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Cyanogen-less hemolysin for blood cell analyzer and prepn. method

A blood cell analyzer and hemolysin technology are applied in the field of cyanogen-free hemolysin and its preparation, which can solve the problems of complex formulation and high cost of hemolysin, and achieve the effects of low use cost, easy measurement and simple formulation.

Inactive Publication Date: 2006-09-20
北京索通医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the past, cyanide was used to measure hemoglobin, but it was rarely used due to the high toxicity of cyanide. Currently, cyanide-free hemolysin is mostly used for hemoglobin determination and white blood cell differential count, but the formula of these cyanide-free hemolysin is more complicated ,higher cost

Method used

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  • Cyanogen-less hemolysin for blood cell analyzer and prepn. method
  • Cyanogen-less hemolysin for blood cell analyzer and prepn. method
  • Cyanogen-less hemolysin for blood cell analyzer and prepn. method

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Embodiment 1

[0038] The non-cyanide hemolysin of the present invention is prepared according to the following ratio:

[0039] (1) Cetyltrimethylammonium chloride 5g (mass percentage is 0.5%)

[0040] (2) 4-Phenylimidazole 1g (0.1% by mass)

[0041] (3) Sodium chloride 5g (mass concentration is 5g / L)

[0042] (4) Three distilled water 1L

[0043] Raw materials: cetyltrimethylammonium chloride, Fluka, 250g, 52366; 4-phenylimidazole, Fluka, 100g, 78726; sodium chloride, Beijing Chemical Reagent Company, 500g, batch number 050411.

[0044] Preparation method: Mix cetyltrimethylammonium chloride, 4-phenylimidazole, and sodium chloride in the above proportions, add water to 1L, shake at room temperature until it is uniform, and then put it in the filter. A 0.22 micron cellulose acetate filter membrane soaked in distilled water is used to filter the above solution three times.

Embodiment 2

[0046] The non-cyanide hemolysin of the present invention is prepared according to the following ratio:

[0047] (1) Dodecyl trimethyl ammonium chloride 30g (mass percentage is 3%)

[0048] (2) 1,2,4-triazole 10g (mass percentage is 1%)

[0049] (3) Sodium chloride 10g (mass concentration is 10g / L)

[0050] (4) Three distilled water 1L

[0051] Raw materials: dodecyltrimethylammonium chloride, Fluka, 250g, 17104; 1,2,4-triazole, Fluka, 50g, 90630; sodium chloride, Beijing Chemical Reagent Company, 500g, batch number 050411.

[0052] Preparation method: Mix dodecyltrimethylammonium chloride, 1,2,4-triazole, and sodium chloride in the above proportions, add water to 1L, shake at room temperature until it is uniform, and then place in the filter A 0.22 micron cellulose acetate filter membrane soaked in three-distilled water was used to filter the above solution three times.

Embodiment 3

[0054] The non-cyanide hemolysin of the present invention is prepared according to the following ratio:

[0055] (1) Dodecyl trimethyl ammonium bromide 50g (5% by mass)

[0056] (2) 4-Phenylimidazole 15g (mass percentage is 1.5%)

[0057] (3) Sodium chloride 20g (mass concentration is 20g / L)

[0058] (4) Three distilled water 1L

[0059] Raw materials: dodecyltrimethylammonium bromide, Fluka, 250g, 44240; 4-phenylimidazole, Fluka, 100g, 78726; sodium chloride, Beijing Chemical Reagent Company, 500g, batch number 050411.

[0060] Preparation method: Dodecyltrimethylammonium bromide, 4-phenylimidazole, and sodium chloride are mixed according to the above ratio, add water to 1L, shake at room temperature until it is even, then put it in the filter A 0.22 micron cellulose acetate filter membrane soaked in water filters the above solution three times.

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Abstract

The invention relates to a no-cyanogen hemolysin and the manufacture method used for blood corpuscle analyzer that includes quaternary ammonium salt, imidazole or one material from imidazole ramification, alkali metal chlorinated salt and water. It could accurately test the thickness of hemoglobin and has no toxicity. It is low cost and has high reliability.

Description

Technical field [0001] The invention belongs to the technical field of reagents for medical detection, and specifically relates to a cyanide-free hemolysin used in a blood cell analyzer and a preparation method thereof. Background technique [0002] The determination of white blood cell count and hemoglobin concentration has become an important indicator for the analysis of patient blood samples during medical diagnosis. Many diseases usually cause abnormal changes in the number of white blood cells or hemoglobin concentration. If abnormal changes in white blood cells or hemoglobin can be detected in time, it will help patients Timely diagnosis and treatment. [0003] There are five types of white blood cells in normal blood circulation, including lymphocytes, monocytes, neutrophils, basophils, and eosinophils. The latter three are collectively called granulocytes. Therefore, people divide white blood cells into There are three subgroups of lymphocytes, monocytes and granulocytes...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N33/72C07C211/63C07D235/00C07D249/08
Inventor 蔡佩娟卫五保
Owner 北京索通医疗技术有限公司
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