Fusarium solani mutant for producing chitosan enzyme and its uses in preparing chitosan
A technology for producing Fusarium solani and chitosanase, which is applied in fungi, fermentation and other directions, can solve the problems of small enzyme production and low enzyme activity, and achieves short enzymatic hydrolysis time, high content and high effective ingredients. Effect
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Embodiment 1
[0033] Example 1: Screening of Fusarium solani mut.hydrochitosans Fs107 CGMCC No.1720.
[0034]Samples were taken from soybean farmland soil in Dezhou, Liaocheng, Heze, Zaozhuang and other places, and eight strains with high chitosanase production were obtained through screening by the flat transparent circle method, followed by UV mutagenesis and flat plate transparent circle method. Mutant strain of Fusarium solani with high chitosanase production. Subsequently, the shake flask fermentation culture and the small fermenter culture were carried out, and the fermentation was carried out under the conditions that the culture temperature was 25° C., the pH of the fermentation medium was 5.6, the rotation speed was 150 rpm, and the ventilation rate was 2.0 vvm. Among them, the screened Fusarium solani mut.hydrochitosans Fs107 CGMCC No.1720 of the present invention has good enzyme production activity, and the enzyme activity reaches 240mU / ml.
[0035] The above-mentioned plate tra...
Embodiment 2
[0039] Example 2: Morphological characteristics of Fusarium solani mut.hydrochitosans Fs107 CGMCC No.1720 strain.
[0040] The characteristics of the morphological observation are: the growth diameter of the bacteria on the PDA plate for 4 days is about 4.2cm, the aerial hyphae are fluffy, brownish white, the opposite side of the colony is light brown or light red, and the conidia have two types of large and small. Large conidia are colorless, sickle-shaped, with 3-4 septa, 30-50μm×4.6μm in size; small conidia are oblong or cylindrical, clustered, colorless, with 1-2 septa, rarely Or not produced; chlamydospore spherical or oval, smooth or rough surface, terminal or interstitial, solitary or twin, size 8~10μm×7.0~9.5μm.
[0041] According to the morphological observation, the chitosanase-producing bacterial strain of the present invention screened is determined to be a bacterial strain of the genus Fusarium.
[0042] As a result of experimental comparison, its enzyme-producin...
Embodiment 3
[0043] Example 3: Preparation of oligochitosan using Fusarium solani mut.hydrochitosans Fs107 CGMCC No.1720. The fermentation temperature was 20°C
[0044] (1) Strain selection: Fusarium solani mut.hydrochitosans Fs107CGMCC No.1720;
[0045] (2) Slant culture: inoculate the strains on potato medium, add agar with a mass volume ratio of 1.6%, and culture statically for 72 hours at 20°C;
[0046] (3) Seed cultivation: the bacterial strain cultivated in step (2) is connected to 1~2 loops with inoculation loop under aseptic conditions in 200ml of glucose containing mass volume ratio 2%, 0.1% peptone, 0.1% yeast powder, pH value In the basic inorganic salt medium of 5.6, shake culture for 48 hours at 20°C to obtain the seed solution;
[0047] (4) Expansion cultivation: with the inoculation amount of 5% volume ratio, the seed solution is inoculated in 400ml containing the glucose that mass volume ratio is 2%, 0.1% peptone, 0.1% yeast powder, 0.1% casein, pH value is 5.6 In the ba...
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