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Primary hypertension relative gene and its detecting method and usage

A kind of essential hypertension, gene technology, applied in the direction of genetic engineering, plant genetic improvement, botanical equipment and methods, etc.

Inactive Publication Date: 2012-07-25
FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the α1A receptor gene variation, the relationship between the α1A receptor gene variation and essential hypertension, and the detection methods, kits and Its application prospects in forecasting and new drug development have no patents or reports at home and abroad

Method used

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  • Primary hypertension relative gene and its detecting method and usage
  • Primary hypertension relative gene and its detecting method and usage
  • Primary hypertension relative gene and its detecting method and usage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Rapid detection of C2547 / G polymorphism by direct sequencing

[0064] 1. Obtaining of C2547 / G polymorphic amplification fragment

[0065] The C2547 / G polymorphic amplified fragment (618bp) was obtained by polymerase chain reaction (PCR). For each PCR plate, add 2 samples of DNA template to be tested, 2 cases of control reaction, and 2 cases of blank control reaction without DNA sample. See Table 2 and Table 3 for C2547 / G polymorphic amplification primers and amplification conditions.

[0066] Table 2 C2547 / G polymorphism detection primers

[0067]

[0068] Table 3 C2547 / G polymorphic PCR amplification system and amplification conditions

[0069]

[0070] 2. Identification of C2547 / G polymorphic genotype by direct sequencing of dideoxynucleotide terminal termination method

[0071] After the DNA fragment containing the C2547 / G polymorphism is successfully amplified, first use a commercial PCR product purification plate (for example: Millipore MultiScr...

Embodiment 2

[0086] Example 2 Scientific evidence of linkage between human chromosomal region 8p22 where ADRA1A is located and human essential hypertension and blood pressure level

[0087] Methods: 148 Han Chinese hypertensive families were selected, and the method in Example 1 was used to conduct linkage analysis with SAGE / SIBPAL2 software. The results are shown in Table 5.

[0088] Table 5 The linkage between 8p22 and human blood pressure levels in 148 Han Chinese hypertensive families

[0089]

[0090] Conclusion: As shown in Table 5, there is a linkage between the human chromosome region 8p22 where ADRA1A is located and human essential hypertension and blood pressure levels.

Embodiment 3

[0091] Frequency distribution of embodiment 3C2547 / G polymorphism in case-control samples

[0092] 1. For the description of the clinical characteristics of the case-control samples, see Table 6.

[0093] Table 6 Description of clinical characteristics of case-control samples

[0094]

[0095] Values ​​are mean ± standard deviation. NS, not statistically significant; BMI, body mass index.

[0096] 2. Frequency distribution of C2547 / G polymorphism in case-control samples

[0097] Using the method in Example 1, the above-mentioned cases (n=503) and controls (n=504) were sequenced and analyzed respectively, and the results were statistically analyzed. The results are shown in Table 7.

[0098] Table 7 Frequency distribution of C2547 / G polymorphism in case-control samples

[0099]

[0100] Results: As shown in Table 7, the distribution of the C2547 / G polymorphic G allele in the cases was significantly higher, indicating that the ADRA1A gene is a human essential hypertens...

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Abstract

The present invention relates to a variant of the alpha- 1A adrenergic receptor gene. Compared with the registered sequence of the alpha-IA adrenergic receptor gene in the GenBank, namely UniGene : HS 520951, db xref : Locus ID:148; db xref. MIM:104221, this variant is different in the G allele for the C2547 / G polymorphic variation. The allele of the C2547 / G variation is ''G'' in the 266 position of the sequence set forth. by SEQ ID NO 1. The present invention further relates to the in vitro method to detect the essential hypertension associated gene, and to detect the risk for essential hypertension, and the corresponding kits for detecting the alpha-lA adrenergic receptor gene. The present invention also relates to the usage of said gene and the kits in the prevention, diagnosis and treatment of essential hypertension.

Description

technical field [0001] The invention relates to a disease-related gene, in particular to an essential hypertension-related gene. The present invention also relates to a mutant alpha-1 adrenoceptor gene and its detection method and application. The invention also relates to a method for predicting the relative risk of essential hypertension in vitro and a kit for detecting alpha-1A adrenoceptor gene. Finally, the present invention relates to the application of the above-mentioned mutated gene, detection method, and method for predicting the relative risk of essential hypertension in vitro in the prevention, diagnosis or treatment of essential hypertension. Background technique [0002] Hypertension is a common and frequently-occurring disease that endangers human health, and the coronary heart disease and stroke caused by it have become the primary cause of human death due to disease. There is an urgent need for effective methods and drugs that can prevent, diagnose or tre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/12
CPCC12Q2600/156C12Q1/6883
Inventor 顾东风葛东亮李宏帆
Owner FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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