Macro creatine kinase agarose electrophoresis kit and preparation method thereof

Abstract

The invention discloses a huge creatine-kinase agarose electrophoresis agent box and making method in the medical biological domain, which comprises the following parts: agarose gel plate, Tris buffer, soluble bartitone-barbitone electrophoresis buffer, substrate, substrate buffer and color-developing agent. The invention can test serum sample to avoid false positive occurrence of CK-M B due to immune inhibiting method, which provides correct testing report for clinical.

Description

technical field [0001] The invention belongs to the field of medical biology and relates to a kit and a preparation method thereof. More particularly, the invention relates to a giant creatine kinase agarose electrophoresis kit which can be used for clinical disease detection and a preparation method thereof. Background technique [0002] Creatine kinase (CK) is a dimer composed of M and B subunits, and there are three isozymes in the cytoplasm: CK-MM, CK-MB and CK-BB. There is another isoenzyme in the mitochondria of cells, called CK-Mt (also known as giant CK). Giant CK is divided into giant CK1 and giant CK2. Giant CK1 is a complex of CK isoenzymes and autoantibodies. Because of its large molecular weight, it is not easily excreted in the body, and because of its long half-life, it persists in the blood for a long time. The literature believes that CK1 has no obvious connection with diseases, and some giant CK1 is even detected in healthy human blood. Giant CK2 is an o...

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Problems solved by technology

[0007] However, the above-mentioned full-automatic or semi-automatic electrophoresis instruments are expensive and cannot be purchased by general hospitals. In addition, the special matching kits cannot be used for testing small batches of specimens. The cost will be greatly increased, and it is often necessary to identify a suspicious specimen in clinical practice, so it is not suitable for identification.

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