Quick Feulgen dyeing method

A dyeing method and fast technology, which is applied in the field of biological cell and tissue staining, can solve the problems of unstable dyeing effect, application limitations, high accuracy requirements, etc., and achieve the effect of stable dyeing effect and simple operation
CN1837772AInactive Publication Date: 2006-09-27JINAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JINAN UNIVERSITY
Publication Date
2006-09-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention relates to a quick Feulgen dyeing method, wherein, putting all of the cell smear, organization press, frozen section, drip section or paraffin section into 4~6N hydrochloric acid for 3~10min hydrolysis; then, putting into Schiff's reagent for 8~15min; flushing with tap water for 2~5min, rinsing with distilled water for 2~3 times, taking fast gradient ethanol dehydration, and packing. This invention is fast, simple and stable, and cuts dye time greatly with well effect.
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Description

technical field

[0001] The invention belongs to the technical field of biological cell and tissue staining, in particular to a fast Feulgen staining method. Background technique

[0002] During the pathological diagnosis of routine cell smears, tissue prints, frozen sections, rapid paraffin sections or drop slides, when it is difficult to judge the benign and malignant states of pathological cells and tissues alone, the measurement of nuclear DNA content and Ploidy analysis can provide important reference. Therefore, it is required that the Feulgen staining method of nuclear DNA can meet the requirements of clinical rapid pathological diagnosis.

[0003] According to the principle of nuclear DNA cytochemical staining, the two steps of hydrochloric acid hydrolysis and Schiff's reagent staining in the staining process are very critical and important. The nuclear DNA is hydrolyzed with hydrochloric acid to break the glycosidic bond between the deoxyp...

Claims

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