Method for improving ox and sheep freezing sperm fertility rate and its dilute liquid used thereof

A technology for freezing semen and fertilization rate, which is applied in the field of diluent to improve the fertilization rate of frozen semen of cattle and sheep, can solve the problems of frozen semen vitality and low fertilization rate, and achieves the improvement of the acrosome integrity rate, the reduction of adverse effects, and the reduction of nutritional needs. Effect

Inactive Publication Date: 2007-04-11
NORTHWEST A & F UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for improving the fertilization rate of frozen semen of cattle and sheep and the dilu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: In the above step (1), dilute isothermally with physiological saline at a ratio of 1:3 at 30°C, centrifuge at 1000 rpm for 5 minutes, remove the seminal plasma once, and then adjust to the first dilution with physiological saline amount, then centrifuged at 1000 rpm for 5 minutes to remove the seminal plasma for the second time.

[0020] The diluent formula used for cryopreservation of semen is: sodium citrate.2H 2 O: 1.8g; Glucose: 3.2g; Fructose: 3.3g; Glycerin: 7ml; Egg Yolk: 20ml; Double distilled water 100ml; Tris 0.01g; Penicillin 100,000 units;

Embodiment 2

[0021] Embodiment 2: Dilute isothermally at 30°C with physiological saline in a ratio of 1:3.5, centrifuge at 1000 rpm for 5 minutes, remove the seminal plasma, then adjust to the amount diluted for the first time with physiological saline, and then dilute it at 1000 rpm Centrifuge for 5 minutes to remove seminal plasma.

[0022] The diluent formula used for cryopreservation of semen is: sodium citrate.2H 2 O: 2.2g; Glucose: 2.8g; Fructose: 3.1g; Glycerin: 7ml; Egg Yolk: 20ml; Double distilled water 100ml; Tris 0.01g; Penicillin 100,000 units;

Embodiment 3

[0023] Embodiment 3: Dilute isothermally at 30°C with physiological saline in a ratio of 1:4, centrifuge at 1000 rpm for 5 minutes, remove the seminal plasma, then adjust to the first dilution amount with physiological saline, and then dilute it at 1000 rpm Centrifuge for 5 minutes to remove seminal plasma.

[0024] The diluent formula used for cryopreservation of semen is: sodium citrate.2H 2 O: 2.0g; Glucose: 3.0g; Fructose: 3.48g; Glycerin: 5ml; Egg Yolk: 20ml; Double distilled water 100ml; Tris 0.01g; Penicillin 100,000 units;

[0025] Among the above-mentioned embodiments, embodiment 3 is the best embodiment.

[0026] In the operation of step (1), attention should be paid to: 1. The centrifugation conditions should be appropriate (including speed, time, and frequency). During centrifugation, the temperature of the semen should be ensured, and the operation should be fast and stable, so as to reduce the mechanical damage to the sperm. The above-mentioned centrifugation a...

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PUM

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Abstract

The invention belongs to technological field of animal reproductive physiology and fertility, more particularly relating to method raising fertilization rate of frozen semen from cow and sheep and dilution used in said method, overcoming present technique problems on frozen semen vitality and low rate of fertilization. Its technical arrangements are, adopting steps for elimination of seminal plasma in preservation of frozen semen, and said steps are twice dilution centrifuging of medium speed & short-term. Inclusions of necessary dilution are, sodium citrate 2H2O:1.8- 2.2g;glucose: 2.8-3.2g; glycerine: 5-7.0ml;yolk: 18- 24ml;double distilled water 100ml;Tris0.01g;penicillin 100 (unit); streptomycin 100 (unit) pH value within 6.8-7.2.

Description

Technical field: [0001] The method belongs to the field of animal reproduction physiology and reproduction technology, and specifically relates to a method for improving the fertilization rate of frozen semen of cattle and sheep and a diluent used therefor. Background technique: [0002] At present, the cryopreservation technology of semen is a relatively mature technology, which has been widely applied to various livestock and human beings, but there are still many defects and deficiencies in this technology, which restrict the further development of work such as livestock and poultry improvement . Some studies in recent years have shown that mammalian seminal plasma contains many harmful substances, which have adverse effects on semen cryopreservation. Therefore, how to take effective measures to remove its harmful components and retain or increase beneficial components is of great significance for promoting sperm acrosome reaction, sperm capacitation and improving fertil...

Claims

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Application Information

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IPC IPC(8): A61D19/00A61K45/00
Inventor 曹斌云姬云涛马毅祝发明
Owner NORTHWEST A & F UNIV
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