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Polyamino acid chemical-modification bacterium and its preparation method and use

A technology of polyamino acid chemistry and drying bacteria, applied in biochemical equipment and methods, chemical instruments and methods, fixed on/in organic carriers, etc., can solve the problem of loose surface structure, poor mechanical strength, and weak adsorption capacity and other problems, to achieve the effect of good regeneration performance, high adsorption efficiency, and simple production method

Inactive Publication Date: 2007-05-30
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the actual process, microbial adsorbents are often limited in their specific applications due to their loose surface structure, poor mechanical strength, and weak adsorption capacity.

Method used

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  • Polyamino acid chemical-modification bacterium and its preparation method and use
  • Polyamino acid chemical-modification bacterium and its preparation method and use
  • Polyamino acid chemical-modification bacterium and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Synthesis of polyamino acid modified yeast

[0043] 1. First add 15g of dry yeast to 75mL of 1.0% (w / w) glutaraldehyde aqueous solution, shake and react for 14 hours, centrifuge, wash with water, and dry to obtain glutaraldehyde-crosslinked bacteria;

[0044] 2. Dissolve 0.078mol of pyromellitic dianhydride and 0.026mol of arginine in 100mL of N,N-dimethylformamide, stir at room temperature for 1-2 hours, then add 6.0g of glutaraldehyde cross-linking bacteria, After continuing to react at 40-50°C for 2 hours, centrifuge and wash the product 4 times with N,N-dimethylformamide;

[0045] 3. Add the yeast obtained above to 50mL 0.1mol L -1 Alkaline in NaOH solution for 0.5 hour, centrifuge, wash until neutral, and freeze-dry to obtain polyamino acid-modified yeast.

Embodiment 2

[0046] Example 2: Polyamino acid modified yeast synthesis

[0047] 1. First add 15g of dry yeast to 100mL of 1.0% (w / w) glutaraldehyde aqueous solution, shake and react for 15 hours, centrifuge, wash with water, and dry to obtain glutaraldehyde-crosslinked bacteria;

[0048] 2. Dissolve 0.208mol of pyromellitic dianhydride and 0.026mol of thiourea in 100mL of N,N-dimethylformamide, stir at room temperature for 2 hours, add 6.0g of glutaraldehyde cross-linking bacteria, 40-50 After continuing to react at ℃ for 3 hours, centrifuge and wash the product 4 times with N,N-dimethylformamide;

[0049] 3. Add the yeast obtained above to 50mL 0.1mol L -1 Alkaline in NaOH solution for 1 hour, centrifuge, wash until neutral, and freeze-dry to obtain polyamino acid-modified yeast.

Embodiment 3

[0050] Embodiment 3: Synthesis of polyamino acid modified yeast

[0051] 1. First add 15.0g of dry yeast into 150mL of 1.0% (w / w) glutaraldehyde aqueous solution, shake and react for 16 hours, centrifuge, wash with water, and dry;

[0052] 2. Dissolve 0.14mol of pyromellitic dianhydride and 0.026mol of lysine in 100mL of N,N-dimethylformamide, stir at room temperature for 2 hours, add 6.0g of glutaraldehyde cross-linking bacteria, 40- After continuing to react at 50°C for 4 hours, centrifuge and wash the product 4 times with N,N-dimethylformamide;

[0053] 3. Add the yeast obtained above to 50mL 0.1mol L -1 Alkaline in NaOH solution for 0.75 hours, centrifuge, wash until neutral, and freeze-dry to obtain polyamino acid-modified yeast.

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Abstract

The invention discloses a making method of polyamine acid chemical decorative bacteria, which comporises the following steps: (a) adding inactivated xerosin into solution with 1-10% crosslinking agent; vibrating 10-20h; washing; drying; obtaining crosslinking bacteria; (b) dissolving dianhydride and diamine material in the N,N-dialkyl amide; stirring under normal temperature for 1-2h; (c) adding synthesized bacteria into alkali solution to alkalize 0.5-1h; centrifuging; washing to neutral; freezing; drying to obtain the product. The invention improves the adsorbing quantity of Pb2+ and Cd2+ by 10 times corresponding to non-decorated yeast.

Description

technical field [0001] The invention relates to the interdisciplinary fields of microbial science, solid-phase organic synthesis and environmental science, and more specifically relates to polyamine chemically modified bacteria, its preparation method and application. Background technique [0002] Since the 1970s, a large number of studies on biosorption have been carried out. The adsorption mechanism of microorganisms to metal ions is very complicated. The functional groups on the surface, such as sulfhydryl, carboxyl, hydroxyl, amino, etc., can combine with metal ions and enrich the solution through ion exchange, complexation, synergy, chelation, physical adsorption, etc. Metal. Microorganisms have a wide range of sources and low prices. Compared with other non-biological adsorbents, they have the advantages of fast adsorption, wide pH and temperature ranges, and easy regeneration. In the actual application process, metal resources can be recovered from wastewater, and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N11/04C02F1/62C02F3/00C02F1/28
Inventor 李步海孙小梅余军霞佟蜜雷超
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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