The invention provides a formula feed for laying hens during an egg-laying peak period and a preparation method of the formula feed. The formula feed comprises the following components in percentage by weight: 40-50% of corns, 8-12% of soybean meal, 1-3% of saccharomyces cerevisia fermented liquor dregs, 20-30% of corn distiller dried grains, rice bran, stone dust, zeolite powder, calcium hydrogen phosphate, common salt, soybean lecithin oil, lysine, methionine, choline chloride, soybean Isoflavones, sodium bicarbonate, a compound premix and the like. The application provides the formula feed for the laying hens, which is comprehensive in nutrients, easy to digest, easy to absorb and stress-resistant, and can obviously reduce various stresses, improve the health of intestines, improve the immunity of organisms of the laying hens, maintain the stability of the egg laying rate and reduce the cost of the feed and the cultivation cost. Meanwhile, the apparent digestion rate of crude proteins, calcium, phosphorus and other nutritional substances is significantly improved, the emission of nitrogen and phosphorus in manure is reduced, and the environmental pollution caused by excretion of manure can be obviously reduced.