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Compound for poisoning pine wood nematode and application thereof

A technology of pine wood nematodes and compounds, applied in the field of compounds for killing pine wood nematodes, achieving the effects of low product toxicity, low product cost, and good poisonous killing effect

Inactive Publication Date: 2007-02-07
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the honokiol compound isolated from M.tripetala and its toxic and killing activity against pine wood nematode (B.xylophilus).

Method used

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  • Compound for poisoning pine wood nematode and application thereof
  • Compound for poisoning pine wood nematode and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] 1, the separation and preparation of compound honokiol (honokiol)

[0011] (1) Collect three-petal magnolia leaves from the botanical garden of Kunming Institute of Botany, Chinese Academy of Sciences, wash them with tap water, dry them in a 50°C oven, grind them into powder, extract them with 70% ethanol for 72 hours, and extract them three times. The extracts are combined, filtered and concentrated Dissolved in water, then extracted with ethyl acetate, the extract was concentrated and then chromatographically separated on a silica gel column, and the sample was dry-loaded with a gradient of petroleum ether / acetone (9:1, 8:2, 7:3, 2:1) Elution;

[0012] (2) The eluate is detected by biological activity tracking to obtain active components, and after the active components are concentrated, they are chromatographically separated again on a silica gel column, loaded in a dry method, and eluted with petroleum ether / acetone (9:1). The liquid was concentrated and subjected ...

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Abstract

This invention involves a compound for killing Bursaphelenchus xylophilus and its application, belonging to biological pesticide technology. The compound is prepared from Magnolia tripetala leaves. The compound is honokiol with molecular formula of C18H18O2, and has the advantages of low cost and low toxicity and good effects for killing Bursaphelenchus xylophilus. The compound can be used to prepare a pesticide for Bursaphelenchus xylophilus.

Description

Technical field: [0001] The invention relates to a compound for poisoning and killing pine xylophilus and its application, belonging to the technical field of biological pesticides. Background technique: [0002] Pine wood nematode (Bursaphelenchus xylophilus) is a harmful organism that is extremely harmful to coniferous plants such as pine, and is a quarantine pest that is prohibited from entering my country. Pine xylophilus disease caused by pine wood nematode is one of the most dangerous forest diseases in the world. At present, the control methods of pine wood nematode mainly include chemical control, disease-resistant breeding, control of vector insect beetle, and treatment of diseased and dead wood, etc., but the effects are not obvious or have limitations. Therefore, the research and development of high-efficiency and low-toxicity pine wood nematodes has received widespread attention. [0003] Three-petal magnolia (Magnolia tripetala L.) belongs to Magnoliaceae (Mag...

Claims

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Application Information

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IPC IPC(8): C07C39/19A01N31/08A01P5/00
Inventor 张克勤洪林军李国红
Owner YUNNAN UNIV
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