Use of protein finger print method in detecting kidney cancer cell protein finger print in blood

A protein and fingerprint technology, applied in the field of protein fingerprinting and protein fingerprinting for biological sample analysis, can solve the problems of limited gel capacity and sensitivity, complexity, and inability to observe molecular weight

Inactive Publication Date: 2007-03-28
北京赛尔迪生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the method provides only two characteristics of biomolecules—mass and isoelectric point (pI)
Second, the resolution in each dimension is limited by the resolving power of the gel
For example, it is often difficult to distinguish between biomolecules that differ by less than 5% in mass or that differ in pI
Third, gels have limited capacity a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Distinguishing Protein Fingerprints of Normal Human Cells and Kidney Cancer Patient Cells in Blood

[0064] (1) Experimental method

[0065] 1. Materials

[0066] 1. Source of specimens: 34 patients with renal cell carcinoma (20 males and 14 females, aged 32-77 years) from the hospital, without any treatment before operation, all were pathologically confirmed as renal cell carcinoma after operation. Among them, there were 26 cases of clear cell carcinoma, 3 cases of granular cell carcinoma, and 5 cases of mixed cell carcinoma. Pathological grade: 7 cases of grade I, 22 cases of grade II, 5 cases of grade III; clinical stage: 6 cases of stage I, 21 cases of stage II, 6 cases of stage III, and 2 cases of stage IV. Gender- and age-matched control serum came from 32 healthy volunteers and physical examination subjects.

[0067] 2. Reagents: Cibacron Blue, urea, acetonitrile, trifluoroacetic acid, and SPA (Sinapinic acid) were all purchased from Sigma.

[0068]...

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PUM

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Abstract

This invention relates to a protein fingerprinting using stranded chromatography catch and analysis proteins. The method identifies and detects proteins in mass spectrometry. Analysis of the data is obtained in the form of computer-readable bar code format (protein fingerprint). The invention method can be used to detect blood cells in patients with renal cell carcinoma and normal cell protein fingerprints. The method is accurate, fast and convenient.

Description

technical field [0001] The invention relates to a novel method for analyzing proteins in biological samples, in particular to a protein fingerprint method for analyzing biological samples. The present invention also relates to protein fingerprinting in which the proteome is captured by retention chromatography and analyzed using a computer readable barcode format (protein fingerprint). Background technique [0002] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, identifying differences in proteins expressed within cells can be used in the diagnosis of disease and ultimately in drug development and disease treatment. However, differential analysis of protein expression and function requires the ability to resolve complex mixtures of intracellular molecules. However, many substances in cells often exist in trace amounts. The current methods for analyzing proteins have limitations ...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N33/48
Inventor 王彭许洋
Owner 北京赛尔迪生物医药科技有限公司
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