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Quality detecting method for Radix-Acanthopanacis-leaf total flavone extract frozen dry powder injecta

A quality inspection method, the technology of Acanthopanax acanthopanax leaves, applied in the direction of freeze-drying transportation, powder transportation, measuring devices, etc., can solve the problems of large deviation of measurement results, lack of measurement methods for the content of monomeric flavonoids, etc., to achieve stable quality, The effect of high-efficiency methods

Inactive Publication Date: 2006-10-25
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The quality standard of Ciwujia Injection, which uses Acanthopanax rhizome as medicine, uses rutin as a reference substance, and uses spectrophotometry to measure the total flavonoid content, but rutin is not the flavonoid compound with the highest content in Ciwujia, so the determination The results have large deviations; and there is a lack of content determination methods for monomeric flavonoids; and so far, there is no preparation of total flavonoids in Acanthopanax leaves and corresponding quality detection methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: a kind of component content detection method of the total flavonoids extract of Acanthopanax senticosus leaves for injection freeze-dried powder injection, comprising the following steps and conditions:

[0021] 1. Observation and TLC identification of lyophilized powder of total flavonoids extract from Acanthopanax senticosus for injection

[0022] (1) Take the lyophilized powder of the total flavonoids extract of Acanthopanax senticosus leaves for injection and observe, the content is yellow-brown loose solid;

[0023] (2) Freeze-dried powder of total flavonoids extract from Acanthopanax senticosus for injection, 10 mg of the content for injection, put it in a conical flask, add 5 ml of 95% ethanol, ultrasonically treat it to dissolve, and use it as the test solution. Another reference substance of hyperin is taken, and 95% ethanol is added to make a solution containing 0.50 mg per 1 ml, as the reference substance solution. Then take 1 g of Acanthopanax...

Embodiment 2

[0035] 1. Observation and TLC identification of the freeze-dried powder of the total flavonoids extract of Acanthopanax senticosus for injection

[0036] (1) Take the lyophilized powder of the total flavonoids extract of Acanthopanax senticosus leaves for injection and observe that the content is a yellow-brown loose solid; the smell is slightly bitter, and the taste is slightly bitter;

[0037] (2) 20 mg of the content of the freeze-dried powder injection of the total flavonoid extract of Acanthopanax senticosus leaves for injection was placed in an Erlenmeyer flask, and 5 ml of 60%% ethanol was added, and dissolved by ultrasonic treatment, as the test solution. Take another hyperin reference substance, add 60% ethanol to make a solution containing 0.25mg per 1ml, as the reference substance solution. Take Acanthopanax senticosus reference medicinal material powder 1g again, prepare contrast medicinal material solution with the preparation method of embodiment 1 and absorb eac...

Embodiment 3

[0049] 1. Observation and TLC identification of the freeze-dried powder of the total flavonoids extract of Acanthopanax senticosus for injection

[0050] (1) Take the lyophilized powder of the total flavonoids extract of Acanthopanax senticosus leaves for injection and observe that the content is a yellow-brown loose solid; the smell is slightly bitter, and the taste is slightly bitter;

[0051] (2) 30mg of lyophilized powder content of Acanthopanax senticosus leaves total flavonoids extract for injection was placed in a conical flask, 5ml of 70% ethanol was added, and dissolved by ultrasonic treatment, as the test solution. Take another hyperin reference substance, add 70% ethanol to make a solution containing 0.30mg per 1ml, as the reference substance solution. Prepare the control medicinal material solution by the preparation method of Example 1, absorb 3 μl of each of the above three solutions, and spot them on the same polyamide film respectively, and use benzene-methanol...

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Abstract

The present invention relates to a quality detection method of acanthopanax leaf total flavone extract freeze-dried powder injection. Said method includes the following several steps: using thin-layer chromatography to make identification, total flavone content and hyperin content determination and total flavone and monomer flavone content detection. Said method can ensure stable quality of said acanthopanax leaf total flavone extract freeze-dried powder injection.

Description

technical field [0001] The invention belongs to a quality detection method for freeze-dried powder injection of total flavonoids from Acanthopanax senticosus leaves for injection. Background technique [0002] The quality standard of Acanthopanax senticosus injection using the rhizome of Acanthopanax senticosus uses rutin as a reference substance, and the total flavonoid content is determined by spectrophotometry, and rutin is not the flavonoid with the highest content in Acanthopanax senticosus, so determine There is a large deviation in the results; and the method for measuring the content of monomeric flavonoids is lacking; and so far, the total flavonoids in Acanthopanax senticosus leaves have not been used as preparations and the corresponding quality testing methods have not been provided. SUMMARY OF THE INVENTION [0003] The invention provides a quality detection method for qualitative and quantitative detection of the total flavonoids extract of Acanthopanax senti...

Claims

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Application Information

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IPC IPC(8): A61K36/254A61K9/19G01N21/25G01N30/02G01N30/90G01N33/15
Inventor 宋凤瑞刘志强刘淑莹
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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