A cell mediated immunity vaccine and preparation method thereof

A technology of cellular immunity and vaccines, applied in pharmaceutical formulas, medical preparations containing active ingredients, antibody medical ingredients, etc., can solve problems such as uncontrollable diseases and lagging vaccines, and achieve significant curative effect, low cost, and fast results

Inactive Publication Date: 2007-04-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main object of vaccine research is the pathogenic source (or pathogen, etc.) that causes the disease. However, due to the rapid mutation rate of the pathogen, the speed of vaccine development is far behind, which often results in the uncontrollable early stage of the epidemic. pay a heavy price

Method used

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  • A cell mediated immunity vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the preparation of pullorumosis vaccine and its detection

[0028] 1. Prepare the vaccine

[0029] 1. Acquisition and purification of mononuclear cells

[0030] 1) Isolation of mononuclear cells

[0031] Isolate mononuclear cells from chickens. The specific method is: sterilize the blood collection site under the chicken wings, collect blood from the vein, add heparin sodium solution with a volume percentage concentration of 5% to anticoagulate, and then use lymphocyte separation solution from the anticoagulant blood (purchased from Cedarlane Company) to obtain mononuclear cells by density gradient centrifugation.

[0032] 2) Purification of mononuclear cells

[0033] Mononuclear cells were cultured in complete RPMI1640 medium at 37°C, 5% CO 2 Cultivate in a constant humidity incubator for 8-24 hours. After the cells adhere to the wall, remove the suspended cells, and carry out acridine orange (AO) staining, Giemsa staining and CD14 indirect immunofluo...

Embodiment 2

[0044] Embodiment 2, preparation and detection of porcine erysipelas vaccine

[0045] 1. Prepare the vaccine

[0046] 1. Acquisition and purification of mononuclear cells

[0047] 1) Isolation of mononuclear cells

[0048] The specific method for isolating mononuclear cells from pigs is as follows: sterilize the blood collection site of the anterior vena cava of pigs, collect blood from the vein, add sodium citrate solution with a volume percentage concentration of 5% to anticoagulate blood, and then use lymphocytes from the anticoagulant blood Mononuclear cells were obtained from the separation solution by density gradient centrifugation.

[0049] 2) Purification of mononuclear cells

[0050] The mononuclear cells were cultured with RPMI1640 complete culture medium in a 37°C incubator for 24-48 hours. After the cells adhered to the wall, the suspended cells were removed, and the adherent cells were stained with acridine orange (AO), Giemsa staining and CD14 The results of...

Embodiment 3

[0061] Embodiment 3, preparation and detection of bovine tuberculosis vaccine

[0062] 1. Prepare the vaccine

[0063] 1. Acquisition and purification of mononuclear cells

[0064] 1) Isolation of mononuclear cells

[0065] Mononuclear cells are isolated from cattle. The specific method is: sterilize the human venous blood collection site, collect blood from the vein, add sodium citrate solution with a volume concentration of 5% to anticoagulate the blood, and then use the lymphocyte separation fluid to pass through the density of the anticoagulated blood. Mononuclear cells were isolated by gradient centrifugation.

[0066] 2) Purification of mononuclear cells

[0067] Culture mononuclear cells with TCM199 complete culture medium in a 37°C incubator for 24-72 hours. After the cells adhere to the wall, remove the suspended cells, and perform acridine orange (AO) staining, Giemsa staining and CD14 staining on the adherent cells. The results of indirect immunofluorescence sta...

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Abstract

The invention relates to a cell immunity vaccine and relative preparation. Wherein, said vaccine is the immunity cell cultivated from mono/macrophage or macrophage with single or several causal agents or other components. The inventive vaccine has following advantages that: (1), it has high immunity; (2), it has high safety; (3), it has wide spectrum; (4), it has significant effect. The invention has simple process and low cost, with wide application.

Description

technical field [0001] The invention relates to a vaccine and a preparation method thereof, in particular to a vaccine which has preventive and protective effects on various infectious diseases at the cell level and a preparation method thereof. Background technique [0002] Various infectious diseases seriously threaten the health of animals and humans, and in the 21st century, new diseases continue to challenge humans. From SARS in 2003 to the current highly pathogenic avian influenza, they have seriously threatened human health and even life. In addition, tumors and autoimmune diseases are chronic diseases that pose a huge threat to human health. Therefore, there is an urgent need for a vaccine with preventive and / or therapeutic effects on infectious diseases and / or tumors. [0003] In addition, terrorist attacks using highly lethal pathogens will cause great panic and threats to human society. For example, the use of anthrax has sounded the alarm for the United States ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K39/00A61K35/12A61P31/00A61K35/14A61K35/57
Inventor 连正兴邹炜李海
Owner CHINA AGRI UNIV
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