
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[0037] Example 1
[0038] The non-cyanide hemolysin of the present invention is prepared according to the following ratio:
[0039] (1) Cetyltrimethylammonium chloride 5g (mass percentage is 0.5%)
[0040] (2) 4-Phenylimidazole 1g (0.1% by mass)
[0041] (3) Sodium chloride 5g (mass concentration is 5g / L)
[0042] (4) Three distilled water 1L
[0043] Raw materials: cetyltrimethylammonium chloride, Fluka, 250g, 52366; 4-phenylimidazole, Fluka, 100g, 78726; sodium chloride, Beijing Chemical Reagent Company, 500g, batch number 050411.
[0044] Preparation method: Mix cetyltrimethylammonium chloride, 4-phenylimidazole, and sodium chloride in the above proportions, add water to 1L, shake at room temperature until it is uniform, and then put it in the filter. A 0.22 micron cellulose acetate filter membrane soaked in distilled water is used to filter the above solution three times.
Example Embodiment
[0045] Example 2
[0046] The non-cyanide hemolysin of the present invention is prepared according to the following ratio:
[0047] (1) Dodecyl trimethyl ammonium chloride 30g (mass percentage is 3%)
[0048] (2) 1,2,4-triazole 10g (mass percentage is 1%)
[0049] (3) Sodium chloride 10g (mass concentration is 10g / L)
[0050] (4) Three distilled water 1L
[0051] Raw materials: dodecyltrimethylammonium chloride, Fluka, 250g, 17104; 1,2,4-triazole, Fluka, 50g, 90630; sodium chloride, Beijing Chemical Reagent Company, 500g, batch number 050411.
[0052] Preparation method: Mix dodecyltrimethylammonium chloride, 1,2,4-triazole, and sodium chloride in the above proportions, add water to 1L, shake at room temperature until it is uniform, and then place in the filter A 0.22 micron cellulose acetate filter membrane soaked in three-distilled water was used to filter the above solution three times.
Example Embodiment
[0053] Example 3
[0054] The non-cyanide hemolysin of the present invention is prepared according to the following ratio:
[0055] (1) Dodecyl trimethyl ammonium bromide 50g (5% by mass)
[0056] (2) 4-Phenylimidazole 15g (mass percentage is 1.5%)
[0057] (3) Sodium chloride 20g (mass concentration is 20g / L)
[0058] (4) Three distilled water 1L
[0059] Raw materials: dodecyltrimethylammonium bromide, Fluka, 250g, 44240; 4-phenylimidazole, Fluka, 100g, 78726; sodium chloride, Beijing Chemical Reagent Company, 500g, batch number 050411.
[0060] Preparation method: Dodecyltrimethylammonium bromide, 4-phenylimidazole, and sodium chloride are mixed according to the above ratio, add water to 1L, shake at room temperature until it is even, then put it in the filter A 0.22 micron cellulose acetate filter membrane soaked in water filters the above solution three times.
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