Biologic bactericide and preparation method

A technology of biological fungicides and bacterial strains, applied in botany equipment and methods, fungicides, biocides, etc., can solve the problems of poor control stability, different effects, slow effects, etc.

Inactive Publication Date: 2007-05-09
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In practice, it is found that biopesticide fungicides have disadvantages such as poor control stability and slow action effect. A large number of practices have proved that the reason for the above slow action effect is that the survival rate of living cells and their metabolic strength after the application of microbial fungicides are different. It is closely related to the environment of the application site, and the difference in the environment will bring about the difference in the effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation method of the biological bactericide, the technological process includes: slant bacteria activation culture of new strains - shaker culture - seed tank culture - fermenter tank culture - fermented liquid sedimentation to remove residues - adding stabilizers - finished product packaging, The specific requirement is: when carrying out the slant strain culture process, the newly screened Streptomyces roseoflaver (Streptomyces. Its medium formula mainly includes starch, corn steep liquor and appropriate amount of potassium dihydrogen phosphate (KH 2 PO 4 ), sodium chloride (NaCl), magnesium sulfate (MgSO 4 ), ferrous sulfate (FeSO 4 ), calcium carbonate (CaCO 3 ), and the rest is clean water. In the formula, the starch and corn steep liquor dry matter account for 1.5% of the starch and 2% of the corn steep liquor dry matter in the weight percentage of the culture medium.

[0057] When carrying out the fermentation tank cultivation process, the stirring ...

Embodiment 2

[0062] The preparation method of the biological bactericide, the technological process includes: slant bacteria activation culture of new strains - shaker culture - seed tank culture - fermenter tank culture - fermented liquid sedimentation to remove residues - adding stabilizers - finished product packaging, The specific requirements are: a. During the slant culture process, the newly screened mutagenic strain 0116 of Streptomyces roseoflaver is used for activation and culture. Its medium formula mainly includes starch, corn steep liquor and appropriate amount of potassium dihydrogen phosphate (KH 2 PO 4 ), sodium chloride (NaCl), magnesium sulfate (MgSO 4 ), ferrous sulfate (FeSO 4 ), calcium carbonate (CaCO 3 ), and the rest is clean water. In the formula, the starch and the dry matter of corn steep liquor account for 2.5% of the starch and 3% of the dry matter of the corn steep liquor in the culture medium.

[0063] When carrying out the fermentation tank cultivation ...

Embodiment 3

[0068] The preparation method of the biological bactericide, the technological process includes: slant bacteria activation culture of new strains - shaker culture - seed tank culture - fermenter tank culture - fermented liquid sedimentation to remove residues - adding stabilizers - finished product packaging, The specific requirement is: when carrying out the slant strain culture process, the newly screened Streptomyces roseoflaver (Streptomyces. Its medium formula mainly includes starch, corn steep liquor and appropriate amount of potassium dihydrogen phosphate (KH 2 PO 4 ), sodium chloride (NaCl), magnesium sulfate (MgSO 4 ), ferrous sulfate (FeSO 4 ), calcium carbonate (CaCO 3 ), and the rest is clean water. In the formula, the starch and the dry matter of corn steep liquor account for 2.5% of the starch and 3% of the dry matter of the corn steep liquor in the culture medium.

[0069] When carrying out the fermentation tank cultivation process, the stirring speed in th...

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PUM

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Abstract

A biologic bactericide is prepared from the fermented liquid of the variant 0116 of Streptomyces roseoflaver, phenylethylphenol polyoxyvinylether, sodium acetate and acetic acid through slant culture, shaker culture, seeding tank culture, fermentor culture, depositing the fermented liquid, removing residual dregs, adding stabilizer and packing.

Description

Technical field: [0001] The invention relates to a biological bactericide for preventing and treating fruit thinning diseases, and also relates to a preparation method of the biological bactericide. Background technique [0002] The vast majority of traditional fungicides use chemical pesticides, which use the action of chemical substances in antibiotics to inhibit and kill germs. The production and use of such products will inevitably bring about pollution and pesticide residues and other phytotoxicity problems. For example, long-term frequent use of chemical pesticides can lead to resistance of bacteria and insect pests, and gradually reduce the efficacy of chemical pesticides themselves. In order to achieve the purpose of sterilization, it is necessary to increase the dosage. This will not only increase agricultural costs, but also increase the amount of pesticide residues, which is very unfavorable to human health and seriously pollutes the environment; due to the broad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00
Inventor 刘大群赤国彤孟庆芳李亚宁张立荣
Owner HEBEI AGRICULTURAL UNIV.
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