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Method for cultivating and preparing active biochemical bacteria

The technology of active biochemical bacteria and culture medium is applied in the field of cultivation and preparation of high-efficiency active biochemical bacteria, and can solve the problems of increasing the crude protein and amino acid content of straws and the like.

Inactive Publication Date: 2007-04-11
南阳兴农生物技术开发有限公司
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AI Technical Summary

Problems solved by technology

[0003] In view of the above situation, the purpose of the present invention is to provide a method for cultivating and preparing highly efficient active biochemical bacteria to solve the needs of beneficial microorganisms in production, biochemically degrade cellulose, hemicellulose, and lignin, and increase the crude protein and amino acid content of straw The technical solution to this problem is: add the collected rumen content or gastric juice of ruminant livestock to water, inoculate it in a culture medium composed of salt, licorice powder, ammonium bicarbonate, etc. Carry out anaerobic fermentation under and pH value, add water and culture medium again after multiplying, carry out secondary biochemical under the same condition as primary biochemical to make active biochemical bacteria (liquid). The method of the present invention is simple, and cultivation effect is good , its biochemical bacteria are widely used, have great value, have broad development and application prospects and huge economic benefits

Method used

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Embodiment Construction

[0004] The specific implementation manners of the present invention will be described in detail below in combination with actual conditions.

[0005] The present invention firstly extracts rumen juice, which refers to the rumen content or gastric juice of ruminant livestock, which is obtained directly from the stomach of slaughtered healthy ruminant livestock or sucked out from the rumen of healthy ruminant livestock with a catheter, and then added with water to expand the bacteria , and then add the culture medium to carry out the graded original bacteria expansion culture (the beneficial active biochemical bacteria contained in the rumen fluid are called original bacteria), first the first-level biochemical expansion culture, and then carry out the second-level biochemical and strain subculture, the said The first-level biochemical original bacteria expansion culture is to put water at a temperature of 45°C in the seed tank and 6 times the volume of rumen fluid to make a wate...

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Abstract

The present invention is process of cultivating and preparing active biochemical bacteria for degrading cellulose, hemicellulose and lignin biochemically to raise the content of stalk coarse protein and amino acids. The process of cultivating and preparing active biochemical bacteria includes the following steps: adding water to rumen content or rumen juice collected from ruminant; inoculating to the culture medium comprising table salt, licorice powder, ammonium bicarbonate, etc; the first anaerobic fermentation to proliferate; adding water and culture medium and the second fermentation to obtain the active biochemical bacteria liquid product. The active biochemical bacteria liquid product has wide application foreground.

Description

1. Technical field [0001] The invention relates to a method for cultivating and preparing high-efficiency active biochemical bacteria with multiple functions. 2. Background technology [0002] Agricultural microorganisms have broad application prospects in the fields of crop planting, livestock and poultry breeding, aquaculture, agricultural product processing, agricultural environmental protection, and agricultural production technology. At present, the application of microbial technology in agricultural production in developed countries is very extensive. From the application of single strains in the past to the development of highly active bacteria, it is widely used in the production of bio-organic fertilizers, bio-pesticides and bio-feeds, for the preservation of agricultural products or for environmental protection. (Sewage waste treatment), such as Japan's three major brands of enzyme bacteria, EM, and Wantian fermentation. At present, there are more than 3,000 strai...

Claims

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Application Information

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IPC IPC(8): C12N1/00
Inventor 曹明军陈万朝曹爽
Owner 南阳兴农生物技术开发有限公司
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