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Process for preparing high-purity amino acid and immune bioactive peptide

A technology of immune active peptides and amino acids, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, and unknown raw materials, etc., can solve the problems of low conversion rate of proteolytic protein, low utilization rate of animal blood, and low product concentration, etc., to achieve The effect of high product concentration, low production cost and high conversion rate

Inactive Publication Date: 2006-12-13
佟振宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for preparing high-concentration amino acids and immunoactive peptides to solve the problems of low utilization rate of animal blood, low conversion rate of protein hydrolyzed by proteolysis, low product concentration and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of crude enzyme solution: Take 1Kg of fresh porcine pancreas, remove fat tissue manually, then chop it up with a sterilized knife, place it in a clean and dry container, add 0.5L of distilled water, and use Homogenize after colloid mill grinding, then add a small amount of NaOH to the homogenate, adjust the pH value to 7.5, then store the homogenate at room temperature for 8 hours, then filter the homogenate with a clean silk cloth, and take the filtrate .

[0022] 2. Take 1 portion of fresh pig blood, put it in a dry container, add 0.2 times the volume of distilled water, stir until uniform, then add NaOH, adjust the pH to 7.9, heat the liquid to 100 degrees, and keep boiling for 20 minutes. Then lower the temperature to 25 degrees, add 0.1 times the volume of the crude enzyme solution obtained in step 1 to the liquid, mix well, then put it into a constant temperature box, adjust the temperature at 39 degrees, and place it for 20 hours. Then take it out...

Embodiment 2

[0024] 1. Preparation of crude enzyme solution: Take 1Kg of fresh porcine pancreas, remove fat tissue manually, then chop it with a sterilized knife, place it in a clean and dry container, add 0.8L of distilled water, and use Homogenize after colloid mill grinding, then add a small amount of NaOH to the homogenate, adjust the pH value to 7.5, then store the homogenate at room temperature for 8 hours, then filter the homogenate with a clean silk cloth, and take the filtrate .

[0025] 2. Take 1 portion of fresh pig blood, put it in a dry container, add 0.5 times the volume of distilled water, stir until uniform, then add NaOH, adjust the pH to 7.5, heat the liquid to 100 degrees, and keep boiling for 25 minutes. Then cool down to 30 degrees, add 0.3 times the volume of crude enzyme solution obtained in step 1 to the liquid, mix evenly, then put it into a constant temperature box, adjust the temperature at 40 degrees, and place it for 25 hours. Then take it out, heat and boil f...

Embodiment 3

[0027] 1. Preparation of crude enzyme solution: Take 1Kg of fresh porcine pancreas, remove fat tissue by hand, then chop it up with a sterilized knife, place it in a clean and dry container, add 1L of distilled water, and use colloid After grinding and homogenizing, add a small amount of NaOH to the homogenate to adjust the pH value to 7.5, then store the homogenate at room temperature for 8 hours, then filter the homogenate with a clean silk cloth, and take the filtrate.

[0028] 2. Take 1 part of fresh pig blood, put it in a dry container, add 0.8 times the volume of distilled water, stir until uniform, then add NaOH, adjust the pH to 8.2, heat the liquid to 100 degrees, and keep boiling for 30 minutes. Then cool down to 45 degrees, add 0.5 times the volume of crude enzyme solution obtained in step 1 to the liquid, mix well, then put it into a constant temperature box, adjust the temperature at 45 degrees, and place it for 35 hours. Then take it out, heat and boil for 10 min...

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PUM

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Abstract

The invention relates to a method for preparing high concentrate amino acid and immunological active peptide, which belongs to the biotechnological domain. The invention includes the preparation of rough enzymatic fluid: getting fresh pig pancreas, adding water, grinding, equalizing, adjusting pH value,filtering and adopting the filtering medium; getting fresh pork blood, adding water, adjusting pH value to 7.9-8.2, heating the liquid to 100 degrees, then cooling to 25-45 degrees, adding rough enzymatic fluid into the liquid, mixing evenly, then placing then into the constant temperature cabinet, adding right amount of acidum hydrochloricum, adjusting pH value to 3.5-5.5, filtering the liquid to obtain the filter liquor. The invention has many dominances including high conversion rate of protein enzyme-hydrolysis albumen and product concentration and low cost of manufacture making use of purified amino acid and immunological reinforced peptide of this invention, and has good immunity.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] Animal blood, mainly pig and cow blood, is the most unsatisfactory and very large amount of by-products in the slaughtering industry. Except for a part of pig blood that can be used for food, most of the rest cannot be used for valuable purposes. Deep Processing. Researchers at home and abroad have always attached great importance to the comprehensive utilization of animal blood, but there has been no significant progress. [0003] In terms of the utilization of small biological molecules, people mainly focus on the extraction of amino acids. There are usually two ways to prepare amino acids from blood protein: enzymatic hydrolysis and acid hydrolysis. In 1968, Mullinger et al. used papain to hydrolyze pig blood, and the amino acid yield was less than 10%, and the amino acid concentration was only 1mg / ml. In 1994, Zheng Yuanqi and others used the ammonium sulfate met...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12P21/00A61K35/14A61K35/39
Inventor 佟振宇
Owner 佟振宇
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