Quick Feulgen dyeing method

A dyeing method and fast technology, which is applied in the field of biological cell and tissue staining, can solve the problems of unstable dyeing effect, application limitations, high accuracy requirements, etc., and achieve the effect of stable dyeing effect and simple operation

Inactive Publication Date: 2006-09-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the accuracy of the output power of the microwave oven is very high during the dyeing process, the dyeing effect is unstable, and the Feulgen staining solution can only be used once
Therefore, its application in clinical diagnosis is greatly limited

Method used

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Examples

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Effect test

Embodiment 1

[0014] From 10 Kunming mice 30 days after birth, smears of liver cell suspension were prepared, dried at room temperature, and fixed with 4% paraformaldehyde for 3 minutes. The hepatocyte smear of each mouse was stained by Feulgen according to the following method: 5N hydrochloric acid and Schiff's reagent were placed in a 60°C incubator and preheated to 60°C, and the fixed hepatocyte smear was hydrolyzed in 5N hydrochloric acid for 5 minutes , and then placed in Schiff's reagent for staining for 10 minutes, rinsed with tap water for 3 minutes, rinsed with distilled water for 3 times, dehydrated with rapid gradient ethanol, and mounted. Both 5N hydrochloric acid and Schiff’s reagent must be preheated to 60°C, and the entire hydrochloric acid hydrolysis process and Schiff’s reagent dyeing process are carried out at a temperature of 60°C.

[0015] Adjust the LEICA LB microscope light source according to Kohler lighting requirements, insert a 535 / 35 band-pass filter between the o...

Embodiment 2

[0018] The rapid Feulgen staining method of the present invention comprises the following steps: put 4N hydrochloric acid and Schiff's reagent into a 50°C incubator and preheat to 50°C, put the liver tissue print in 4N hydrochloric acid for 3 minutes, and then put it into Schiff's reagent for staining 8 minutes, rinsed with tap water for 2 minutes, rinsed twice with distilled water, dehydrated with rapid gradient ethanol, and sealed. The entire hydrochloric acid hydrolysis process and Schiff's reagent staining process were carried out at a temperature of 50°C. 4N hydrochloric acid solution and Schiff's reagent can be used repeatedly without any treatment.

Embodiment 3

[0020] The rapid Feulgen staining method of the present invention comprises the following steps: put 6N hydrochloric acid and Schiff's reagent into a thermostat at 70°C and preheat to 70°C, put the frozen slices of liver tissue into 6N hydrochloric acid for hydrolysis for 10 minutes, and then put them into Schiff's reagent for staining 15 minutes, rinsed with tap water for 5 minutes, rinsed with distilled water for 3 times, dehydrated with rapid gradient ethanol, and sealed. The entire hydrochloric acid hydrolysis process and Schiff's reagent staining process were carried out at a temperature of 70°C. 6N hydrochloric acid solution and Schiff's reagent can be used repeatedly without any treatment.

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Abstract

This invention relates to a quick Feulgen dyeing method, wherein, putting all of the cell smear, organization press, frozen section, drip section or paraffin section into 4~6N hydrochloric acid for 3~10min hydrolysis; then, putting into Schiff's reagent for 8~15min; flushing with tap water for 2~5min, rinsing with distilled water for 2~3 times, taking fast gradient ethanol dehydration, and packing. This invention is fast, simple and stable, and cuts dye time greatly with well effect.

Description

technical field [0001] The invention belongs to the technical field of biological cell and tissue staining, in particular to a fast Feulgen staining method. Background technique [0002] During the pathological diagnosis of routine cell smears, tissue prints, frozen sections, rapid paraffin sections or drop slides, when it is difficult to judge the benign and malignant states of pathological cells and tissues alone, the measurement of nuclear DNA content and Ploidy analysis can provide important reference. Therefore, it is required that the Feulgen staining method of nuclear DNA can meet the requirements of clinical rapid pathological diagnosis. [0003] According to the principle of nuclear DNA cytochemical staining, the two steps of hydrochloric acid hydrolysis and Schiff's reagent staining in the staining process are very critical and important. The nuclear DNA is hydrolyzed with hydrochloric acid to break the glycosidic bond between the deoxyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 夏潮涌金日男
Owner JINAN UNIVERSITY
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