Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving ox and sheep freezing sperm fertility rate and its dilute liquid used thereof

A technology for freezing semen and fertilization rate, which is applied in the field of diluent to improve the fertilization rate of frozen semen of cattle and sheep, can solve the problems of frozen semen vitality and low fertilization rate, and achieves the improvement of the acrosome integrity rate, the reduction of adverse effects, and the reduction of nutritional needs. Effect

Inactive Publication Date: 2007-04-11
NORTHWEST A & F UNIV
View PDF0 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for improving the fertilization rate of frozen semen of cattle and sheep and the diluent used therein, so as to overcome the problems of low vitality and low fertilization rate of frozen semen in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: In the above step (1), dilute isothermally with physiological saline at a ratio of 1:3 at 30°C, centrifuge at 1000 rpm for 5 minutes, remove the seminal plasma once, and then adjust to the first dilution with physiological saline amount, then centrifuged at 1000 rpm for 5 minutes to remove the seminal plasma for the second time.

[0020] The diluent formula used for cryopreservation of semen is: sodium citrate.2H 2 O: 1.8g; Glucose: 3.2g; Fructose: 3.3g; Glycerin: 7ml; Egg Yolk: 20ml; Double distilled water 100ml; Tris 0.01g; Penicillin 100,000 units;

Embodiment 2

[0021] Embodiment 2: Dilute isothermally at 30°C with physiological saline in a ratio of 1:3.5, centrifuge at 1000 rpm for 5 minutes, remove the seminal plasma, then adjust to the amount diluted for the first time with physiological saline, and then dilute it at 1000 rpm Centrifuge for 5 minutes to remove seminal plasma.

[0022] The diluent formula used for cryopreservation of semen is: sodium citrate.2H 2 O: 2.2g; Glucose: 2.8g; Fructose: 3.1g; Glycerin: 7ml; Egg Yolk: 20ml; Double distilled water 100ml; Tris 0.01g; Penicillin 100,000 units;

Embodiment 3

[0023] Embodiment 3: Dilute isothermally at 30°C with physiological saline in a ratio of 1:4, centrifuge at 1000 rpm for 5 minutes, remove the seminal plasma, then adjust to the first dilution amount with physiological saline, and then dilute it at 1000 rpm Centrifuge for 5 minutes to remove seminal plasma.

[0024] The diluent formula used for cryopreservation of semen is: sodium citrate.2H 2 O: 2.0g; Glucose: 3.0g; Fructose: 3.48g; Glycerin: 5ml; Egg Yolk: 20ml; Double distilled water 100ml; Tris 0.01g; Penicillin 100,000 units;

[0025] Among the above-mentioned embodiments, embodiment 3 is the best embodiment.

[0026] In the operation of step (1), attention should be paid to: 1. The centrifugation conditions should be appropriate (including speed, time, and frequency). During centrifugation, the temperature of the semen should be ensured, and the operation should be fast and stable, so as to reduce the mechanical damage to the sperm. The above-mentioned centrifugation a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to technological field of animal reproductive physiology and fertility, more particularly relating to method raising fertilization rate of frozen semen from cow and sheep and dilution used in said method, overcoming present technique problems on frozen semen vitality and low rate of fertilization. Its technical arrangements are, adopting steps for elimination of seminal plasma in preservation of frozen semen, and said steps are twice dilution centrifuging of medium speed & short-term. Inclusions of necessary dilution are, sodium citrate 2H2O:1.8- 2.2g;glucose: 2.8-3.2g; glycerine: 5-7.0ml;yolk: 18- 24ml;double distilled water 100ml;Tris0.01g;penicillin 100 (unit); streptomycin 100 (unit) pH value within 6.8-7.2.

Description

Technical field: [0001] The method belongs to the field of animal reproduction physiology and reproduction technology, and specifically relates to a method for improving the fertilization rate of frozen semen of cattle and sheep and a diluent used therefor. Background technique: [0002] At present, the cryopreservation technology of semen is a relatively mature technology, which has been widely applied to various livestock and human beings, but there are still many defects and deficiencies in this technology, which restrict the further development of work such as livestock and poultry improvement . Some studies in recent years have shown that mammalian seminal plasma contains many harmful substances, which have adverse effects on semen cryopreservation. Therefore, how to take effective measures to remove its harmful components and retain or increase beneficial components is of great significance for promoting sperm acrosome reaction, sperm capacitation and improving fertil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61D19/00A61K45/00
Inventor 曹斌云姬云涛马毅祝发明
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com