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Process for biologically synthesizing aspartame

A biosynthesis and aspartame technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unstable process parameters, high production costs, low yields, etc., and reduce separation and purification. Cost, high selectivity, effect of reducing reaction steps

Inactive Publication Date: 2007-01-24
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Some are more complicated, and the production cost is higher, and some aspartame laboratory results are OK, but the process parameters are unstable after the expansion of production, and the yield is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Strain fermentation medium (weight percentage) is bran, corn flour, starch, peptone or bean cake powder: 5-40%, sucrose, glucose, maltose or potato juice 2-30%, KH 2 PO 4 0.05%~1.00%, MgSO 4 0.01%~0.7%, FeCl 3 0.0005%~0.005%, yeast extract (powder) 0.01%~1.5%, (NH 4 ) 2 SO 4 0.01% to 1.2%. The conditions for the fermentation of Absidia spp bacterial strains in the culture medium are: temperature 25~45°C, pressure 0.03~0.12Mpa, air volume 1:0.1~1:1, pH5.5~9.5, rotating speed 250~550r.p.m , and the culture time is 2 to 4 days. Set the volume ratio of the enzyme liquid obtained from the above fermentation to aspartic acid, phenylalanine, and methanol as 1:1, and synthesize aspartame under the conditions of 20-65°C and pH value of 2.5-9.5 Sweet, the enzymatic reaction time is 2 to 24 hours, and the weight of each substance of aspartic acid, phenylalanine, and methanol is calculated according to the molecular weight.

Embodiment 2

[0024] Strain fermentation medium (weight percentage) is bran, corn flour, starch, peptone or bean cake powder: 5-40%, sucrose, glucose, maltose or potato juice 2-30%, KH 2PO 4 0.05%~1.00%, MgSO 4 0.01%~0.7%, FeCl 3 0.0005%~0.005%, yeast extract (powder) 0.01%~1.5%, (NH 4 ) 2 SO 4 0.01% to 1.2%. The conditions for the fermentation of Schizophyllumcommune Fr. strains in the culture medium are: temperature 25-45°C, pressure 0.03-0.12Mpa, air volume 1:0.1-1:1, pH 5.5-9.5, speed 250-550r. p.m, culture time 2-4 days. Set the volume ratio of the enzyme liquid obtained from the above fermentation to aspartic acid, phenylalanine, and methanol as 1:1, and synthesize aspartame under the conditions of 20-65°C and pH value of 2.5-9.5 Sweet, the enzymatic reaction time is 2 to 24 hours, and the weight of each substance of aspartic acid, phenylalanine, and methanol is calculated according to the molecular weight.

Embodiment 3

[0026] Strain fermentation medium (weight percentage) is bran, corn flour, starch, peptone or bean cake powder: 5-40%, sucrose, glucose, maltose or potato juice 2-30%, KH 2 PO 4 0.05%~1.00%, MgSO 4 0.01%~0.7%, FeCl 3 0.0005%~0.005%, yeast extract (powder) 0.01%~1.5%, (NH 4 ) 2 SO 4 0.01% to 1.2%. The conditions for the fermentation of Pseudomonas aeruginosa (P.aeruginosa) strains in the culture medium are: temperature 25~45°C, pressure 0.03~0.12Mpa, air volume 1:0.1~1:1, pH 5.5~9.5, rotating speed 250~ 550r.p.m, culture time 2-4 days. Set the volume ratio of the enzyme liquid obtained from the above fermentation to aspartic acid, phenylalanine, and methanol as 1:1, and synthesize aspartame under the conditions of 20-65°C and pH value of 2.5-9.5 Sweet, the enzymatic reaction time is 2 to 24 hours, and the weight of each substance of aspartic acid, phenylalanine, and methanol is calculated according to the molecular weight.

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Abstract

The present invention is one-step enzyme process of synthesizing aspartame, and the one-step enzyme process is developed on the basis of available biological synthesizing process. The one-step enzyme process of synthesizing aspartame includes fermentation with some strain to obtain enzyme and the subsequent direct synthesis of aspartame with aspartic acid, phenylalanine and methanol as substrate. The present invention has reduced reaction steps, simplified production process and lowered production cost.

Description

technical field [0001] The invention relates to a method for synthesizing sweeteners, in particular to a new method for the biosynthesis of aspartame. Background technique [0002] Aspartame is a new type of high-efficiency sweetener, its sweetness is about 200 times that of sucrose, and its calories are only 1 / 200 of sucrose. Regular food does not produce dental caries, does not affect blood sugar, does not cause obesity, high blood pressure, and coronary heart disease. The United Nations Food and Agriculture Organization (FAO) and the World Health Organization (WHO) evaluated it as safe and reliable, and it was identified as an international A (I) sweetener by the United Nations Joint Committee on Food Additives (JECFA). At present, the sweetener has been approved for use in more than 130 countries and regions, and is widely used in more than 9,000 kinds of beverage, food and pharmaceutical products. So far, no natural aspartame has been found in nature, and its producti...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12R1/65C12R1/85C12R1/385C12R1/125
Inventor 梁智群吴满刚陈桂光张云开黄时海李楠陈山岭
Owner GUANGXI UNIV
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