Analysis element used for inspection method of specimen
An inspection method and component technology, applied in the field of analytical components, can solve the problems of deviation of measured values, failure of analyzers to achieve measurement accuracy, and failure to successfully meet the needs of simplicity.
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Embodiment 1
[0251] The resin tube portion of a 10 mL vacuum blood collection tube (which has an inner diameter of 13.5 mm, manufactured by TERUMO Corporation) was cut with a knife in such a manner that the shape of the rubber portion into which the puncture needle will be inserted remains unchanged. section. Then, the puncture needle is inserted into the rubber part of the cut blood collection tube, allowing air to pass in and out. In this state, the plunger portion of the syringe manufactured by TERUMO was inserted into the blood collection tube and moved to a position approximately 10 mm away from the rubber portion. Then, remove the needle. In this state, the piston part is pulled by a specified distance, thereby decompressing the tube. Next, the piston part is fixed in the tube (1) so that it cannot move. Then, whole blood collected in advance by using lithium heparin as an anticoagulant was injected into another syringe manufactured by TERUMO Corporation; furthermore, a puncture n...
Embodiment 2
[0254] A polystyrene (PS) resin material dry-type analysis element 20 for multi-component measurement as shown in FIG. 7 was prepared, having a width of about 24 mm and a length of about 28 mm. In the channel 23 (the width is 2 mm, the length is 10 mm, and the depth is 2 mm) in the lower part 22 of the dry analysis element 20 for multi-component measurement, there is provided: glass fiber filter paper (GF / D, produced by Whatman International Co., Ltd. produced by the company) 27 for retaining red blood cells and extracting plasma; The installation portion for installing the color reaction reagent 24 has a width of 5 mm, a length of 5 mm, and a depth of 2 mm. Each of FUJI DRI-CHEM test piece GLU-P (measurement wavelength: 505 nm, measurement component: glucose) or FUJI DRI-CHEM test piece TBIL-P (manufactured by Fujifilm Corporation) as the color reaction reagent 24 was Cut into pieces with a width of 2 mm and a length of 4 mm. Also, these sheets were placed on the mounting p...
Embodiment 3
[0262] [measured with MDF]
[0263] The relationship between the optical density and the amount of reflected light received was obtained by using light monochromated to 625 nm. The amount of received light can be measured using an 8-bit black and white CCD with excellent precision, and the range of the amount of received light is adjusted to be within the range of the calibration curve. Therefore, the optical density is obtained by the following method:
[0264] (1) The amount of light emitted from the light source is adjusted so that the amount of light received by the standard density plate is about 200 by using a standard density plate with an optical density of substantially 0 and inserting an attenuation filter. Then, by using 6 standard density plates, the relationship between the optical density and the amount of reflected light received was obtained. A calibration curve is thus prepared. When using a perforated stainless steel plate as an attenuation filter, the amo...
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Abstract
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