Use of protein finger print method in detecting kidney cancer cell protein finger print in blood

Abstract

This invention relates to a protein fingerprinting using stranded chromatography catch and analysis proteins. The method identifies and detects proteins in mass spectrometry. Analysis of the data is obtained in the form of computer-readable bar code format (protein fingerprint). The invention method can be used to detect blood cells in patients with renal cell carcinoma and normal cell protein fingerprints. The method is accurate, fast and convenient.

Description

technical field [0001] The invention relates to a novel method for analyzing proteins in biological samples, in particular to a protein fingerprint method for analyzing biological samples. The present invention also relates to protein fingerprinting in which the proteome is captured by retention chromatography and analyzed using a computer readable barcode format (protein fingerprint). Background technique [0002] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, identifying differences in proteins expressed within cells can be used in the diagnosis of disease and ultimately in drug development and disease treatment. However, differential analysis of protein expression and function requires the ability to resolve complex mixtures of intracellular molecules. However, many substances in cells often exist in trace amounts. The current methods for analyzing proteins have limitations ...

AI Technical Summary

Problems solved by technology

First, the method provides only two characteristics of biomolecules—mass and isoelectric point (pI)

Second, the resolution in each dimension is limited by the resolving power of the gel

For example, it is often difficult to distinguish between biomolecules that differ by less than 5% in mass or that differ in pI

Third, gels have limited capacity and sensitivity and may not be able to detect small amounts of expressed biomolecules

Fourth, small proteins or peptides with a molecular weight below about 10-20 kDa cannot be observed

Since human cell proteins are much more complex than potatoes, electrophoresis alone to identify protein fingerprints of diseases is far from meeting clinical needs