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Polypeptide growth factor copolymer and its preparation method and use

A peptide growth factor and copolymer technology, applied in the field of long half-life polypeptide growth factor copolymer and its preparation, can solve the problem of short half-life of cytokines and the like

Inactive Publication Date: 2006-09-27
李晓光
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The complex condenses on the surface of the cell membrane, and then vesicles into the cell and decomposes due to the presence of lysosomes, resulting in a very short half-life of cytokines in vivo

Method used

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  • Polypeptide growth factor copolymer and its preparation method and use
  • Polypeptide growth factor copolymer and its preparation method and use
  • Polypeptide growth factor copolymer and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: long half-life nerve growth factor copolymer formed by combining nerve growth factor and chitosan

[0050] Nerve growth factor (NGF) is one of the most important biologically active substances in the nervous system, and it is also the earliest discovered and most typical neurotrophic factor. It affects the survival and differentiation of certain neurons in the peripheral and central nervous systems. Experiments in recent years have demonstrated that cells in the central nervous system (cerebral cortex and spinal cord) of adults or animals can regenerate after injury. The copolymer with neurotrophic activity of the present invention is applied to the local active matrix of central nervous system injury to play the role of regenerative scaffold and promote regeneration. For example, traumatic brain injury and spinal cord injury.

[0051] The production method of long half-life nerve growth factor copolymer, comprises the following steps:

[0052] 1. Add 1...

Embodiment 2

[0059] Example 2: Long half-life basic fibroblast growth factor copolymer with "arms" formed by combining basic fibroblast growth factor with chitosan

[0060] Basic fibroblast growth factor (bFGF) can promote wound healing and is used for burn wounds, chronic wounds, body surface ulcers, bedsores, and fresh wounds (including trauma, donor site wounds, surgical wounds, etc.). And it has been used as a genetic engineering drug in clinical application.

[0061] The production method of the long half-life basic fibroblast growth factor copolymer with "arm", comprising the following steps:

[0062] 1. Under the condition of pH8.5, add 10ml of 5% cyanogen bromide solution to 100ml 20% chitosan solution, and react for 10 minutes to activate the hydroxyl group of chitosan;

[0063] 2. Under the condition of pH 8.5, add 3ml of 10% methylene diamine to the above solution to make it undergo dehydration reaction, and methylene diamine is the "arm" to be connected;

[0064] 3. Add 500ug...

Embodiment 3

[0067] Embodiment 3: the long half-life epidermal growth factor copolymer that epidermal growth factor is combined with chitosan

[0068] Epidermal growth factor (EGF) has a wide range of biological effects, and it has a strong mitogenic effect on epithelial cells from a variety of tissue sources. It is suggested that it may act as a wound healing factor. In addition, EGF also stimulates the proliferation of various mesenchymal cells, including corneal endothelial cells, vascular endothelial cells, vascular smooth muscle cells, fibroblasts and chondrocytes, etc., which in turn provides the necessary nutrients for the growth of epithelial cells, including the entire epidermis. material basis. For example, experiments by Brown et al. demonstrated its role in the healing of cut and burn wounds.

[0069] The production method of long-half-life epidermal growth factor copolymer comprises the following steps:

[0070] 1. Add 1ml of 15% glutaraldehyde to 100ml of 30% chitosan solu...

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Abstract

The invention discloses a polypeptide growth factor copolymer and preparing method and application, which is characterized by the following: the polypeptide growth factor connects with biological medical polymers carrier substance through conjugated linkage or hydrocarbon chain to form copolymer; the invention has fouw methods to prepare it. The invention can be used for preparing the drug of rebuilding animal body lesion and coloboma organization or organ.

Description

technical field [0001] The invention relates to a growth factor copolymer in the field of bioengineering and its preparation method and application, in particular to a long half-life polypeptide growth factor copolymer and its preparation method and application. Background technique [0002] Tissue engineering is the science of applying the principles and methods of engineering and life sciences to form and regenerate living tissues to reconstruct human diseased and defective tissues or organs. Tissue engineering relies on biomedical materials to provide a framework for tissue regeneration, and biomedical materials are given vitality through the application of tissue engineering. The combination of the two provides the possibility to realize the construction of living human "parts", and then design and construct complete human organs. [0003] Polypeptide growth factors are a series of biologically active polypeptides that play an important role in the process of embryogene...

Claims

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Application Information

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IPC IPC(8): C07K17/08A61K38/03
Inventor 李晓光
Owner 李晓光
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