Method for obtaining chicken EPGCs unicellular clone

Abstract

The invention relates to chicken EPGCs single cell cloning method. Its feature is that using the fourth generation chicken EPGCs to prepare single cell cloning; culturing and passage to gain heredity uniform chicken EPGCs cell. The invention has simple and practical method, no need special apparatus demand, uses regular cell culture to finish single cell cloning, processes morphology and staining identification for the gained cell to improve that it has its primary characteristic. The produced cell can be directly used in developmental biology study, genome imprinting study fields etc. Because of the cell has little heredity changeability, it can be used to study its different affecting factors.

Description

technical field

[0001] The invention discloses a method for obtaining chicken EPGCs single-cell clone, belonging to the field of tissue and cell engineering. technical background

[0002] Embryonic primordial germ cells (EPGCs) are the precursor cells of gonocytes and the progenitor cells of sperm or eggs. In the field of pluripotent stem cell research, EPGCs have become a new source of stem cells and another hot spot in stem cell research in recent years. The vigorous development of single cell cloning technology is after the 1980s. On the one hand, it is due to the needs of theoretical research, and on the other hand, it is to adapt to the development of cell engineering research. The cell type in the cell line is heterogeneous, and the culture can be transformed from heterogeneous to homogeneous by single cell cloning, reducing the genetic variability of the culture. Cells obtained by single-cell cloning can be used in the research of cell characteristics and functions, ...

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